Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4731 and GFF4732 are separated by 48 nucleotides GFF4732 and GFF4733 are separated by 9 nucleotides GFF4733 and GFF4734 overlap by 4 nucleotides
GFF4731 - Assimilatory nitrate reductase large subunit (EC 1.7.99.4), at 94,717 to 97,509
GFF4731
GFF4732 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 97,558 to 97,953
GFF4732
GFF4733 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 97,963 to 98,754
GFF4733
GFF4734 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 98,751 to 101,219
GFF4734
Position (kb)
97
98 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 96.620 kb on + strand, within GFF4731 at 96.621 kb on - strand, within GFF4731 at 96.621 kb on - strand, within GFF4731 at 96.621 kb on - strand, within GFF4731 at 96.718 kb on - strand, within GFF4731 at 96.718 kb on - strand, within GFF4731 at 96.718 kb on - strand, within GFF4731 at 96.773 kb on + strand, within GFF4731 at 96.774 kb on - strand, within GFF4731 at 96.774 kb on - strand, within GFF4731 at 96.774 kb on - strand, within GFF4731 at 96.774 kb on - strand, within GFF4731 at 97.029 kb on - strand, within GFF4731 at 97.067 kb on + strand, within GFF4731 at 97.068 kb on - strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.190 kb on + strand, within GFF4731 at 97.191 kb on - strand, within GFF4731 at 97.481 kb on + strand at 97.560 kb on - strand at 97.964 kb on + strand at 97.964 kb on + strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.982 kb on + strand at 97.983 kb on - strand at 98.258 kb on + strand, within GFF4733 at 98.259 kb on - strand, within GFF4733 at 98.345 kb on + strand, within GFF4733 at 98.480 kb on + strand, within GFF4733 at 98.481 kb on - strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.921 kb on - strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.945 kb on - strand at 98.945 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 96,620 + GFF4731 0.68 -0.5 96,621 - GFF4731 0.68 -0.2 96,621 - GFF4731 0.68 -0.0 96,621 - GFF4731 0.68 -0.7 96,718 - GFF4731 0.72 -0.8 96,718 - GFF4731 0.72 +0.2 96,718 - GFF4731 0.72 -0.3 96,773 + GFF4731 0.74 +0.6 96,774 - GFF4731 0.74 +0.9 96,774 - GFF4731 0.74 -0.0 96,774 - GFF4731 0.74 -1.4 96,774 - GFF4731 0.74 -1.4 97,029 - GFF4731 0.83 -0.7 97,067 + GFF4731 0.84 +1.3 97,068 - GFF4731 0.84 -0.3 97,157 + GFF4731 0.87 -0.1 97,157 + GFF4731 0.87 -2.2 97,157 + GFF4731 0.87 -0.1 97,158 - GFF4731 0.87 +0.2 97,158 - GFF4731 0.87 -1.2 97,158 - GFF4731 0.87 -0.3 97,160 + GFF4731 0.87 -0.4 97,160 + GFF4731 0.87 +0.1 97,160 + GFF4731 0.87 -0.0 97,160 + GFF4731 0.87 -0.6 97,160 + GFF4731 0.87 -1.3 97,160 + GFF4731 0.87 +0.4 97,161 - GFF4731 0.88 -0.4 97,161 - GFF4731 0.88 -0.5 97,161 - GFF4731 0.88 -0.7 97,161 - GFF4731 0.88 +0.8 97,161 - GFF4731 0.88 +0.3 97,161 - GFF4731 0.88 -0.2 97,190 + GFF4731 0.89 -0.1 97,191 - GFF4731 0.89 +1.1 97,481 + -0.8 97,560 - -0.5 97,964 + -0.3 97,964 + +0.2 97,965 - -0.7 97,965 - -0.6 97,965 - +0.7 97,965 - +1.7 97,982 + -0.1 97,983 - +0.7 98,258 + GFF4733 0.37 +0.1 98,259 - GFF4733 0.37 -0.4 98,345 + GFF4733 0.48 -0.8 98,480 + GFF4733 0.65 -0.3 98,481 - GFF4733 0.65 +0.6 98,609 + GFF4733 0.82 +1.3 98,609 + GFF4733 0.82 -0.1 98,610 - GFF4733 0.82 -1.5 98,610 - GFF4733 0.82 +0.7 98,921 - -1.2 98,944 + -0.9 98,944 + +1.4 98,944 + -1.0 98,945 - -0.3 98,945 - +2.1
Or see this region's nucleotide sequence