Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4153 and GFF4154 overlap by 28 nucleotides GFF4154 and GFF4155 overlap by 4 nucleotides GFF4155 and GFF4156 overlap by 14 nucleotides GFF4156 and GFF4157 overlap by 4 nucleotides
GFF4153 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 59,603 to 60,544
GFF4153
GFF4154 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,517 to 61,371
GFF4154
GFF4155 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 61,368 to 62,138
GFF4155
GFF4156 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 62,125 to 62,865
GFF4156
GFF4157 - hypothetical protein, at 62,862 to 63,812
GFF4157
Position (kb) 61
62
63 Strain fitness (log2 ratio) -1
0
1 at 60.526 kb on + strand at 60.649 kb on + strand, within GFF4154 at 60.893 kb on - strand, within GFF4154 at 61.090 kb on + strand, within GFF4154 at 61.091 kb on - strand, within GFF4154 at 61.096 kb on + strand, within GFF4154 at 61.096 kb on + strand, within GFF4154 at 61.097 kb on - strand, within GFF4154 at 61.097 kb on - strand, within GFF4154 at 61.097 kb on - strand, within GFF4154 at 61.099 kb on + strand, within GFF4154 at 61.099 kb on + strand, within GFF4154 at 61.100 kb on - strand, within GFF4154 at 61.255 kb on + strand, within GFF4154 at 62.104 kb on - strand at 62.104 kb on - strand at 62.428 kb on + strand, within GFF4156 at 62.429 kb on - strand, within GFF4156 at 62.641 kb on + strand, within GFF4156 at 62.642 kb on - strand, within GFF4156 at 62.734 kb on + strand, within GFF4156 at 62.734 kb on + strand, within GFF4156 at 62.887 kb on - strand at 62.887 kb on - strand at 62.994 kb on + strand, within GFF4157 at 62.995 kb on - strand, within GFF4157 at 62.995 kb on - strand, within GFF4157
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 60,526 + +1.1 60,649 + GFF4154 0.15 +0.2 60,893 - GFF4154 0.44 +1.3 61,090 + GFF4154 0.67 -1.2 61,091 - GFF4154 0.67 +0.0 61,096 + GFF4154 0.68 -0.9 61,096 + GFF4154 0.68 +0.3 61,097 - GFF4154 0.68 -0.4 61,097 - GFF4154 0.68 +0.4 61,097 - GFF4154 0.68 -0.6 61,099 + GFF4154 0.68 +0.8 61,099 + GFF4154 0.68 -1.3 61,100 - GFF4154 0.68 -0.4 61,255 + GFF4154 0.86 +0.6 62,104 - -0.4 62,104 - -0.5 62,428 + GFF4156 0.41 +0.3 62,429 - GFF4156 0.41 +0.1 62,641 + GFF4156 0.70 +0.9 62,642 - GFF4156 0.70 +1.6 62,734 + GFF4156 0.82 -0.2 62,734 + GFF4156 0.82 +0.3 62,887 - -1.2 62,887 - -0.2 62,994 + GFF4157 0.14 -0.3 62,995 - GFF4157 0.14 -0.9 62,995 - GFF4157 0.14 -0.7
Or see this region's nucleotide sequence