Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3897 and GFF3898 are separated by 7 nucleotides GFF3898 and GFF3899 are separated by 13 nucleotides GFF3899 and GFF3900 are separated by 226 nucleotides
GFF3897 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.5.1), at 98,982 to 100,790
GFF3897
GFF3898 - Succinate dehydrogenase hydrophobic membrane anchor protein, at 100,798 to 101,163
GFF3898
GFF3899 - Succinate dehydrogenase cytochrome b-556 subunit, at 101,177 to 101,623
GFF3899
GFF3900 - Transcriptional regulator, GntR family, at 101,850 to 102,608
GFF3900
Position (kb)
100
101
102 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 101.711 kb on + strand at 101.712 kb on - strand at 101.772 kb on + strand at 101.773 kb on + strand at 101.773 kb on + strand at 101.774 kb on - strand at 101.784 kb on + strand at 101.784 kb on + strand at 101.785 kb on - strand at 101.785 kb on - strand at 101.785 kb on - strand at 101.869 kb on + strand at 101.870 kb on - strand at 101.890 kb on + strand at 101.890 kb on + strand at 101.926 kb on + strand, within GFF3900 at 101.926 kb on + strand, within GFF3900 at 101.926 kb on + strand, within GFF3900 at 101.926 kb on + strand, within GFF3900 at 101.926 kb on + strand, within GFF3900 at 101.926 kb on + strand, within GFF3900 at 101.926 kb on + strand, within GFF3900 at 101.926 kb on + strand, within GFF3900 at 101.927 kb on - strand, within GFF3900 at 101.927 kb on - strand, within GFF3900 at 101.927 kb on - strand, within GFF3900 at 101.927 kb on - strand, within GFF3900 at 101.927 kb on - strand, within GFF3900 at 101.927 kb on - strand, within GFF3900 at 102.073 kb on + strand, within GFF3900 at 102.073 kb on + strand, within GFF3900 at 102.073 kb on + strand, within GFF3900 at 102.073 kb on + strand, within GFF3900 at 102.073 kb on + strand, within GFF3900 at 102.073 kb on + strand, within GFF3900 at 102.073 kb on + strand, within GFF3900 at 102.073 kb on + strand, within GFF3900
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 101,711 + +0.9 101,712 - -0.7 101,772 + -0.0 101,773 + +0.4 101,773 + +0.1 101,774 - -1.0 101,784 + -0.9 101,784 + -0.0 101,785 - +0.3 101,785 - -1.0 101,785 - -0.4 101,869 + +0.4 101,870 - +0.9 101,890 + +0.4 101,890 + +0.3 101,926 + GFF3900 0.10 -0.3 101,926 + GFF3900 0.10 -1.7 101,926 + GFF3900 0.10 +0.5 101,926 + GFF3900 0.10 -0.7 101,926 + GFF3900 0.10 -1.5 101,926 + GFF3900 0.10 +1.1 101,926 + GFF3900 0.10 -0.3 101,926 + GFF3900 0.10 -0.1 101,927 - GFF3900 0.10 -0.8 101,927 - GFF3900 0.10 -1.9 101,927 - GFF3900 0.10 +0.7 101,927 - GFF3900 0.10 -1.3 101,927 - GFF3900 0.10 +0.1 101,927 - GFF3900 0.10 +0.3 102,073 + GFF3900 0.29 +0.2 102,073 + GFF3900 0.29 +2.0 102,073 + GFF3900 0.29 +0.5 102,073 + GFF3900 0.29 -0.0 102,073 + GFF3900 0.29 -1.6 102,073 + GFF3900 0.29 -0.6 102,073 + GFF3900 0.29 -2.7 102,073 + GFF3900 0.29 +0.1
Or see this region's nucleotide sequence