Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3927 and GFF3928 are separated by 161 nucleotides GFF3928 and GFF3929 are separated by 96 nucleotides GFF3929 and GFF3930 are separated by 15 nucleotides GFF3930 and GFF3931 are separated by 77 nucleotides GFF3931 and GFF3932 are separated by 1 nucleotides
GFF3927 - N-acetylornithine aminotransferase (EC 2.6.1.11), at 131,959 to 133,167
GFF3927
GFF3928 - Histone acetyltransferase HPA2 and related acetyltransferases, at 133,329 to 133,739
GFF3928
GFF3929 - SSU ribosomal protein S20p, at 133,836 to 134,150
GFF3929
GFF3930 - Peptidoglycan lipid II flippase MurJ, at 134,166 to 135,833
GFF3930
GFF3931 - Protein sirB1, at 135,911 to 136,762
GFF3931
GFF3932 - Acetyltransferase, GNAT family, at 136,764 to 137,192
GFF3932
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-2
-1
0
1 at 133.183 kb on - strand at 133.183 kb on - strand at 133.198 kb on + strand at 133.199 kb on - strand at 133.199 kb on - strand at 133.199 kb on - strand at 133.199 kb on - strand at 133.238 kb on - strand at 133.279 kb on + strand at 133.280 kb on - strand at 133.467 kb on + strand, within GFF3928 at 133.468 kb on - strand, within GFF3928 at 133.468 kb on - strand, within GFF3928 at 133.468 kb on - strand, within GFF3928 at 133.477 kb on - strand, within GFF3928 at 133.477 kb on - strand, within GFF3928 at 133.477 kb on - strand, within GFF3928 at 133.764 kb on + strand at 134.200 kb on + strand at 134.201 kb on - strand at 134.201 kb on - strand at 134.266 kb on + strand at 134.266 kb on + strand at 134.266 kb on + strand at 134.266 kb on + strand at 134.267 kb on - strand at 134.267 kb on - strand at 134.267 kb on - strand at 134.267 kb on - strand at 135.900 kb on + strand at 135.900 kb on + strand at 135.900 kb on + strand at 135.901 kb on - strand at 135.901 kb on - strand at 135.912 kb on - strand at 135.935 kb on - strand at 135.935 kb on - strand at 135.947 kb on + strand at 135.947 kb on + strand at 135.948 kb on - strand at 135.948 kb on - strand at 136.022 kb on + strand, within GFF3931 at 136.022 kb on + strand, within GFF3931 at 136.022 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.181 kb on + strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.182 kb on - strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.184 kb on + strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.185 kb on - strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.202 kb on + strand, within GFF3931 at 136.203 kb on - strand, within GFF3931 at 136.203 kb on - strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.418 kb on + strand, within GFF3931 at 136.419 kb on - strand, within GFF3931 at 136.419 kb on - strand, within GFF3931 at 136.419 kb on - strand, within GFF3931 at 136.469 kb on + strand, within GFF3931 at 136.469 kb on + strand, within GFF3931 at 136.469 kb on + strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.470 kb on - strand, within GFF3931 at 136.523 kb on - strand, within GFF3931 at 