Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4622 and GFF4623 are separated by 251 nucleotides GFF4623 and GFF4624 are separated by 77 nucleotides GFF4624 and GFF4625 are separated by 107 nucleotides
GFF4622 - Permease of the drug/metabolite transporter (DMT) superfamily, at 264,094 to 264,915
GFF4622
GFF4623 - Transcriptional regulator, AcrR family, at 265,167 to 265,817
GFF4623
GFF4624 - N-acetylglutamate kinase (EC 2.7.2.8), at 265,895 to 266,788
GFF4624
GFF4625 - Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.33), at 266,896 to 267,993
GFF4625
Position (kb) 265
266
267 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.924 kb on + strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 264.925 kb on - strand at 265.067 kb on + strand at 265.115 kb on + strand at 265.115 kb on + strand at 265.115 kb on + strand at 265.115 kb on + strand at 265.115 kb on + strand at 265.115 kb on + strand at 265.115 kb on + strand at 265.116 kb on - strand at 265.612 kb on + strand, within GFF4623 at 265.612 kb on + strand, within GFF4623 at 265.613 kb on - strand, within GFF4623 at 265.782 kb on + strand at 265.783 kb on - strand at 265.783 kb on - strand at 265.837 kb on + strand at 265.837 kb on + strand at 266.942 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.955 kb on - strand at 266.955 kb on - strand at 266.955 kb on - strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 267.053 kb on + strand, within GFF4625 at 267.054 kb on - strand, within GFF4625 at 267.054 kb on - strand, within GFF4625 at 267.197 kb on + strand, within GFF4625 at 267.198 kb on - strand, within GFF4625 at 267.198 kb on - strand, within GFF4625 at 267.198 kb on - strand, within GFF4625 at 267.228 kb on - strand, within GFF4625 at 267.320 kb on + strand, within GFF4625 at 267.320 kb on + strand, within GFF4625 at 267.321 kb on - strand, within GFF4625 at 267.321 kb on - strand, within GFF4625 at 267.359 kb on + strand, within GFF4625 at 267.360 kb on - strand, within GFF4625 at 267.497 kb on + strand, within GFF4625
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 264,924 + +0.5 264,924 + -0.9 264,924 + +0.6 264,924 + +0.2 264,924 + -0.7 264,924 + -0.2 264,925 - -0.2 264,925 - -0.4 264,925 - -0.2 264,925 - -1.7 264,925 - +0.1 264,925 - +0.6 264,925 - -0.1 264,925 - +0.9 264,925 - -0.1 265,067 + -0.1 265,115 + +0.3 265,115 + -0.6 265,115 + -0.8 265,115 + +0.2 265,115 + +1.1 265,115 + +1.6 265,115 + -0.0 265,116 - +0.3 265,612 + GFF4623 0.68 +0.1 265,612 + GFF4623 0.68 -0.8 265,613 - GFF4623 0.69 -0.3 265,782 + -0.4 265,783 - -0.4 265,783 - +1.2 265,837 + -0.3 265,837 + -0.0 266,942 + -2.6 266,954 + -4.2 266,954 + -3.0 266,954 + -0.9 266,954 + -2.2 266,954 + -2.1 266,954 + -2.3 266,954 + -2.9 266,954 + -4.1 266,955 - -1.7 266,955 - -1.8 266,955 - -0.9 266,993 + -0.7 266,993 + -2.3 266,993 + -3.2 266,993 + -2.9 266,993 + -2.9 266,993 + -2.1 266,993 + -3.5 266,993 + -1.7 266,994 - -1.7 266,994 - -2.8 266,994 - -1.5 266,994 - -2.4 266,994 - -4.6 266,994 - -1.8 266,994 - -2.2 266,994 - -0.9 267,053 + GFF4625 0.14 -0.9 267,054 - GFF4625 0.14 -0.9 267,054 - GFF4625 0.14 -1.7 267,197 + GFF4625 0.27 -4.8 267,198 - GFF4625 0.28 -3.6 267,198 - GFF4625 0.28 -4.2 267,198 - GFF4625 0.28 -0.7 267,228 - GFF4625 0.30 -0.9 267,320 + GFF4625 0.39 -2.5 267,320 + GFF4625 0.39 -2.2 267,321 - GFF4625 0.39 -1.4 267,321 - GFF4625 0.39 -1.2 267,359 + GFF4625 0.42 -3.3 267,360 - GFF4625 0.42 -2.4 267,497 + GFF4625 0.55 -3.4
Or see this region's nucleotide sequence