Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF376 and GFF377 overlap by 8 nucleotides GFF377 and GFF378 are separated by 140 nucleotides GFF378 and GFF379 are separated by 7 nucleotides
GFF376 - Carbamoyl-phosphate synthase large chain (EC 6.3.5.5), at 405,737 to 408,985
GFF376
GFF377 - Carbamoyl-phosphate synthase small chain (EC 6.3.5.5), at 408,978 to 410,177
GFF377
GFF378 - RNA (nucleoside 2'-O)-methyltransferase, at 410,318 to 411,103
GFF378
GFF379 - Ribonuclease HII (EC 3.1.26.4), at 411,111 to 411,821
GFF379
Position (kb) 408
409
410
411 Strain fitness (log2 ratio) -2
-1
0
1 at 410.196 kb on - strand at 410.196 kb on - strand at 410.196 kb on - strand at 410.300 kb on + strand at 410.301 kb on - strand at 410.301 kb on - strand at 410.301 kb on - strand at 410.301 kb on - strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.319 kb on + strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.320 kb on - strand at 410.588 kb on + strand, within GFF378 at 410.588 kb on + strand, within GFF378 at 410.588 kb on + strand, within GFF378 at 410.589 kb on - strand, within GFF378 at 410.589 kb on - strand, within GFF378 at 411.175 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 410,196 - +1.6 410,196 - -0.4 410,196 - -1.5 410,300 + +0.8 410,301 - -0.8 410,301 - +0.7 410,301 - +0.6 410,301 - -0.1 410,319 + -0.3 410,319 + -0.4 410,319 + +0.1 410,319 + -0.1 410,319 + +0.3 410,319 + +0.0 410,320 - -1.1 410,320 - +0.2 410,320 - -0.4 410,320 - -0.2 410,320 - +0.0 410,320 - -0.2 410,320 - +1.5 410,320 - -1.8 410,588 + GFF378 0.34 -0.8 410,588 + GFF378 0.34 +0.5 410,588 + GFF378 0.34 -1.0 410,589 - GFF378 0.34 +1.2 410,589 - GFF378 0.34 -0.8 411,175 - -0.4
Or see this region's nucleotide sequence