Strain Fitness in Variovorax sp. SCN45 around GFF4472

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4470 and GFF4471 are separated by 2 nucleotidesGFF4471 and GFF4472 overlap by 4 nucleotidesGFF4472 and GFF4473 overlap by 4 nucleotides GFF4470 - Cell division protein FtsQ, at 110,768 to 111,556 GFF4470 GFF4471 - D-alanine--D-alanine ligase (EC 6.3.2.4), at 111,559 to 112,530 GFF4471 GFF4472 - UDP-N-acetylmuramate--L-alanine ligase (EC 6.3.2.8), at 112,527 to 113,996 GFF4472 GFF4473 - UDP-N-acetylglucosamine--N-acetylmuramyl-(pentape ptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227), at 113,993 to 115,057 GFF4473 Position (kb) 112 113 114Strain fitness (log2 ratio) -1 0 1 2at 111.560 kb on + strandat 111.560 kb on + strandat 111.560 kb on + strandat 111.560 kb on + strandat 111.560 kb on + strandat 111.560 kb on + strandat 111.560 kb on + strandat 111.560 kb on + strandat 111.561 kb on - strandat 111.561 kb on - strandat 111.561 kb on - strandat 111.561 kb on - strandat 111.563 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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111,560 + -1.2
111,560 + -0.5
111,560 + +0.0
111,560 + +0.3
111,560 + -0.0
111,560 + -0.4
111,560 + -0.7
111,560 + -0.4
111,561 - +0.1
111,561 - -0.5
111,561 - -0.4
111,561 - -0.5
111,563 - +2.1

Or see this region's nucleotide sequence