Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4050 and GFF4051 are separated by 627 nucleotides GFF4051 and GFF4052 are separated by 42 nucleotides GFF4052 and GFF4053 are separated by 288 nucleotides GFF4053 and GFF4054 are separated by 110 nucleotides
GFF4050 - no description, at 263,371 to 263,559
GFF4050
GFF4051 - no description, at 264,187 to 264,441
GFF4051
GFF4052 - Putative regulatory protein clustered with metal efflux genes, P-II family, at 264,484 to 264,813
GFF4052
GFF4053 - CzcABC family efflux RND transporter, membrane fusion protein, at 265,102 to 265,560
GFF4053
GFF4054 - no description, at 265,671 to 265,964
GFF4054
Position (kb) 264
265 Strain fitness (log2 ratio) -1
0
1 at 263.498 kb on + strand, within GFF4050 at 263.557 kb on + strand at 263.582 kb on + strand at 263.586 kb on + strand at 263.586 kb on + strand at 263.586 kb on + strand at 263.586 kb on + strand at 263.586 kb on + strand at 263.587 kb on - strand at 263.587 kb on - strand at 263.587 kb on - strand at 263.587 kb on - strand at 263.587 kb on - strand at 263.664 kb on - strand at 263.664 kb on - strand at 263.743 kb on + strand at 263.818 kb on + strand at 263.819 kb on - strand at 263.832 kb on + strand at 264.010 kb on + strand at 264.465 kb on + strand at 264.692 kb on + strand, within GFF4052 at 264.801 kb on - strand at 264.801 kb on - strand at 265.103 kb on + strand at 265.103 kb on + strand at 265.104 kb on - strand at 265.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 263,498 + GFF4050 0.67 +1.5 263,557 + +0.4 263,582 + -0.3 263,586 + -0.9 263,586 + -0.3 263,586 + -0.1 263,586 + -0.2 263,586 + -0.9 263,587 - -1.3 263,587 - -0.2 263,587 - +0.5 263,587 - +0.1 263,587 - -0.3 263,664 - -0.5 263,664 - -0.1 263,743 + -0.3 263,818 + +0.8 263,819 - -0.3 263,832 + -0.5 264,010 + -0.3 264,465 + +0.4 264,692 + GFF4052 0.63 -1.5 264,801 - -1.6 264,801 - +0.1 265,103 + -0.1 265,103 + -0.0 265,104 - +0.5 265,673 - -0.6
Or see this region's nucleotide sequence