Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4328 and GFF4329 are separated by 151 nucleotides GFF4329 and GFF4330 are separated by 3 nucleotides GFF4330 and GFF4331 are separated by 285 nucleotides
GFF4328 - hypothetical protein, at 249,979 to 251,094
GFF4328
GFF4329 - Enoyl-CoA hydratase (EC 4.2.1.17), at 251,246 to 252,037
GFF4329
GFF4330 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 252,041 to 252,949
GFF4330
GFF4331 - Oxidoreductase, GMC family, at 253,235 to 254,977
GFF4331
Position (kb) 252
253 Strain fitness (log2 ratio) -2
-1
0
1 at 251.116 kb on + strand at 251.116 kb on + strand at 251.117 kb on - strand at 251.131 kb on + strand at 251.144 kb on + strand at 251.183 kb on - strand at 251.459 kb on + strand, within GFF4329 at 251.459 kb on + strand, within GFF4329 at 251.460 kb on - strand, within GFF4329 at 252.913 kb on + strand at 252.913 kb on + strand at 252.947 kb on + strand at 253.033 kb on + strand at 253.034 kb on - strand at 253.052 kb on - strand at 253.052 kb on - strand at 253.062 kb on + strand at 253.114 kb on + strand at 253.114 kb on + strand at 253.217 kb on - strand at 253.254 kb on + strand at 253.255 kb on - strand at 253.255 kb on - strand at 253.255 kb on - strand at 253.262 kb on + strand at 253.262 kb on + strand at 253.434 kb on - strand, within GFF4331 at 253.541 kb on + strand, within GFF4331 at 253.541 kb on + strand, within GFF4331 at 253.541 kb on + strand, within GFF4331 at 253.566 kb on - strand, within GFF4331 at 253.604 kb on + strand, within GFF4331 at 253.604 kb on + strand, within GFF4331 at 253.811 kb on + strand, within GFF4331
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 251,116 + -0.2 251,116 + +0.2 251,117 - -1.9 251,131 + +0.3 251,144 + +0.0 251,183 - -1.4 251,459 + GFF4329 0.27 +0.8 251,459 + GFF4329 0.27 +0.3 251,460 - GFF4329 0.27 -0.1 252,913 + +0.2 252,913 + +0.1 252,947 + +1.2 253,033 + -2.2 253,034 - +1.4 253,052 - -0.0 253,052 - -0.3 253,062 + -0.8 253,114 + -0.3 253,114 + +0.1 253,217 - -0.8 253,254 + -0.8 253,255 - -0.2 253,255 - +0.1 253,255 - -0.2 253,262 + -0.4 253,262 + +0.8 253,434 - GFF4331 0.11 +0.4 253,541 + GFF4331 0.18 -0.7 253,541 + GFF4331 0.18 +0.6 253,541 + GFF4331 0.18 -0.2 253,566 - GFF4331 0.19 +0.3 253,604 + GFF4331 0.21 -0.1 253,604 + GFF4331 0.21 -0.3 253,811 + GFF4331 0.33 -0.4
Or see this region's nucleotide sequence