Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4737 and GFF4738 are separated by 330 nucleotides GFF4738 and GFF4739 are separated by 79 nucleotides
GFF4737 - Nitrate ABC transporter, substrate-binding protein, at 102,984 to 104,228
GFF4737
GFF4738 - Response regulator NasT, at 104,559 to 105,875
GFF4738
GFF4739 - N(6)-L-threonylcarbamoyladenine synthase (EC 2.3.1.234), at 105,955 to 107,013
GFF4739
Position (kb) 104
105
106 Strain fitness (log2 ratio) -2
-1
0
1 at 103.655 kb on - strand, within GFF4737 at 103.702 kb on + strand, within GFF4737 at 103.702 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.750 kb on + strand, within GFF4737 at 103.751 kb on - strand, within GFF4737 at 103.751 kb on - strand, within GFF4737 at 103.913 kb on - strand, within GFF4737 at 103.913 kb on - strand, within GFF4737 at 103.924 kb on + strand, within GFF4737 at 103.924 kb on + strand, within GFF4737 at 103.925 kb on - strand, within GFF4737 at 104.017 kb on + strand, within GFF4737 at 104.018 kb on - strand, within GFF4737 at 104.018 kb on - strand, within GFF4737 at 104.399 kb on + strand at 104.399 kb on + strand at 104.399 kb on + strand at 104.399 kb on + strand at 104.399 kb on + strand at 104.400 kb on - strand at 104.400 kb on - strand at 104.400 kb on - strand at 104.593 kb on + strand at 104.593 kb on + strand at 104.593 kb on + strand at 104.593 kb on + strand at 105.744 kb on + strand at 105.744 kb on + strand at 105.744 kb on + strand at 105.745 kb on - strand at 105.942 kb on + strand at 105.943 kb on - strand at 105.943 kb on - strand at 105.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 103,655 - GFF4737 0.54 +0.4 103,702 + GFF4737 0.58 -0.1 103,702 + GFF4737 0.58 +1.5 103,750 + GFF4737 0.62 +0.4 103,750 + GFF4737 0.62 -1.2 103,750 + GFF4737 0.62 -2.3 103,750 + GFF4737 0.62 +0.3 103,751 - GFF4737 0.62 -0.9 103,751 - GFF4737 0.62 +0.7 103,913 - GFF4737 0.75 -0.9 103,913 - GFF4737 0.75 +1.3 103,924 + GFF4737 0.76 +0.3 103,924 + GFF4737 0.76 -0.0 103,925 - GFF4737 0.76 +0.3 104,017 + GFF4737 0.83 +0.7 104,018 - GFF4737 0.83 -1.0 104,018 - GFF4737 0.83 +1.3 104,399 + +0.1 104,399 + -0.5 104,399 + +0.7 104,399 + -0.2 104,399 + +0.7 104,400 - +0.4 104,400 - -0.1 104,400 - +0.1 104,593 + +1.0 104,593 + -0.4 104,593 + -0.4 104,593 + +0.6 105,744 + -1.4 105,744 + -0.5 105,744 + +0.3 105,745 - -0.1 105,942 + +0.6 105,943 - +0.5 105,943 - -0.4 105,943 - -0.8
Or see this region's nucleotide sequence