Strain Fitness in Variovorax sp. SCN45 around GFF4750

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4748 and GFF4749 are separated by 39 nucleotidesGFF4749 and GFF4750 are separated by 241 nucleotidesGFF4750 and GFF4751 are separated by 15 nucleotidesGFF4751 and GFF4752 are separated by 13 nucleotides GFF4748 - Triosephosphate isomerase (EC 5.3.1.1), at 115,892 to 116,656 GFF4748 GFF4749 - Protein translocase membrane subunit SecG, at 116,696 to 117,106 GFF4749 GFF4750 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 117,348 to 117,737 GFF4750 GFF4751 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 117,753 to 118,232 GFF4751 GFF4752 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3), at 118,246 to 118,857 GFF4752 Position (kb) 117 118Strain fitness (log2 ratio) -3 -2 -1 0 1at 116.396 kb on + strand, within GFF4748at 116.396 kb on + strand, within GFF4748at 116.681 kb on + strandat 116.918 kb on + strand, within GFF4749at 117.104 kb on + strandat 117.104 kb on + strandat 117.104 kb on + strandat 117.104 kb on + strandat 117.104 kb on + strandat 117.162 kb on + strandat 117.162 kb on + strandat 117.747 kb on + strandat 117.747 kb on + strandat 117.747 kb on + strandat 117.748 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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116,396 + GFF4748 0.66 -2.0
116,396 + GFF4748 0.66 -2.9
116,681 + -0.4
116,918 + GFF4749 0.54 +0.5
117,104 + +1.6
117,104 + +0.1
117,104 + -0.5
117,104 + -0.5
117,104 + +0.7
117,162 + -0.3
117,162 + -1.0
117,747 + +0.2
117,747 + -0.0
117,747 + -0.2
117,748 - +0.5

Or see this region's nucleotide sequence