Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4614 and GFF4615 are separated by 27 nucleotides GFF4615 and GFF4616 are separated by 26 nucleotides GFF4616 and GFF4617 overlap by 4 nucleotides GFF4617 and GFF4618 are separated by 110 nucleotides
GFF4614 - Transcriptional regulator, AraC family, at 256,436 to 257,287
GFF4614
GFF4615 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, at 257,315 to 258,163
GFF4615
GFF4616 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 258,190 to 258,936
GFF4616
GFF4617 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 258,933 to 259,667
GFF4617
GFF4618 - Hexuronate utilization operon transcriptional repressor ExuR, at 259,778 to 260,485
GFF4618
Position (kb) 258
259 Strain fitness (log2 ratio) -1
0
1 at 257.196 kb on + strand, within GFF4614 at 257.196 kb on + strand, within GFF4614 at 257.487 kb on + strand, within GFF4615 at 257.488 kb on - strand, within GFF4615 at 257.488 kb on - strand, within GFF4615 at 257.716 kb on - strand, within GFF4615 at 257.728 kb on - strand, within GFF4615 at 257.754 kb on + strand, within GFF4615 at 257.755 kb on - strand, within GFF4615 at 257.755 kb on - strand, within GFF4615 at 257.925 kb on + strand, within GFF4615 at 257.925 kb on + strand, within GFF4615 at 257.925 kb on + strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 258.194 kb on + strand at 258.194 kb on + strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.537 kb on - strand, within GFF4616 at 258.936 kb on + strand at 258.937 kb on - strand at 258.937 kb on - strand at 259.177 kb on + strand, within GFF4617 at 259.177 kb on + strand, within GFF4617 at 259.178 kb on - strand, within GFF4617 at 259.534 kb on + strand, within GFF4617 at 259.535 kb on - strand, within GFF4617 at 259.711 kb on - strand at 259.711 kb on - strand at 259.715 kb on + strand at 259.715 kb on + strand at 259.715 kb on - strand at 259.716 kb on - strand at 259.716 kb on - strand at 259.716 kb on - strand at 259.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 257,196 + GFF4614 0.89 -0.0 257,196 + GFF4614 0.89 -0.4 257,487 + GFF4615 0.20 -0.7 257,488 - GFF4615 0.20 -1.4 257,488 - GFF4615 0.20 +1.1 257,716 - GFF4615 0.47 -0.9 257,728 - GFF4615 0.49 -1.5 257,754 + GFF4615 0.52 +0.7 257,755 - GFF4615 0.52 -0.1 257,755 - GFF4615 0.52 -0.2 257,925 + GFF4615 0.72 -1.0 257,925 + GFF4615 0.72 -0.7 257,925 + GFF4615 0.72 +0.4 257,926 - GFF4615 0.72 -1.0 257,926 - GFF4615 0.72 +0.1 257,926 - GFF4615 0.72 -1.2 257,926 - GFF4615 0.72 +0.0 258,194 + +1.1 258,194 + +0.4 258,195 - +0.0 258,195 - -1.1 258,195 - +0.4 258,195 - -0.4 258,195 - -0.7 258,195 - -1.3 258,195 - -0.5 258,195 - -0.3 258,195 - -0.4 258,195 - -0.5 258,537 - GFF4616 0.46 -0.4 258,936 + +0.8 258,937 - +0.5 258,937 - +0.2 259,177 + GFF4617 0.33 -0.9 259,177 + GFF4617 0.33 +0.2 259,178 - GFF4617 0.33 -0.9 259,534 + GFF4617 0.82 +0.4 259,535 - GFF4617 0.82 +0.2 259,711 - +0.5 259,711 - +0.3 259,715 + +0.1 259,715 + -0.4 259,715 - -0.4 259,716 - -0.2 259,716 - -0.0 259,716 - +0.3 259,732 - -0.2
Or see this region's nucleotide sequence