Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3883 and GFF3884 are separated by 48 nucleotides GFF3884 and GFF3885 overlap by 4 nucleotides GFF3885 and GFF3886 are separated by 30 nucleotides
GFF3883 - Tryptophan synthase beta chain (EC 4.2.1.20), at 79,895 to 81,181
GFF3883
GFF3884 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 81,230 to 81,937
GFF3884
GFF3885 - tRNA pseudouridine(38-40) synthase (EC 5.4.99.12), at 81,934 to 82,746
GFF3885
GFF3886 - Putative transmembrane protein, at 82,777 to 85,605
GFF3886
Position (kb)
81
82 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 82.007 kb on + strand at 82.008 kb on - strand at 82.025 kb on + strand, within GFF3885 at 82.025 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.422 kb on - strand, within GFF3885 at 82.422 kb on - strand, within GFF3885 at 82.706 kb on + strand at 82.706 kb on + strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.721 kb on + strand at 82.722 kb on - strand at 82.722 kb on - strand at 82.732 kb on + strand at 82.733 kb on - strand at 82.733 kb on - strand at 82.733 kb on - strand at 82.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 81,950 + -0.5 81,950 + +1.0 81,950 + -0.2 81,950 + -0.8 81,950 + +1.0 81,950 + -0.3 81,950 + +0.7 81,950 + +0.5 81,950 + -0.6 81,950 + +0.0 81,951 - -0.5 81,951 - +1.2 81,951 - -0.4 81,951 - +0.1 81,951 - +0.4 81,951 - -0.3 81,951 - -0.1 81,951 - +0.6 81,951 - +0.3 81,951 - +0.6 81,951 - -0.1 81,951 - -0.4 81,951 - +1.5 81,951 - +0.3 81,951 - +2.5 81,951 - +0.8 81,951 - -0.1 82,007 + -0.1 82,008 - +0.1 82,025 + GFF3885 0.11 -2.4 82,025 + GFF3885 0.11 -0.3 82,415 + GFF3885 0.59 +0.5 82,415 + GFF3885 0.59 -0.2 82,415 + GFF3885 0.59 +0.5 82,415 + GFF3885 0.59 -2.4 82,415 + GFF3885 0.59 -0.2 82,415 + GFF3885 0.59 -0.5 82,416 - GFF3885 0.59 -1.0 82,416 - GFF3885 0.59 -0.8 82,416 - GFF3885 0.59 -0.1 82,416 - GFF3885 0.59 -0.3 82,416 - GFF3885 0.59 -0.5 82,416 - GFF3885 0.59 -0.1 82,416 - GFF3885 0.59 -0.3 82,416 - GFF3885 0.59 -0.6 82,416 - GFF3885 0.59 -0.4 82,416 - GFF3885 0.59 -1.1 82,416 - GFF3885 0.59 +0.5 82,421 + GFF3885 0.60 -0.2 82,421 + GFF3885 0.60 -0.2 82,421 + GFF3885 0.60 -0.5 82,422 - GFF3885 0.60 -0.9 82,422 - GFF3885 0.60 -0.4 82,706 + -0.8 82,706 + +0.4 82,707 - +0.3 82,707 - -1.3 82,707 - -0.3 82,707 - -0.0 82,707 - -0.2 82,721 + +1.1 82,722 - -0.3 82,722 - +0.3 82,732 + -0.0 82,733 - +0.9 82,733 - +0.2 82,733 - -0.1 82,733 - -0.3
Or see this region's nucleotide sequence