Strain Fitness in Variovorax sp. SCN45 around GFF470

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF468 and GFF469 overlap by 31 nucleotidesGFF469 and GFF470 overlap by 4 nucleotidesGFF470 and GFF471 are separated by 101 nucleotides GFF468 - Transcriptional regulator, LysR family, at 506,422 to 507,360 GFF468 GFF469 - hypothetical protein, at 507,330 to 507,869 GFF469 GFF470 - Transcriptional regulator, LysR family, at 507,866 to 508,840 GFF470 GFF471 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 508,942 to 510,636 GFF471 Position (kb) 507 508 509Strain fitness (log2 ratio) -1 0 1 2at 506.902 kb on + strand, within GFF468at 506.902 kb on + strand, within GFF468at 506.902 kb on + strand, within GFF468at 506.903 kb on - strand, within GFF468at 506.965 kb on + strand, within GFF468at 506.965 kb on + strand, within GFF468at 506.965 kb on + strand, within GFF468at 506.965 kb on + strand, within GFF468at 506.965 kb on + strand, within GFF468at 506.965 kb on + strand, within GFF468at 506.965 kb on + strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 506.966 kb on - strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.073 kb on + strand, within GFF468at 507.074 kb on - strand, within GFF468at 507.074 kb on - strand, within GFF468at 507.074 kb on - strand, within GFF468at 507.074 kb on - strand, within GFF468at 507.074 kb on - strand, within GFF468at 507.121 kb on + strand, within GFF468at 507.121 kb on + strand, within GFF468at 507.122 kb on - strand, within GFF468at 507.122 kb on - strand, within GFF468at 507.122 kb on - strand, within GFF468at 507.122 kb on - strand, within GFF468at 507.208 kb on + strand, within GFF468at 507.209 kb on - strand, within GFF468at 507.209 kb on - strand, within GFF468at 507.403 kb on + strand, within GFF469at 507.403 kb on + strand, within GFF469at 507.404 kb on - strand, within GFF469at 507.484 kb on + strand, within GFF469at 507.484 kb on + strand, within GFF469at 507.484 kb on + strand, within GFF469at 507.485 kb on - strand, within GFF469at 507.532 kb on + strand, within GFF469at 507.640 kb on + strand, within GFF469at 507.640 kb on + strand, within GFF469at 507.640 kb on + strand, within GFF469at 507.640 kb on + strand, within GFF469at 507.640 kb on + strand, within GFF469at 507.641 kb on - strand, within GFF469at 507.641 kb on - strand, within GFF469at 507.748 kb on + strand, within GFF469at 507.748 kb on + strand, within GFF469at 507.749 kb on - strand, within GFF469at 507.766 kb on + strand, within GFF469at 507.870 kb on - strandat 508.053 kb on + strand, within GFF470at 508.053 kb on + strand, within GFF470at 508.053 kb on + strand, within GFF470at 508.054 kb on - strand, within GFF470at 508.054 kb on - strand, within GFF470at 508.191 kb on + strand, within GFF470at 508.191 kb on + strand, within GFF470at 508.192 kb on - strand, within GFF470at 508.192 kb on - strand, within GFF470at 508.192 kb on - strand, within GFF470at 508.192 kb on - strand, within GFF470at 508.851 kb on + strandat 508.901 kb on + strandat 508.901 kb on + strandat 508.902 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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506,902 + GFF468 0.51 -0.7
506,902 + GFF468 0.51 +0.4
506,902 + GFF468 0.51 +0.2
506,903 - GFF468 0.51 +0.0
506,965 + GFF468 0.58 -0.3
506,965 + GFF468 0.58 +0.2
506,965 + GFF468 0.58 +1.4
506,965 + GFF468 0.58 -0.2
506,965 + GFF468 0.58 +0.2
506,965 + GFF468 0.58 +1.0
506,965 + GFF468 0.58 +0.6
506,966 - GFF468 0.58 -0.5
506,966 - GFF468 0.58 -0.5
506,966 - GFF468 0.58 -0.4
506,966 - GFF468 0.58 -0.1
506,966 - GFF468 0.58 -0.8
506,966 - GFF468 0.58 +0.9
506,966 - GFF468 0.58 +0.1
506,966 - GFF468 0.58 -0.0
506,966 - GFF468 0.58 +0.2
506,966 - GFF468 0.58 -0.1
506,966 - GFF468 0.58 -0.4
507,073 + GFF468 0.69 -0.4
507,073 + GFF468 0.69 -0.0
507,073 + GFF468 0.69 +0.3
507,073 + GFF468 0.69 -0.0
507,073 + GFF468 0.69 +1.0
507,073 + GFF468 0.69 -0.3
507,073 + GFF468 0.69 -0.9
507,073 + GFF468 0.69 +0.1
507,073 + GFF468 0.69 -0.4
507,073 + GFF468 0.69 +0.6
507,073 + GFF468 0.69 -0.8
507,073 + GFF468 0.69 -0.2
507,073 + GFF468 0.69 +0.2
507,073 + GFF468 0.69 +0.2
507,073 + GFF468 0.69 +0.1
507,073 + GFF468 0.69 -0.9
507,074 - GFF468 0.69 +1.1
507,074 - GFF468 0.69 -0.4
507,074 - GFF468 0.69 +0.9
507,074 - GFF468 0.69 +0.1
507,074 - GFF468 0.69 -0.5
507,121 + GFF468 0.74 +0.3
507,121 + GFF468 0.74 +0.6
507,122 - GFF468 0.75 -0.1
507,122 - GFF468 0.75 -1.0
507,122 - GFF468 0.75 -1.0
507,122 - GFF468 0.75 +1.0
507,208 + GFF468 0.84 +2.2
507,209 - GFF468 0.84 +0.1
507,209 - GFF468 0.84 -0.4
507,403 + GFF469 0.14 -1.4
507,403 + GFF469 0.14 -0.5
507,404 - GFF469 0.14 -0.6
507,484 + GFF469 0.29 -0.0
507,484 + GFF469 0.29 -0.2
507,484 + GFF469 0.29 -1.0
507,485 - GFF469 0.29 -0.3
507,532 + GFF469 0.37 +0.5
507,640 + GFF469 0.57 +0.3
507,640 + GFF469 0.57 +0.0
507,640 + GFF469 0.57 -1.1
507,640 + GFF469 0.57 +1.2
507,640 + GFF469 0.57 -0.1
507,641 - GFF469 0.58 +0.5
507,641 - GFF469 0.58 -0.3
507,748 + GFF469 0.77 -1.2
507,748 + GFF469 0.77 -0.1
507,749 - GFF469 0.78 -1.1
507,766 + GFF469 0.81 +0.0
507,870 - -0.1
508,053 + GFF470 0.19 +0.2
508,053 + GFF470 0.19 -0.8
508,053 + GFF470 0.19 -1.7
508,054 - GFF470 0.19 -0.5
508,054 - GFF470 0.19 +0.2
508,191 + GFF470 0.33 -1.6
508,191 + GFF470 0.33 -0.9
508,192 - GFF470 0.33 +0.3
508,192 - GFF470 0.33 +0.0
508,192 - GFF470 0.33 -0.8
508,192 - GFF470 0.33 +0.2
508,851 + -0.1
508,901 + +1.0
508,901 + +0.0
508,902 - +2.2

Or see this region's nucleotide sequence