Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3869 and GFF3870 are separated by 17 nucleotides GFF3870 and GFF3871 are separated by 7 nucleotides GFF3871 and GFF3872 are separated by 136 nucleotides GFF3872 and GFF3873 overlap by 4 nucleotides
GFF3869 - Putative transmembrane protein, at 68,124 to 68,492
GFF3869
GFF3870 - Ribonuclease III (EC 3.1.26.3), at 68,510 to 69,196
GFF3870
GFF3871 - GTP-binding protein Era, at 69,204 to 70,169
GFF3871
GFF3872 - DNA recombination and repair protein RecO, at 70,306 to 71,076
GFF3872
GFF3873 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 71,073 to 71,834
GFF3873
Position (kb) 69
70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 68.418 kb on + strand, within GFF3869 at 68.418 kb on + strand, within GFF3869 at 68.418 kb on + strand, within GFF3869 at 68.418 kb on + strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.419 kb on - strand, within GFF3869 at 68.424 kb on + strand, within GFF3869 at 68.424 kb on + strand, within GFF3869 at 68.424 kb on + strand, within GFF3869 at 68.424 kb on + strand, within GFF3869 at 68.424 kb on + strand, within GFF3869 at 68.424 kb on + strand, within GFF3869 at 68.425 kb on - strand, within GFF3869 at 68.425 kb on - strand, within GFF3869 at 68.425 kb on - strand, within GFF3869 at 68.457 kb on + strand at 68.458 kb on - strand at 68.458 kb on - strand at 68.458 kb on - strand at 68.458 kb on - strand at 68.472 kb on + strand at 68.472 kb on + strand at 68.472 kb on + strand at 68.472 kb on + strand at 68.472 kb on + strand at 68.472 kb on + strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.473 kb on - strand at 68.504 kb on - strand at 68.504 kb on - strand at 68.674 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.675 kb on + strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.676 kb on - strand, within GFF3870 at 68.677 kb on + strand, within GFF3870 at 68.677 kb on + strand, within GFF3870 at 68.876 kb on + strand, within GFF3870 at 68.877 kb on - strand, within GFF3870 at 68.924 kb on + strand, within GFF3870 at 68.925 kb on - strand, within GFF3870 at 68.925 kb on - strand, within GFF3870 at 69.026 kb on + strand, within GFF3870 at 69.027 kb on - strand, within GFF3870 at 70.162 kb on - strand at 70.167 kb on + strand at 70.168 kb on - strand at 70.168 kb on - strand at 70.294 kb on + strand at 70.294 kb on + strand at 70.295 kb on - strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.339 kb on + strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.354 kb on + strand at 70.355 kb on - strand at 70.495 kb on + strand, within GFF3872 at 70.495 kb on + strand, within GFF3872 at 70.495 kb on + strand, within GFF3872 at 70.495 kb on + strand, within GFF3872 at 70.495 kb on + strand, within GFF3872 at 70.496 kb on - strand, within GFF3872 at 70.496 kb on - strand, within GFF3872 at 70.496 kb on - strand, within GFF3872 at 70.496 kb on - strand, within GFF3872 at 70.585 kb on + strand, within GFF3872 at 70.585 kb on + strand, within GFF3872 at 70.585 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.588 kb on + strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.589 kb on - strand, within GFF3872 at 70.654 kb on + strand, within GFF3872 at 70.654 kb on + strand, within GFF3872 at 70.654 kb on + strand, within GFF3872 at 70.655 kb on - strand, within GFF3872 at 70.655 kb on - strand, within GFF3872 at 70.822 kb on + strand, within GFF3872 at 70.823 kb on - strand, within GFF3872 at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.040 kb on + strand at 71.040 kb on + strand at 71.041 kb on - strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 68,418 + GFF3869 0.80 -0.8 68,418 + GFF3869 0.80 -0.6 68,418 + GFF3869 0.80 -1.5 68,418 + GFF3869 0.80 -0.4 68,419 - GFF3869 0.80 +0.4 68,419 - GFF3869 0.80 +0.5 68,419 - GFF3869 0.80 +0.8 68,419 - GFF3869 0.80 -0.0 68,419 - GFF3869 0.80 -0.7 68,419 - GFF3869 0.80 -1.6 68,419 - GFF3869 0.80 +0.4 68,419 - GFF3869 0.80 -0.5 68,424 + GFF3869 0.81 +1.0 68,424 + GFF3869 0.81 +0.8 68,424 + GFF3869 0.81 +0.0 68,424 + GFF3869 0.81 -0.2 68,424 + GFF3869 0.81 +0.9 68,424 + GFF3869 0.81 -0.1 68,425 - GFF3869 0.82 +0.1 68,425 - GFF3869 0.82 -0.0 68,425 - GFF3869 0.82 +0.1 68,457 + -0.6 68,458 - -0.0 68,458 - -0.4 68,458 - -1.0 68,458 - -0.3 68,472 + +0.3 68,472 + -0.7 68,472 + +0.2 68,472 + -0.4 68,472 + +0.2 68,472 + -1.0 68,473 - -0.6 68,473 - -1.5 68,473 - -0.3 68,473 - -0.4 68,473 - -0.4 68,473 - -0.2 68,473 - -0.9 68,473 - +0.3 68,473 - +0.2 68,473 - -0.0 68,473 - -0.2 68,473 - +0.3 68,504 - +0.5 68,504 - -1.3 68,674 + GFF3870 0.24 +1.2 68,675 + GFF3870 0.24 -0.6 68,675 + GFF3870 0.24 +0.5 68,675 + GFF3870 0.24 -0.5 68,675 + GFF3870 0.24 -0.5 68,675 + GFF3870 0.24 -1.0 68,675 + GFF3870 0.24 -1.9 68,675 + GFF3870 0.24 -0.3 68,675 + GFF3870 0.24 -0.3 68,675 + GFF3870 0.24 -0.8 68,675 + GFF3870 0.24 -0.7 68,676 - GFF3870 0.24 -0.5 68,676 - GFF3870 0.24 -0.8 68,676 - GFF3870 0.24 -0.3 68,676 - GFF3870 0.24 +0.1 68,676 - GFF3870 0.24 +0.8 68,676 - GFF3870 0.24 -1.3 68,676 - GFF3870 0.24 +0.4 68,676 - GFF3870 0.24 +0.7 68,676 - GFF3870 0.24 -1.1 68,676 - GFF3870 0.24 -0.9 68,676 - GFF3870 0.24 -1.0 68,676 - GFF3870 0.24 +0.5 68,677 + GFF3870 0.24 -0.2 68,677 + GFF3870 0.24 -0.8 68,876 + GFF3870 0.53 -1.9 68,877 - GFF3870 0.53 -0.6 68,924 + GFF3870 0.60 -0.3 68,925 - GFF3870 0.60 +0.5 68,925 - GFF3870 0.60 -1.2 69,026 + GFF3870 0.75 -1.0 69,027 - GFF3870 0.75 +0.9 70,162 - -0.4 70,167 + -1.3 70,168 - +1.9 70,168 - +0.5 70,294 + +0.2 70,294 + -0.5 70,295 - -0.8 70,339 + +0.2 70,339 + -0.1 70,339 + +0.4 70,339 + -0.7 70,339 + -0.4 70,339 + -0.6 70,339 + -1.5 70,339 + -0.7 70,339 + +0.7 70,339 + +0.2 70,339 + +0.5 70,339 + -0.4 70,339 + -1.8 70,339 + +1.3 70,339 + +0.5 70,339 + +1.1 70,339 + -0.2 70,339 + -0.2 70,340 - -0.9 70,340 - -1.9 70,340 - +0.3 70,340 - +0.1 70,340 - -1.0 70,340 - -3.1 70,340 - -0.1 70,340 - -3.2 70,340 - -0.3 70,340 - +1.2 70,354 + -0.3 70,355 - -0.1 70,495 + GFF3872 0.25 +1.5 70,495 + GFF3872 0.25 +0.6 70,495 + GFF3872 0.25 +0.4 70,495 + GFF3872 0.25 -0.8 70,495 + GFF3872 0.25 -0.3 70,496 - GFF3872 0.25 -1.6 70,496 - GFF3872 0.25 -1.2 70,496 - GFF3872 0.25 +0.5 70,496 - GFF3872 0.25 -0.0 70,585 + GFF3872 0.36 -0.6 70,585 + GFF3872 0.36 +0.0 70,585 + GFF3872 0.36 +1.5 70,588 + GFF3872 0.37 -0.3 70,588 + GFF3872 0.37 +1.7 70,588 + GFF3872 0.37 -0.3 70,588 + GFF3872 0.37 -0.3 70,588 + GFF3872 0.37 -0.2 70,588 + GFF3872 0.37 -0.0 70,588 + GFF3872 0.37 +0.5 70,588 + GFF3872 0.37 -0.3 70,588 + GFF3872 0.37 +0.0 70,588 + GFF3872 0.37 +1.5 70,588 + GFF3872 0.37 -0.6 70,588 + GFF3872 0.37 -0.6 70,588 + GFF3872 0.37 -0.4 70,588 + GFF3872 0.37 +0.1 70,588 + GFF3872 0.37 -0.3 70,588 + GFF3872 0.37 -0.8 70,588 + GFF3872 0.37 -0.4 70,588 + GFF3872 0.37 +1.2 70,588 + GFF3872 0.37 +0.1 70,589 - GFF3872 0.37 +0.1 70,589 - GFF3872 0.37 -0.2 70,589 - GFF3872 0.37 +0.4 70,589 - GFF3872 0.37 +0.3 70,589 - GFF3872 0.37 +0.7 70,589 - GFF3872 0.37 +0.2 70,589 - GFF3872 0.37 -0.3 70,589 - GFF3872 0.37 -0.3 70,589 - GFF3872 0.37 -1.0 70,654 + GFF3872 0.45 +0.4 70,654 + GFF3872 0.45 -0.5 70,654 + GFF3872 0.45 +1.8 70,655 - GFF3872 0.45 -1.9 70,655 - GFF3872 0.45 -1.2 70,822 + GFF3872 0.67 -0.5 70,823 - GFF3872 0.67 +0.5 71,014 + -0.3 71,014 + -1.9 71,014 + -0.8 71,014 + -1.2 71,014 + -0.3 71,014 + +0.1 71,014 + -0.0 71,015 - +0.0 71,015 - -0.4 71,015 - -0.2 71,015 - -0.5 71,015 - +1.2 71,015 - -0.4 71,015 - -0.9 71,015 - -1.3 71,015 - +0.1 71,040 + -0.3 71,040 + -0.6 71,041 - -0.6 71,072 + +0.7 71,072 + -0.8 71,072 + -0.8 71,072 + -0.6 71,072 + +1.3 71,072 + +0.5 71,072 + -0.2 71,072 + -0.5 71,073 - +0.9 71,073 - -0.0 71,073 - -0.6 71,073 - +0.6 71,073 - -1.0 71,073 - -0.2
Or see this region's nucleotide sequence