Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4541 and GFF4542 are separated by 97 nucleotides GFF4542 and GFF4543 overlap by 4 nucleotides GFF4543 and GFF4544 overlap by 4 nucleotides GFF4544 and GFF4545 are separated by 161 nucleotides
GFF4541 - Uncharacterized protein conserved in bacteria, at 179,393 to 180,772
GFF4541
GFF4542 - hypothetical protein, at 180,870 to 181,364
GFF4542
GFF4543 - no description, at 181,361 to 181,795
GFF4543
GFF4544 - RNA polymerase sigma factor RpoE, at 181,792 to 182,292
GFF4544
GFF4545 - Transcriptional regulator, AraC family, at 182,454 to 183,251
GFF4545
Position (kb) 181
182 Strain fitness (log2 ratio) -2
-1
0
1
2 at 180.361 kb on - strand, within GFF4541 at 180.361 kb on - strand, within GFF4541 at 180.498 kb on + strand, within GFF4541 at 180.498 kb on + strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.499 kb on - strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.588 kb on + strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.589 kb on - strand, within GFF4541 at 180.871 kb on + strand at 180.871 kb on + strand at 180.872 kb on - strand at 180.872 kb on - strand at 181.384 kb on - strand at 181.758 kb on + strand at 181.758 kb on + strand at 181.759 kb on - strand at 181.759 kb on - strand at 181.759 kb on - strand at 181.803 kb on - strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.810 kb on + strand at 181.811 kb on - strand at 181.811 kb on - strand at 181.811 kb on - strand at 181.811 kb on - strand at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.925 kb on + strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.926 kb on - strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.928 kb on + strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 181.929 kb on - strand, within GFF4544 at 182.102 kb on + strand, within GFF4544 at 182.103 kb on - strand, within GFF4544 at 182.103 kb on - strand, within GFF4544 at 182.103 kb on - strand, within GFF4544 at 182.327 kb on - strand at 182.408 kb on + strand at 182.408 kb on + strand at 182.408 kb on + strand at 182.485 kb on + strand at 182.548 kb on + strand, within GFF4545 at 182.548 kb on + strand, within GFF4545 at 182.549 kb on - strand, within GFF4545 at 182.549 kb on - strand, within GFF4545
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 180,361 - GFF4541 0.70 +1.2 180,361 - GFF4541 0.70 -0.5 180,498 + GFF4541 0.80 +1.0 180,498 + GFF4541 0.80 -1.3 180,499 - GFF4541 0.80 -0.3 180,499 - GFF4541 0.80 -0.0 180,499 - GFF4541 0.80 -0.2 180,499 - GFF4541 0.80 +0.2 180,588 + GFF4541 0.87 -0.5 180,588 + GFF4541 0.87 -1.4 180,588 + GFF4541 0.87 +1.2 180,588 + GFF4541 0.87 +0.3 180,588 + GFF4541 0.87 +0.4 180,589 - GFF4541 0.87 -0.4 180,589 - GFF4541 0.87 -0.3 180,589 - GFF4541 0.87 -1.1 180,589 - GFF4541 0.87 +0.6 180,589 - GFF4541 0.87 -0.5 180,589 - GFF4541 0.87 -0.2 180,589 - GFF4541 0.87 +0.8 180,589 - GFF4541 0.87 +0.5 180,589 - GFF4541 0.87 -0.7 180,589 - GFF4541 0.87 -0.8 180,589 - GFF4541 0.87 -0.3 180,589 - GFF4541 0.87 +0.4 180,871 + +0.3 180,871 + +0.2 180,872 - +0.1 180,872 - -0.2 181,384 - -0.1 181,758 + +0.2 181,758 + +0.6 181,759 - -0.2 181,759 - -0.3 181,759 - -1.4 181,803 - -0.1 181,810 + +0.7 181,810 + +1.6 181,810 + -0.8 181,810 + +0.2 181,810 + -0.4 181,810 + -0.6 181,811 - +0.6 181,811 - +0.4 181,811 - -0.1 181,811 - -0.7 181,925 + GFF4544 0.27 -0.0 181,925 + GFF4544 0.27 -0.6 181,925 + GFF4544 0.27 -1.0 181,925 + GFF4544 0.27 -0.3 181,925 + GFF4544 0.27 -0.5 181,925 + GFF4544 0.27 +0.2 181,926 - GFF4544 0.27 +0.4 181,926 - GFF4544 0.27 +0.1 181,926 - GFF4544 0.27 -0.2 181,926 - GFF4544 0.27 +0.5 181,928 + GFF4544 0.27 +1.6 181,928 + GFF4544 0.27 -0.4 181,928 + GFF4544 0.27 +2.0 181,928 + GFF4544 0.27 -0.2 181,928 + GFF4544 0.27 -1.2 181,928 + GFF4544 0.27 -1.3 181,928 + GFF4544 0.27 -1.0 181,929 - GFF4544 0.27 +0.8 181,929 - GFF4544 0.27 -2.4 181,929 - GFF4544 0.27 +0.2 181,929 - GFF4544 0.27 +1.8 181,929 - GFF4544 0.27 +0.0 181,929 - GFF4544 0.27 -0.5 182,102 + GFF4544 0.62 +0.5 182,103 - GFF4544 0.62 -0.5 182,103 - GFF4544 0.62 +1.9 182,103 - GFF4544 0.62 +0.3 182,327 - +0.5 182,408 + -0.1 182,408 + -0.7 182,408 + +0.2 182,485 + +0.1 182,548 + GFF4545 0.12 -0.9 182,548 + GFF4545 0.12 -0.2 182,549 - GFF4545 0.12 +0.2 182,549 - GFF4545 0.12 -1.8
Or see this region's nucleotide sequence