Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF463 and GFF464 are separated by 28 nucleotides GFF464 and GFF465 are separated by 112 nucleotides GFF465 and GFF466 are separated by 63 nucleotides GFF466 and GFF467 are separated by 32 nucleotides
GFF463 - Malonyl-CoA decarboxylase (EC 4.1.1.9), at 502,039 to 503,505
GFF463
GFF464 - Transcriptional regulator, GntR family, at 503,534 to 504,202
GFF464
GFF465 - Prolipoprotein diacylglyceryl transferase, at 504,315 to 505,139
GFF465
GFF466 - hypothetical protein, at 505,203 to 505,472
GFF466
GFF467 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 505,505 to 506,278
GFF467
Position (kb) 504
505
506 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 503.732 kb on + strand, within GFF464 at 503.732 kb on + strand, within GFF464 at 503.733 kb on - strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.820 kb on + strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.821 kb on - strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.823 kb on + strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.824 kb on - strand, within GFF464 at 503.923 kb on - strand, within GFF464 at 503.923 kb on - strand, within GFF464 at 504.230 kb on + strand at 504.237 kb on - strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.296 kb on + strand at 504.297 kb on - strand at 504.297 kb on - strand at 504.297 kb on - strand at 504.297 kb on - strand at 505.258 kb on - strand, within GFF466 at 505.258 kb on - strand, within GFF466 at 505.518 kb on + strand at 505.518 kb on + strand at 505.518 kb on + strand at 505.519 kb on - strand at 505.519 kb on - strand at 505.519 kb on - strand at 505.656 kb on + strand, within GFF467 at 505.656 kb on + strand, within GFF467 at 505.657 kb on - strand, within GFF467 at 505.686 kb on + strand, within GFF467 at 505.687 kb on - strand, within GFF467 at 505.687 kb on - strand, within GFF467 at 505.803 kb on + strand, within GFF467 at 505.803 kb on + strand, within GFF467 at 505.803 kb on + strand, within GFF467 at 505.803 kb on + strand, within GFF467 at 505.812 kb on + strand, within GFF467 at 505.812 kb on + strand, within GFF467 at 505.812 kb on + strand, within GFF467 at 505.812 kb on + strand, within GFF467 at 505.813 kb on - strand, within GFF467 at 505.813 kb on - strand, within GFF467 at 505.813 kb on - strand, within GFF467 at 505.825 kb on - strand, within GFF467 at 505.942 kb on - strand, within GFF467 at 505.942 kb on - strand, within GFF467
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 503,732 + GFF464 0.30 -0.8 503,732 + GFF464 0.30 -0.2 503,733 - GFF464 0.30 -2.3 503,820 + GFF464 0.43 -0.6 503,820 + GFF464 0.43 +0.5 503,820 + GFF464 0.43 -0.2 503,820 + GFF464 0.43 -1.0 503,820 + GFF464 0.43 -0.3 503,820 + GFF464 0.43 +1.8 503,821 - GFF464 0.43 -0.2 503,821 - GFF464 0.43 -0.6 503,821 - GFF464 0.43 -0.6 503,821 - GFF464 0.43 -1.7 503,821 - GFF464 0.43 +0.4 503,821 - GFF464 0.43 -0.6 503,821 - GFF464 0.43 -0.3 503,823 + GFF464 0.43 -0.1 503,823 + GFF464 0.43 -0.2 503,823 + GFF464 0.43 +0.3 503,823 + GFF464 0.43 +0.1 503,823 + GFF464 0.43 -0.7 503,823 + GFF464 0.43 +0.4 503,824 - GFF464 0.43 -1.2 503,824 - GFF464 0.43 +0.5 503,824 - GFF464 0.43 -0.4 503,824 - GFF464 0.43 -1.6 503,824 - GFF464 0.43 -0.9 503,923 - GFF464 0.58 -0.6 503,923 - GFF464 0.58 +0.1 504,230 + +0.7 504,237 - +1.8 504,296 + -0.8 504,296 + -0.2 504,296 + -0.1 504,296 + +0.4 504,296 + +0.0 504,296 + -0.1 504,297 - +0.6 504,297 - -2.1 504,297 - +2.0 504,297 - -1.6 505,258 - GFF466 0.20 -0.3 505,258 - GFF466 0.20 -0.2 505,518 + -0.8 505,518 + -0.3 505,518 + -1.1 505,519 - +0.1 505,519 - -0.1 505,519 - -0.5 505,656 + GFF467 0.20 +2.8 505,656 + GFF467 0.20 -1.2 505,657 - GFF467 0.20 -1.0 505,686 + GFF467 0.23 -2.5 505,687 - GFF467 0.24 -0.8 505,687 - GFF467 0.24 -0.7 505,803 + GFF467 0.39 -0.0 505,803 + GFF467 0.39 +0.2 505,803 + GFF467 0.39 +0.3 505,803 + GFF467 0.39 -0.3 505,812 + GFF467 0.40 +0.8 505,812 + GFF467 0.40 +0.2 505,812 + GFF467 0.40 +0.1 505,812 + GFF467 0.40 +0.4 505,813 - GFF467 0.40 -0.4 505,813 - GFF467 0.40 -1.1 505,813 - GFF467 0.40 -0.7 505,825 - GFF467 0.41 +0.2 505,942 - GFF467 0.56 +0.2 505,942 - GFF467 0.56 -1.8
Or see this region's nucleotide sequence