Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3774 and GFF3775 overlap by 4 nucleotides GFF3775 and GFF3776 are separated by 167 nucleotides GFF3776 and GFF3777 are separated by 6 nucleotides
GFF3774 - hypothetical protein, at 265,400 to 266,797
GFF3774
GFF3775 - Two-component transcriptional response regulator, OmpR family, at 266,794 to 267,468
GFF3775
GFF3776 - no description, at 267,636 to 268,025
GFF3776
GFF3777 - Prolyl endopeptidase (EC 3.4.21.26), at 268,032 to 270,083
GFF3777
Position (kb) 266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 265.797 kb on + strand, within GFF3774 at 266.354 kb on + strand, within GFF3774 at 266.355 kb on - strand, within GFF3774 at 266.355 kb on - strand, within GFF3774 at 266.711 kb on + strand at 267.121 kb on + strand, within GFF3775 at 267.121 kb on + strand, within GFF3775 at 267.122 kb on - strand, within GFF3775 at 267.122 kb on - strand, within GFF3775 at 267.176 kb on + strand, within GFF3775 at 267.177 kb on - strand, within GFF3775 at 267.252 kb on + strand, within GFF3775 at 267.253 kb on - strand, within GFF3775 at 267.531 kb on + strand at 267.554 kb on - strand at 267.631 kb on + strand at 267.631 kb on + strand at 267.631 kb on + strand at 267.632 kb on - strand at 267.632 kb on - strand at 267.632 kb on - strand at 267.780 kb on + strand, within GFF3776 at 267.786 kb on + strand, within GFF3776 at 267.786 kb on + strand, within GFF3776 at 267.786 kb on + strand, within GFF3776 at 267.787 kb on - strand, within GFF3776 at 267.787 kb on - strand, within GFF3776 at 267.787 kb on - strand, within GFF3776 at 267.912 kb on + strand, within GFF3776 at 268.055 kb on - strand at 268.055 kb on - strand at 268.055 kb on - strand at 268.055 kb on - strand at 268.055 kb on - strand at 268.055 kb on - strand at 268.060 kb on + strand at 268.060 kb on + strand at 268.061 kb on - strand at 268.061 kb on - strand at 268.061 kb on - strand at 268.132 kb on + strand at 268.267 kb on + strand, within GFF3777 at 268.267 kb on + strand, within GFF3777 at 268.268 kb on - strand, within GFF3777 at 268.268 kb on - strand, within GFF3777 at 268.322 kb on - strand, within GFF3777 at 268.406 kb on - strand, within GFF3777 at 268.417 kb on + strand, within GFF3777 at 268.418 kb on - strand, within GFF3777
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 265,797 + GFF3774 0.28 -1.3 266,354 + GFF3774 0.68 -0.0 266,355 - GFF3774 0.68 -0.7 266,355 - GFF3774 0.68 +0.5 266,711 + -0.1 267,121 + GFF3775 0.48 -0.8 267,121 + GFF3775 0.48 -3.2 267,122 - GFF3775 0.49 +1.4 267,122 - GFF3775 0.49 -1.5 267,176 + GFF3775 0.57 -0.7 267,177 - GFF3775 0.57 -0.4 267,252 + GFF3775 0.68 +0.2 267,253 - GFF3775 0.68 -1.4 267,531 + +0.4 267,554 - +1.4 267,631 + +1.0 267,631 + +1.8 267,631 + +0.6 267,632 - -1.5 267,632 - -0.8 267,632 - +0.4 267,780 + GFF3776 0.37 +0.0 267,786 + GFF3776 0.38 -0.4 267,786 + GFF3776 0.38 -0.1 267,786 + GFF3776 0.38 +0.7 267,787 - GFF3776 0.39 +1.2 267,787 - GFF3776 0.39 -0.3 267,787 - GFF3776 0.39 +0.5 267,912 + GFF3776 0.71 +0.3 268,055 - +1.8 268,055 - +0.5 268,055 - -0.4 268,055 - -1.9 268,055 - -0.2 268,055 - -1.5 268,060 + -0.3 268,060 + +0.3 268,061 - -0.3 268,061 - -0.5 268,061 - +0.1 268,132 + -0.1 268,267 + GFF3777 0.11 -0.3 268,267 + GFF3777 0.11 +0.2 268,268 - GFF3777 0.12 -0.3 268,268 - GFF3777 0.12 -0.6 268,322 - GFF3777 0.14 +0.2 268,406 - GFF3777 0.18 -0.6 268,417 + GFF3777 0.19 +0.0 268,418 - GFF3777 0.19 -0.5
Or see this region's nucleotide sequence