Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4748 and GFF4749 are separated by 39 nucleotides GFF4749 and GFF4750 are separated by 241 nucleotides GFF4750 and GFF4751 are separated by 15 nucleotides GFF4751 and GFF4752 are separated by 13 nucleotides
GFF4748 - Triosephosphate isomerase (EC 5.3.1.1), at 115,892 to 116,656
GFF4748
GFF4749 - Protein translocase membrane subunit SecG, at 116,696 to 117,106
GFF4749
GFF4750 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 117,348 to 117,737
GFF4750
GFF4751 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 117,753 to 118,232
GFF4751
GFF4752 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3), at 118,246 to 118,857
GFF4752
Position (kb)
117
118 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 116.396 kb on + strand, within GFF4748 at 116.396 kb on + strand, within GFF4748 at 116.681 kb on + strand at 116.918 kb on + strand, within GFF4749 at 117.104 kb on + strand at 117.104 kb on + strand at 117.104 kb on + strand at 117.104 kb on + strand at 117.104 kb on + strand at 117.162 kb on + strand at 117.162 kb on + strand at 117.747 kb on + strand at 117.747 kb on + strand at 117.747 kb on + strand at 117.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 116,396 + GFF4748 0.66 -2.0 116,396 + GFF4748 0.66 -2.9 116,681 + -0.4 116,918 + GFF4749 0.54 +0.5 117,104 + +1.6 117,104 + +0.1 117,104 + -0.5 117,104 + -0.5 117,104 + +0.7 117,162 + -0.3 117,162 + -1.0 117,747 + +0.2 117,747 + -0.0 117,747 + -0.2 117,748 - +0.5
Or see this region's nucleotide sequence