Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF390 and GFF391 are separated by 11 nucleotides GFF391 and GFF392 are separated by 96 nucleotides GFF392 and GFF393 are separated by 81 nucleotides
GFF390 - Ribosome recycling factor, at 422,467 to 423,027
GFF390
GFF391 - Uridylate kinase (EC 2.7.4.22), at 423,039 to 423,761
GFF391
GFF392 - Translation elongation factor Ts, at 423,858 to 424,781
GFF392
GFF393 - SSU ribosomal protein S2p (SAe), at 424,863 to 425,618
GFF393
Position (kb)
423
424
425 Strain fitness (log2 ratio)
-2
-1
0
1 at 423.034 kb on + strand at 423.035 kb on - strand at 423.035 kb on - strand at 423.035 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.794 kb on + strand at 423.794 kb on + strand at 423.802 kb on - strand at 423.802 kb on - strand at 423.802 kb on - strand at 425.766 kb on + strand at 425.766 kb on + strand at 425.766 kb on + strand at 425.766 kb on + strand at 425.766 kb on + strand at 425.766 kb on + strand at 425.767 kb on - strand at 425.767 kb on - strand at 425.767 kb on - strand at 425.767 kb on - strand at 425.767 kb on - strand at 425.767 kb on - strand at 425.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 423,034 + +0.9 423,035 - -0.2 423,035 - -0.2 423,035 - -0.7 423,041 - -0.7 423,041 - -0.0 423,041 - +0.1 423,041 - +1.2 423,041 - -0.2 423,041 - -0.7 423,041 - -0.2 423,041 - +0.5 423,041 - +0.2 423,041 - -0.1 423,794 + -0.3 423,794 + -0.8 423,802 - -0.1 423,802 - -2.1 423,802 - -0.6 425,766 + -0.3 425,766 + -0.4 425,766 + +0.8 425,766 + -1.1 425,766 + -0.5 425,766 + +0.3 425,767 - -0.7 425,767 - -0.8 425,767 - +0.5 425,767 - +0.4 425,767 - -1.0 425,767 - +0.0 425,767 - -1.8
Or see this region's nucleotide sequence