Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF387 and GFF388 overlap by 4 nucleotides GFF388 and GFF389 are separated by 19 nucleotides GFF389 and GFF390 are separated by 8 nucleotides GFF390 and GFF391 are separated by 11 nucleotides
GFF387 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase (EC 1.1.1.267), at 419,681 to 420,868
GFF387
GFF388 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 420,865 to 421,710
GFF388
GFF389 - Undecaprenyl diphosphate synthase (EC 2.5.1.31), at 421,730 to 422,458
GFF389
GFF390 - Ribosome recycling factor, at 422,467 to 423,027
GFF390
GFF391 - Uridylate kinase (EC 2.7.4.22), at 423,039 to 423,761
GFF391
Position (kb) 421
422
423 Strain fitness (log2 ratio) -1
0
1 at 423.034 kb on + strand at 423.035 kb on - strand at 423.035 kb on - strand at 423.035 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand at 423.041 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 423,034 + +0.9 423,035 - -0.2 423,035 - -0.2 423,035 - -0.7 423,041 - -0.7 423,041 - -0.0 423,041 - +0.1 423,041 - +1.2 423,041 - -0.2 423,041 - -0.7 423,041 - -0.2 423,041 - +0.5 423,041 - +0.2 423,041 - -0.1
Or see this region's nucleotide sequence