Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4087 and GFF4088 are separated by 22 nucleotides GFF4088 and GFF4089 are separated by 57 nucleotides GFF4089 and GFF4090 overlap by 1 nucleotides
GFF4087 - Cystathionine beta-lyase (EC 4.4.1.8), at 299,698 to 300,867
GFF4087
GFF4088 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 300,890 to 301,816
GFF4088
GFF4089 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 301,874 to 302,611
GFF4089
GFF4090 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 302,611 to 303,300
GFF4090
Position (kb) 300
301
302 Strain fitness (log2 ratio) -1
0
1
2 at 300.011 kb on - strand, within GFF4087 at 300.304 kb on + strand, within GFF4087 at 300.304 kb on + strand, within GFF4087 at 300.304 kb on + strand, within GFF4087 at 300.305 kb on - strand, within GFF4087 at 300.364 kb on + strand, within GFF4087 at 300.364 kb on + strand, within GFF4087 at 300.634 kb on + strand, within GFF4087 at 301.607 kb on + strand, within GFF4088 at 301.608 kb on - strand, within GFF4088 at 301.814 kb on + strand at 301.815 kb on - strand at 301.815 kb on - strand at 301.860 kb on - strand at 301.880 kb on + strand at 302.075 kb on + strand, within GFF4089 at 302.075 kb on + strand, within GFF4089 at 302.075 kb on + strand, within GFF4089 at 302.076 kb on - strand, within GFF4089 at 302.076 kb on - strand, within GFF4089 at 302.149 kb on - strand, within GFF4089 at 302.477 kb on + strand, within GFF4089 at 302.525 kb on + strand, within GFF4089 at 302.525 kb on + strand, within GFF4089 at 302.525 kb on + strand, within GFF4089 at 302.525 kb on + strand, within GFF4089 at 302.540 kb on + strand at 302.541 kb on - strand at 302.789 kb on - strand, within GFF4090
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 300,011 - GFF4087 0.27 +0.4 300,304 + GFF4087 0.52 +1.4 300,304 + GFF4087 0.52 +0.6 300,304 + GFF4087 0.52 +0.3 300,305 - GFF4087 0.52 -0.2 300,364 + GFF4087 0.57 +2.3 300,364 + GFF4087 0.57 +0.4 300,634 + GFF4087 0.80 +0.1 301,607 + GFF4088 0.77 +0.1 301,608 - GFF4088 0.77 +1.0 301,814 + +0.5 301,815 - -1.1 301,815 - -0.4 301,860 - -0.7 301,880 + -0.7 302,075 + GFF4089 0.27 -0.9 302,075 + GFF4089 0.27 +0.4 302,075 + GFF4089 0.27 -0.2 302,076 - GFF4089 0.27 -1.2 302,076 - GFF4089 0.27 -0.4 302,149 - GFF4089 0.37 +0.0 302,477 + GFF4089 0.82 +0.3 302,525 + GFF4089 0.88 -0.2 302,525 + GFF4089 0.88 -0.4 302,525 + GFF4089 0.88 -0.2 302,525 + GFF4089 0.88 -0.6 302,540 + +0.4 302,541 - -1.3 302,789 - GFF4090 0.26 +0.9
Or see this region's nucleotide sequence