Strain Fitness in Variovorax sp. SCN45 around GFF3882

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3880 and GFF3881 are separated by 69 nucleotidesGFF3881 and GFF3882 are separated by 25 nucleotidesGFF3882 and GFF3883 overlap by 1 nucleotides GFF3880 - Cell division integral membrane protein, YggT and half-length relatives, at 77,522 to 78,082 GFF3880 GFF3881 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 78,152 to 79,024 GFF3881 GFF3882 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 79,050 to 79,895 GFF3882 GFF3883 - Tryptophan synthase beta chain (EC 4.2.1.20), at 79,895 to 81,181 GFF3883 Position (kb) 79 80Strain fitness (log2 ratio) -2 -1 0 1at 78.060 kb on + strandat 78.061 kb on - strandat 78.065 kb on + strandat 78.065 kb on + strandat 78.065 kb on + strandat 78.065 kb on + strandat 78.065 kb on + strandat 78.066 kb on - strandat 78.066 kb on - strandat 78.066 kb on - strandat 78.066 kb on - strandat 78.066 kb on - strandat 78.066 kb on - strandat 78.066 kb on - strandat 79.357 kb on + strand, within GFF3882at 79.941 kb on + strandat 79.941 kb on + strandat 79.942 kb on - strandat 79.942 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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78,060 + -0.5
78,061 - -1.3
78,065 + -0.3
78,065 + +0.0
78,065 + -0.1
78,065 + -0.7
78,065 + -0.4
78,066 - +1.0
78,066 - -0.4
78,066 - -0.8
78,066 - -0.2
78,066 - +0.2
78,066 - +0.4
78,066 - +1.0
79,357 + GFF3882 0.36 -1.9
79,941 + -0.7
79,941 + +1.3
79,942 - -0.5
79,942 - +0.3

Or see this region's nucleotide sequence