Strain Fitness in Variovorax sp. SCN45 around GFF4349

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4348 and GFF4349 overlap by 14 nucleotidesGFF4349 and GFF4350 overlap by 4 nucleotidesGFF4350 and GFF4351 are separated by 18 nucleotides GFF4348 - hypothetical protein, at 270,986 to 272,101 GFF4348 GFF4349 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 272,088 to 272,786 GFF4349 GFF4350 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 272,783 to 273,478 GFF4350 GFF4351 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 273,497 to 274,678 GFF4351 Position (kb) 272 273Strain fitness (log2 ratio) -1 0 1at 271.173 kb on - strand, within GFF4348at 271.712 kb on + strand, within GFF4348at 271.712 kb on + strand, within GFF4348at 271.784 kb on + strand, within GFF4348at 272.939 kb on - strand, within GFF4350at 273.692 kb on + strand, within GFF4351

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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271,173 - GFF4348 0.17 -0.9
271,712 + GFF4348 0.65 -0.1
271,712 + GFF4348 0.65 +0.6
271,784 + GFF4348 0.72 +0.4
272,939 - GFF4350 0.22 -1.1
273,692 + GFF4351 0.16 -0.5

Or see this region's nucleotide sequence