Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3946 and GFF3947 are separated by 19 nucleotides GFF3947 and GFF3948 are separated by 7 nucleotides GFF3948 and GFF3949 are separated by 180 nucleotides GFF3949 and GFF3950 are separated by 16 nucleotides
GFF3946 - Probable low-affinity inorganic phosphate transporter, at 149,552 to 150,562
GFF3946
GFF3947 - Putative transmembrane protein, at 150,582 to 151,028
GFF3947
GFF3948 - Acetyltransferase, GNAT family, at 151,036 to 151,560
GFF3948
GFF3949 - SSU ribosomal protein S16p, at 151,741 to 152,004
GFF3949
GFF3950 - 16S rRNA processing protein RimM, at 152,021 to 152,575
GFF3950
Position (kb) 150
151
152 Strain fitness (log2 ratio) -2
-1
0
1
2 at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.586 kb on + strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.587 kb on - strand at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.856 kb on - strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.858 kb on + strand, within GFF3946 at 149.859 kb on - strand, within GFF3946 at 149.859 kb on - strand, within GFF3946 at 149.859 kb on - strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.110 kb on + strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.111 kb on - strand, within GFF3946 at 150.185 kb on + strand, within GFF3946 at 150.185 kb on + strand, within GFF3946 at 150.186 kb on - strand, within GFF3946 at 150.186 kb on - strand, within GFF3946 at 150.186 kb on - strand, within GFF3946 at 150.236 kb on + strand, within GFF3946 at 150.409 kb on + strand, within GFF3946 at 150.536 kb on + strand at 150.536 kb on + strand at 150.536 kb on + strand at 150.537 kb on - strand at 150.625 kb on + strand at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.874 kb on + strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.875 kb on - strand, within GFF3947 at 150.898 kb on + strand, within GFF3947 at 150.898 kb on + strand, within GFF3947 at 150.899 kb on - strand, within GFF3947 at 150.899 kb on - strand, within GFF3947 at 150.899 kb on - strand, within GFF3947 at 150.961 kb on + strand, within GFF3947 at 150.961 kb on + strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 150.962 kb on - strand, within GFF3947 at 151.083 kb on - strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.084 kb on + strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.085 kb on - strand at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.298 kb on + strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.299 kb on - strand, within GFF3948 at 151.325 kb on + strand, within GFF3948 at 151.326 kb on - strand, within GFF3948 at 151.397 kb on + strand, within GFF3948 at 151.397 kb on + strand, within GFF3948 at 151.397 kb on + strand, within GFF3948 at 151.398 kb on - strand, within GFF3948 at 151.398 kb on - strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.502 kb on + strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.503 kb on - strand, within GFF3948 at 151.615 kb on + strand at 151.615 kb on + strand at 151.615 kb on + strand at 151.615 kb on + strand at 151.616 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 149,586 + -1.1 149,586 + -0.3 149,586 + -0.6 149,586 + +1.2 149,586 + -0.7 149,586 + -0.2 149,586 + -0.8 149,587 - -0.2 149,587 - -0.1 149,587 - +0.0 149,587 - -0.1 149,587 - +0.3 149,856 - GFF3946 0.30 +1.0 149,856 - GFF3946 0.30 +0.2 149,856 - GFF3946 0.30 -2.1 149,856 - GFF3946 0.30 +0.1 149,856 - GFF3946 0.30 -0.8 149,858 + GFF3946 0.30 -1.0 149,858 + GFF3946 0.30 -0.1 149,858 + GFF3946 0.30 +0.4 149,858 + GFF3946 0.30 -1.0 149,858 + GFF3946 0.30 -0.0 149,858 + GFF3946 0.30 -0.1 149,858 + GFF3946 0.30 -0.3 149,859 - GFF3946 0.30 +0.5 149,859 - GFF3946 0.30 +0.1 149,859 - GFF3946 0.30 -0.5 150,110 + GFF3946 0.55 -0.9 150,110 + GFF3946 0.55 -0.3 150,110 + GFF3946 0.55 -0.2 150,110 + GFF3946 0.55 -0.0 150,111 - GFF3946 0.55 +0.1 150,111 - GFF3946 0.55 -1.0 150,111 - GFF3946 0.55 -0.5 150,111 - GFF3946 0.55 -0.4 150,185 + GFF3946 0.63 -0.6 150,185 + GFF3946 0.63 +1.5 150,186 - GFF3946 0.63 -0.0 150,186 - GFF3946 0.63 -0.4 150,186 - GFF3946 0.63 -0.4 150,236 + GFF3946 0.68 -0.8 150,409 + GFF3946 0.85 -1.2 150,536 + -1.5 150,536 + +1.2 150,536 + -0.2 150,537 - -1.4 150,625 + -0.2 150,874 + GFF3947 0.65 -0.3 150,874 + GFF3947 0.65 -0.2 150,874 + GFF3947 0.65 +0.2 150,874 + GFF3947 0.65 +0.2 150,874 + GFF3947 0.65 -0.1 150,875 - GFF3947 0.66 +0.4 150,875 - GFF3947 0.66 +1.5 150,875 - GFF3947 0.66 -0.0 150,875 - GFF3947 0.66 +0.4 150,875 - GFF3947 0.66 -0.6 150,875 - GFF3947 0.66 -0.5 150,898 + GFF3947 0.71 -1.0 150,898 + GFF3947 0.71 +0.1 150,899 - GFF3947 0.71 -0.5 150,899 - GFF3947 0.71 -0.6 150,899 - GFF3947 0.71 +1.5 150,961 + GFF3947 0.85 +0.8 150,961 + GFF3947 0.85 -0.3 150,962 - GFF3947 0.85 -0.0 150,962 - GFF3947 0.85 +0.0 150,962 - GFF3947 0.85 +0.7 150,962 - GFF3947 0.85 -1.3 150,962 - GFF3947 0.85 +0.5 151,083 - +0.2 151,084 + -0.0 151,084 + +0.3 151,084 + +1.2 151,084 + +1.5 151,084 + -0.5 151,085 - -1.9 151,085 - -0.2 151,085 - +0.1 151,085 - -0.2 151,085 - -0.5 151,085 - -0.9 151,085 - -0.7 151,085 - +0.3 151,298 + GFF3948 0.50 -0.0 151,298 + GFF3948 0.50 +0.1 151,298 + GFF3948 0.50 -1.3 151,298 + GFF3948 0.50 +0.3 151,298 + GFF3948 0.50 -1.0 151,298 + GFF3948 0.50 +1.0 151,298 + GFF3948 0.50 -0.1 151,298 + GFF3948 0.50 -0.3 151,298 + GFF3948 0.50 +0.5 151,298 + GFF3948 0.50 -0.1 151,298 + GFF3948 0.50 +0.7 151,298 + GFF3948 0.50 +0.5 151,298 + GFF3948 0.50 +1.0 151,298 + GFF3948 0.50 +0.5 151,298 + GFF3948 0.50 +0.7 151,298 + GFF3948 0.50 -1.3 151,298 + GFF3948 0.50 +0.0 151,298 + GFF3948 0.50 -0.0 151,298 + GFF3948 0.50 -0.5 151,299 - GFF3948 0.50 -1.2 151,299 - GFF3948 0.50 -0.5 151,299 - GFF3948 0.50 -0.9 151,299 - GFF3948 0.50 +0.5 151,299 - GFF3948 0.50 -0.1 151,299 - GFF3948 0.50 -1.0 151,299 - GFF3948 0.50 -0.7 151,299 - GFF3948 0.50 +0.6 151,299 - GFF3948 0.50 -0.3 151,299 - GFF3948 0.50 +0.0 151,299 - GFF3948 0.50 -2.4 151,299 - GFF3948 0.50 -0.8 151,299 - GFF3948 0.50 +2.0 151,299 - GFF3948 0.50 -0.4 151,299 - GFF3948 0.50 -0.2 151,299 - GFF3948 0.50 +1.0 151,325 + GFF3948 0.55 -0.2 151,326 - GFF3948 0.55 -0.9 151,397 + GFF3948 0.69 +0.4 151,397 + GFF3948 0.69 -0.5 151,397 + GFF3948 0.69 +0.1 151,398 - GFF3948 0.69 -0.0 151,398 - GFF3948 0.69 -0.6 151,502 + GFF3948 0.89 -0.3 151,502 + GFF3948 0.89 -0.7 151,502 + GFF3948 0.89 +0.0 151,502 + GFF3948 0.89 -0.8 151,502 + GFF3948 0.89 +0.1 151,502 + GFF3948 0.89 -0.9 151,502 + GFF3948 0.89 +0.2 151,502 + GFF3948 0.89 -0.2 151,503 - GFF3948 0.89 +0.4 151,503 - GFF3948 0.89 -0.2 151,503 - GFF3948 0.89 -0.4 151,503 - GFF3948 0.89 +0.1 151,503 - GFF3948 0.89 -0.6 151,503 - GFF3948 0.89 -0.5 151,503 - GFF3948 0.89 -0.0 151,503 - GFF3948 0.89 -0.4 151,503 - GFF3948 0.89 -0.3 151,503 - GFF3948 0.89 +0.5 151,503 - GFF3948 0.89 -0.4 151,503 - GFF3948 0.89 -1.0 151,615 + -0.5 151,615 + +2.0 151,615 + -0.7 151,615 + +0.2 151,616 - -0.3
Or see this region's nucleotide sequence