Strain Fitness in Variovorax sp. SCN45 around GFF4367

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4365 and GFF4366 overlap by 4 nucleotidesGFF4366 and GFF4367 are separated by 7 nucleotidesGFF4367 and GFF4368 are separated by 35 nucleotides GFF4365 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 287,788 to 289,794 GFF4365 GFF4366 - BUG/TctC family periplasmic protein, at 289,791 to 290,777 GFF4366 GFF4367 - KEGG: regulator of ribonuclease activity A, at 290,785 to 291,270 GFF4367 GFF4368 - Peptidoglycan D,D-transpeptidase MrdA (EC 3.4.16.4), at 291,306 to 293,294 GFF4368 Position (kb) 290 291 292Strain fitness (log2 ratio) -1 0 1at 290.115 kb on + strand, within GFF4366at 290.115 kb on + strand, within GFF4366at 290.116 kb on - strand, within GFF4366at 290.238 kb on + strand, within GFF4366at 290.268 kb on + strand, within GFF4366at 290.269 kb on - strand, within GFF4366at 290.498 kb on - strand, within GFF4366at 290.541 kb on + strand, within GFF4366at 291.288 kb on - strandat 292.135 kb on + strand, within GFF4368at 292.136 kb on - strand, within GFF4368at 292.136 kb on - strand, within GFF4368at 292.136 kb on - strand, within GFF4368at 292.226 kb on - strand, within GFF4368

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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290,115 + GFF4366 0.33 +1.6
290,115 + GFF4366 0.33 +0.0
290,116 - GFF4366 0.33 +0.2
290,238 + GFF4366 0.45 -0.1
290,268 + GFF4366 0.48 +0.6
290,269 - GFF4366 0.48 -0.6
290,498 - GFF4366 0.72 -1.5
290,541 + GFF4366 0.76 +0.6
291,288 - +0.4
292,135 + GFF4368 0.42 +0.7
292,136 - GFF4368 0.42 -0.0
292,136 - GFF4368 0.42 +1.0
292,136 - GFF4368 0.42 +1.3
292,226 - GFF4368 0.46 +0.9

Or see this region's nucleotide sequence