Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3895 and GFF3896 are separated by 29 nucleotides GFF3896 and GFF3897 are separated by 16 nucleotides GFF3897 and GFF3898 are separated by 7 nucleotides GFF3898 and GFF3899 are separated by 13 nucleotides
GFF3895 - Succinate dehydrogenase flavin-adding protein, antitoxin of CptAB toxin-antitoxin, at 97,920 to 98,234
GFF3895
GFF3896 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.5.1), at 98,264 to 98,965
GFF3896
GFF3897 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.5.1), at 98,982 to 100,790
GFF3897
GFF3898 - Succinate dehydrogenase hydrophobic membrane anchor protein, at 100,798 to 101,163
GFF3898
GFF3899 - Succinate dehydrogenase cytochrome b-556 subunit, at 101,177 to 101,623
GFF3899
Position (kb)
98
99
100
101 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 98.247 kb on - strand at 98.265 kb on + strand at 98.266 kb on - strand at 98.266 kb on - strand at 98.266 kb on - strand at 98.266 kb on - strand at 98.266 kb on - strand at 98.266 kb on - strand at 98.969 kb on - strand at 98.969 kb on - strand at 98.969 kb on - strand at 98.969 kb on - strand at 98.969 kb on - strand at 98.984 kb on - strand at 101.711 kb on + strand at 101.712 kb on - strand at 101.772 kb on + strand at 101.773 kb on + strand at 101.773 kb on + strand at 101.774 kb on - strand at 101.784 kb on + strand at 101.784 kb on + strand at 101.785 kb on - strand at 101.785 kb on - strand at 101.785 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 98,247 - +0.4 98,265 + -0.8 98,266 - -1.9 98,266 - -0.6 98,266 - -0.1 98,266 - +1.3 98,266 - +0.4 98,266 - +0.8 98,969 - -0.3 98,969 - +0.3 98,969 - +0.5 98,969 - +2.1 98,969 - -0.3 98,984 - +0.4 101,711 + +0.9 101,712 - -0.7 101,772 + -0.0 101,773 + +0.4 101,773 + +0.1 101,774 - -1.0 101,784 + -0.9 101,784 + -0.0 101,785 - +0.3 101,785 - -1.0 101,785 - -0.4
Or see this region's nucleotide sequence