Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF409 and GFF410 are separated by 14 nucleotides GFF410 and GFF411 overlap by 4 nucleotides GFF411 and GFF412 are separated by 119 nucleotides
GFF409 - Osmolarity sensory histidine kinase EnvZ, at 443,143 to 444,564
GFF409
GFF410 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 444,579 to 445,067
GFF410
GFF411 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60), at 445,064 to 445,786
GFF411
GFF412 - Transcription-repair coupling factor, at 445,906 to 449,388
GFF412
Position (kb)
445
446 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 444.097 kb on + strand, within GFF409 at 444.097 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.113 kb on - strand, within GFF409 at 444.288 kb on - strand, within GFF409 at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 445.797 kb on - strand at 445.841 kb on + strand at 445.841 kb on + strand at 445.896 kb on + strand at 446.134 kb on + strand at 446.246 kb on - strand at 446.278 kb on + strand, within GFF412 at 446.278 kb on + strand, within GFF412 at 446.278 kb on + strand, within GFF412 at 446.278 kb on + strand, within GFF412 at 446.279 kb on - strand, within GFF412 at 446.279 kb on - strand, within GFF412 at 446.387 kb on - strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.635 kb on + strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.636 kb on - strand, within GFF412 at 446.677 kb on + strand, within GFF412 at 446.678 kb on - strand, within GFF412 at 446.678 kb on - strand, within GFF412 at 446.678 kb on - strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.680 kb on + strand, within GFF412 at 446.681 kb on - strand, within GFF412 at 446.681 kb on - strand, within GFF412 at 446.681 kb on - strand, within GFF412 at 446.681 kb on - strand, within GFF412 at 446.681 kb on - strand, within GFF412 at 446.681 kb on - strand, within GFF412 at 446.681 kb on - strand, within GFF412 at 446.723 kb on - strand, within GFF412 at 446.723 kb on - strand, within GFF412 at 446.723 kb on - strand, within GFF412 at 446.723 kb on - strand, within GFF412
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 444,097 + GFF409 0.67 -0.8 444,097 + GFF409 0.67 +0.2 444,112 + GFF409 0.68 +0.4 444,112 + GFF409 0.68 -2.3 444,112 + GFF409 0.68 +0.2 444,113 - GFF409 0.68 -2.1 444,288 - GFF409 0.81 -0.8 444,491 + -0.4 444,491 + +0.2 444,491 + -1.0 444,491 + -0.8 444,492 - -0.4 444,492 - -1.8 444,492 - +0.6 444,492 - -1.7 444,492 - -0.4 444,513 + -0.4 444,513 + +1.2 444,513 + -0.1 444,514 - +0.2 444,514 - +1.8 444,514 - -0.6 444,514 - +0.4 444,514 - -0.2 444,514 - +0.7 444,514 - +0.1 445,797 - +1.4 445,841 + -0.4 445,841 + +0.0 445,896 + -0.3 446,134 + -0.5 446,246 - -0.6 446,278 + GFF412 0.11 +0.5 446,278 + GFF412 0.11 +0.4 446,278 + GFF412 0.11 -0.1 446,278 + GFF412 0.11 -0.5 446,279 - GFF412 0.11 -0.5 446,279 - GFF412 0.11 +0.4 446,387 - GFF412 0.14 -1.6 446,635 + GFF412 0.21 -0.6 446,635 + GFF412 0.21 +0.3 446,635 + GFF412 0.21 +0.2 446,635 + GFF412 0.21 +0.3 446,635 + GFF412 0.21 +1.0 446,635 + GFF412 0.21 +2.6 446,635 + GFF412 0.21 -1.6 446,635 + GFF412 0.21 +0.2 446,635 + GFF412 0.21 -0.1 446,635 + GFF412 0.21 +0.8 446,636 - GFF412 0.21 +0.1 446,636 - GFF412 0.21 -0.6 446,636 - GFF412 0.21 -0.7 446,636 - GFF412 0.21 +0.1 446,636 - GFF412 0.21 -0.1 446,636 - GFF412 0.21 +0.2 446,636 - GFF412 0.21 -0.1 446,636 - GFF412 0.21 +0.3 446,636 - GFF412 0.21 +0.6 446,636 - GFF412 0.21 -1.1 446,636 - GFF412 0.21 -0.5 446,636 - GFF412 0.21 +0.9 446,636 - GFF412 0.21 +1.2 446,677 + GFF412 0.22 -0.0 446,678 - GFF412 0.22 -1.1 446,678 - GFF412 0.22 +0.6 446,678 - GFF412 0.22 +1.4 446,680 + GFF412 0.22 -0.9 446,680 + GFF412 0.22 -0.1 446,680 + GFF412 0.22 +2.2 446,680 + GFF412 0.22 -0.6 446,680 + GFF412 0.22 +0.5 446,680 + GFF412 0.22 -1.5 446,680 + GFF412 0.22 +1.0 446,680 + GFF412 0.22 -2.3 446,681 - GFF412 0.22 -0.0 446,681 - GFF412 0.22 +0.7 446,681 - GFF412 0.22 -0.4 446,681 - GFF412 0.22 +0.3 446,681 - GFF412 0.22 +0.0 446,681 - GFF412 0.22 -0.9 446,681 - GFF412 0.22 -1.4 446,723 - GFF412 0.23 -1.3 446,723 - GFF412 0.23 -0.4 446,723 - GFF412 0.23 -0.4 446,723 - GFF412 0.23 -2.2
Or see this region's nucleotide sequence