Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4074 and GFF4075 are separated by 46 nucleotides GFF4075 and GFF4076 are separated by 75 nucleotides GFF4076 and GFF4077 are separated by 49 nucleotides GFF4077 and GFF4078 are separated by 82 nucleotides
GFF4074 - Multicopper oxidase, at 288,150 to 289,565
GFF4074
GFF4075 - Copper tolerance protein, at 289,612 to 290,103
GFF4075
GFF4076 - hypothetical protein, at 290,179 to 290,553
GFF4076
GFF4077 - hypothetical protein, at 290,603 to 291,415
GFF4077
GFF4078 - hypothetical protein, at 291,498 to 291,890
GFF4078
Position (kb) 290
291 Strain fitness (log2 ratio) -2
-1
0
1
2 at 289.218 kb on + strand, within GFF4074 at 289.218 kb on + strand, within GFF4074 at 289.219 kb on - strand, within GFF4074 at 289.219 kb on - strand, within GFF4074 at 289.219 kb on - strand, within GFF4074 at 289.219 kb on - strand, within GFF4074 at 289.383 kb on + strand, within GFF4074 at 289.525 kb on + strand at 289.526 kb on - strand at 289.913 kb on - strand, within GFF4075 at 290.609 kb on + strand at 290.609 kb on + strand at 290.737 kb on + strand, within GFF4077 at 290.738 kb on - strand, within GFF4077 at 290.934 kb on - strand, within GFF4077 at 291.203 kb on + strand, within GFF4077 at 291.218 kb on + strand, within GFF4077 at 291.219 kb on - strand, within GFF4077 at 291.269 kb on + strand, within GFF4077 at 291.311 kb on + strand, within GFF4077 at 291.353 kb on + strand at 291.402 kb on - strand at 291.470 kb on + strand at 291.471 kb on - strand at 291.471 kb on - strand at 291.504 kb on + strand at 291.504 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 289,218 + GFF4074 0.75 +1.0 289,218 + GFF4074 0.75 -0.9 289,219 - GFF4074 0.75 -0.3 289,219 - GFF4074 0.75 -0.2 289,219 - GFF4074 0.75 -0.5 289,219 - GFF4074 0.75 +0.8 289,383 + GFF4074 0.87 -0.0 289,525 + -0.2 289,526 - -0.5 289,913 - GFF4075 0.61 +0.0 290,609 + +2.5 290,609 + +0.1 290,737 + GFF4077 0.16 -2.2 290,738 - GFF4077 0.17 -0.8 290,934 - GFF4077 0.41 +1.1 291,203 + GFF4077 0.74 -0.5 291,218 + GFF4077 0.76 -0.6 291,219 - GFF4077 0.76 +0.1 291,269 + GFF4077 0.82 +0.3 291,311 + GFF4077 0.87 +0.3 291,353 + +0.5 291,402 - -0.2 291,470 + +0.5 291,471 - -0.5 291,471 - -1.6 291,504 + -0.5 291,504 + -0.1
Or see this region's nucleotide sequence