136.682 kb on + strand at 136.682 kb on + strand at 136.682 kb on + strand at 136.683 kb on - strand at 136.683 kb on - strand at 136.683 kb on - strand at 136.683 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 133,183 - -0.8 133,183 - +0.4 133,198 + -0.8 133,199 - -0.1 133,199 - -0.2 133,199 - -0.1 133,199 - -0.3 133,238 - -0.6 133,279 + -0.8 133,280 - -0.2 133,467 + GFF3928 0.34 -0.0 133,468 - GFF3928 0.34 -2.2 133,468 - GFF3928 0.34 -0.8 133,468 - GFF3928 0.34 -0.8 133,477 - GFF3928 0.36 -0.5 133,477 - GFF3928 0.36 +0.1 133,477 - GFF3928 0.36 -1.5 133,764 + +0.6 134,200 + -0.8 134,201 - +0.3 134,201 - -0.5 134,266 + -0.9 134,266 + -1.3 134,266 + -1.3 134,266 + -1.1 134,267 - -0.1 134,267 - +0.5 134,267 - +0.0 134,267 - -1.5 135,900 + -0.7 135,900 + +0.5 135,900 + -0.1 135,901 - -1.0 135,901 - -1.8 135,912 - +0.3 135,935 - +0.2 135,935 - -0.3 135,947 + -0.5 135,947 + -0.2 135,948 - -0.0 135,948 - -1.3 136,022 + GFF3931 0.13 -0.6 136,022 + GFF3931 0.13 -0.6 136,022 + GFF3931 0.13 -0.0 136,181 + GFF3931 0.32 -0.2 136,181 + GFF3931 0.32 -0.0 136,181 + GFF3931 0.32 -0.0 136,181 + GFF3931 0.32 -0.0 136,181 + GFF3931 0.32 +1.1 136,181 + GFF3931 0.32 -0.1 136,181 + GFF3931 0.32 -0.6 136,182 - GFF3931 0.32 +0.5 136,182 - GFF3931 0.32 +1.3 136,182 - GFF3931 0.32 +0.2 136,182 - GFF3931 0.32 -0.4 136,182 - GFF3931 0.32 +1.5 136,182 - GFF3931 0.32 +1.0 136,182 - GFF3931 0.32 -0.7 136,184 + GFF3931 0.32 -0.3 136,184 + GFF3931 0.32 +0.2 136,184 + GFF3931 0.32 +0.6 136,184 + GFF3931 0.32 +0.8 136,184 + GFF3931 0.32 -0.5 136,184 + GFF3931 0.32 +0.1 136,184 + GFF3931 0.32 -0.1 136,184 + GFF3931 0.32 -0.5 136,184 + GFF3931 0.32 +1.5 136,184 + GFF3931 0.32 +0.0 136,184 + GFF3931 0.32 -0.0 136,184 + GFF3931 0.32 -0.9 136,184 + GFF3931 0.32 -0.0 136,185 - GFF3931 0.32 -0.5 136,185 - GFF3931 0.32 -0.6 136,185 - GFF3931 0.32 +0.9 136,185 - GFF3931 0.32 -0.3 136,185 - GFF3931 0.32 -0.9 136,185 - GFF3931 0.32 +0.5 136,185 - GFF3931 0.32 -0.1 136,185 - GFF3931 0.32 +1.5 136,185 - GFF3931 0.32 +0.3 136,185 - GFF3931 0.32 -0.0 136,185 - GFF3931 0.32 +1.3 136,185 - GFF3931 0.32 +1.5 136,185 - GFF3931 0.32 -0.8 136,185 - GFF3931 0.32 -0.3 136,185 - GFF3931 0.32 -0.7 136,185 - GFF3931 0.32 +1.1 136,185 - GFF3931 0.32 -0.3 136,202 + GFF3931 0.34 -0.5 136,202 + GFF3931 0.34 +0.0 136,202 + GFF3931 0.34 +0.5 136,202 + GFF3931 0.34 -0.7 136,202 + GFF3931 0.34 -0.3 136,203 - GFF3931 0.34 -0.9 136,203 - GFF3931 0.34 -0.1 136,418 + GFF3931 0.60 -2.1 136,418 + GFF3931 0.60 +0.6 136,418 + GFF3931 0.60 -0.5 136,418 + GFF3931 0.60 -0.9 136,419 - GFF3931 0.60 -0.1 136,419 - GFF3931 0.60 +0.5 136,419 - GFF3931 0.60 -0.5 136,469 + GFF3931 0.65 +1.2 136,469 + GFF3931 0.65 +0.1 136,469 + GFF3931 0.65 +0.7 136,470 - GFF3931 0.66 -0.8 136,470 - GFF3931 0.66 -0.6 136,470 - GFF3931 0.66 +0.5 136,470 - GFF3931 0.66 -1.3 136,523 - GFF3931 0.72 -0.2 136,682 + +0.9 136,682 + +0.1 136,682 + +0.1 136,683 - +0.2 136,683 - -0.2 136,683 - -0.1 136,683 - +0.0
Or see this region's nucleotide sequence