Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4269 and GFF4270 are separated by 83 nucleotides GFF4270 and GFF4271 are separated by 62 nucleotides GFF4271 and GFF4272 are separated by 96 nucleotides
GFF4269 - Uncharacterized SAM-dependent O-methyltransferase, at 189,844 to 190,500
GFF4269
GFF4270 - Transcriptional regulator, AcrR family, at 190,584 to 191,216
GFF4270
GFF4271 - Transcriptional regulator, LysR family, at 191,279 to 192,205
GFF4271
GFF4272 - BUG/TctC family periplasmic protein, at 192,302 to 193,282
GFF4272
Position (kb)
191
192
193 Strain fitness (log2 ratio)
-1
0
1 at 190.296 kb on - strand, within GFF4269 at 190.353 kb on - strand, within GFF4269 at 190.761 kb on + strand, within GFF4270 at 190.767 kb on + strand, within GFF4270 at 190.767 kb on + strand, within GFF4270 at 190.966 kb on - strand, within GFF4270 at 191.190 kb on + strand at 191.281 kb on - strand at 191.281 kb on - strand at 191.789 kb on + strand, within GFF4271 at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.182 kb on - strand at 192.182 kb on - strand at 192.232 kb on - strand at 192.232 kb on - strand at 192.410 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.612 kb on - strand, within GFF4272 at 192.612 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 190,296 - GFF4269 0.69 -0.1 190,353 - GFF4269 0.77 +1.2 190,761 + GFF4270 0.28 -0.2 190,767 + GFF4270 0.29 -0.7 190,767 + GFF4270 0.29 -0.5 190,966 - GFF4270 0.60 +0.2 191,190 + +0.1 191,281 - -1.1 191,281 - -0.1 191,789 + GFF4271 0.55 +1.2 192,123 + -0.9 192,123 + -1.0 192,123 + -0.2 192,123 + +0.0 192,123 + -1.0 192,124 - +0.3 192,124 - +0.0 192,124 - +0.8 192,124 - -0.6 192,182 - +0.3 192,182 - -0.3 192,232 - +0.6 192,232 - +0.3 192,410 + GFF4272 0.11 +1.6 192,533 + GFF4272 0.24 -1.1 192,533 + GFF4272 0.24 -0.7 192,533 + GFF4272 0.24 +0.5 192,533 + GFF4272 0.24 -0.4 192,534 - GFF4272 0.24 +0.5 192,534 - GFF4272 0.24 -0.8 192,534 - GFF4272 0.24 +0.1 192,534 - GFF4272 0.24 +0.5 192,612 - GFF4272 0.32 -0.1 192,612 - GFF4272 0.32 -1.3 192,765 - GFF4272 0.47 -1.0 192,765 - GFF4272 0.47 -0.4 192,765 - GFF4272 0.47 +0.1 192,765 - GFF4272 0.47 -0.4 192,765 - GFF4272 0.47 +0.0 192,765 - GFF4272 0.47 +0.1
Or see this region's nucleotide sequence