Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF374 and GFF375 are separated by 66 nucleotides GFF375 and GFF376 are separated by 8 nucleotides GFF376 and GFF377 overlap by 8 nucleotides
GFF374 - Transcription elongation factor GreA, at 404,316 to 404,792
GFF374
GFF375 - hypothetical protein, at 404,859 to 405,728
GFF375
GFF376 - Carbamoyl-phosphate synthase large chain (EC 6.3.5.5), at 405,737 to 408,985
GFF376
GFF377 - Carbamoyl-phosphate synthase small chain (EC 6.3.5.5), at 408,978 to 410,177
GFF377
Position (kb) 405
406
407
408
409 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 404.808 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.920 kb on + strand at 404.921 kb on - strand at 404.921 kb on - strand at 404.921 kb on - strand at 404.921 kb on - strand at 404.921 kb on - strand at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.962 kb on + strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 404.963 kb on - strand, within GFF375 at 405.061 kb on + strand, within GFF375 at 405.061 kb on + strand, within GFF375 at 405.061 kb on + strand, within GFF375 at 405.062 kb on - strand, within GFF375 at 405.062 kb on - strand, within GFF375 at 405.112 kb on + strand, within GFF375 at 405.113 kb on - strand, within GFF375 at 405.175 kb on + strand, within GFF375 at 405.175 kb on + strand, within GFF375 at 405.175 kb on + strand, within GFF375 at 405.175 kb on + strand, within GFF375 at 405.176 kb on - strand, within GFF375 at 405.295 kb on + strand, within GFF375 at 405.295 kb on + strand, within GFF375 at 405.295 kb on + strand, within GFF375 at 405.295 kb on + strand, within GFF375 at 405.295 kb on + strand, within GFF375 at 405.295 kb on + strand, within GFF375 at 405.295 kb on + strand, within GFF375 at 405.296 kb on - strand, within GFF375 at 405.296 kb on - strand, within GFF375 at 405.296 kb on - strand, within GFF375 at 405.296 kb on - strand, within GFF375 at 405.296 kb on - strand, within GFF375 at 405.296 kb on - strand, within GFF375 at 405.385 kb on + strand, within GFF375 at 405.386 kb on - strand, within GFF375 at 405.418 kb on + strand, within GFF375 at 405.418 kb on + strand, within GFF375 at 405.418 kb on + strand, within GFF375 at 405.418 kb on + strand, within GFF375 at 405.418 kb on + strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.419 kb on - strand, within GFF375 at 405.631 kb on + strand, within GFF375 at 405.631 kb on + strand, within GFF375 at 405.631 kb on + strand, within GFF375 at 405.631 kb on + strand, within GFF375 at 405.631 kb on + strand, within GFF375 at 405.631 kb on + strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375 at 405.632 kb on - strand, within GFF375
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 404,808 + -1.8 404,920 + +0.5 404,920 + +1.8 404,920 + -0.6 404,920 + -0.3 404,920 + -0.1 404,920 + +0.5 404,920 + -0.2 404,920 + +0.5 404,920 + +0.8 404,920 + -0.8 404,921 - +0.3 404,921 - -0.5 404,921 - -0.5 404,921 - -1.1 404,921 - -1.2 404,962 + GFF375 0.12 -1.2 404,962 + GFF375 0.12 +0.8 404,962 + GFF375 0.12 +0.7 404,962 + GFF375 0.12 -0.5 404,962 + GFF375 0.12 +0.6 404,962 + GFF375 0.12 +1.5 404,962 + GFF375 0.12 -2.1 404,962 + GFF375 0.12 -1.0 404,962 + GFF375 0.12 +0.1 404,962 + GFF375 0.12 -0.0 404,962 + GFF375 0.12 +0.1 404,962 + GFF375 0.12 +0.8 404,962 + GFF375 0.12 +1.2 404,962 + GFF375 0.12 -0.9 404,962 + GFF375 0.12 +0.1 404,962 + GFF375 0.12 -0.2 404,962 + GFF375 0.12 -0.5 404,962 + GFF375 0.12 -1.5 404,962 + GFF375 0.12 -0.5 404,962 + GFF375 0.12 -1.4 404,962 + GFF375 0.12 -0.2 404,962 + GFF375 0.12 -0.5 404,962 + GFF375 0.12 -1.4 404,962 + GFF375 0.12 +0.0 404,963 - GFF375 0.12 +0.4 404,963 - GFF375 0.12 +0.4 404,963 - GFF375 0.12 -0.4 404,963 - GFF375 0.12 +0.4 404,963 - GFF375 0.12 -0.1 404,963 - GFF375 0.12 -0.6 404,963 - GFF375 0.12 +0.9 404,963 - GFF375 0.12 -1.6 404,963 - GFF375 0.12 -0.2 404,963 - GFF375 0.12 -0.6 404,963 - GFF375 0.12 +0.2 404,963 - GFF375 0.12 -0.1 404,963 - GFF375 0.12 +0.2 404,963 - GFF375 0.12 -0.8 404,963 - GFF375 0.12 -1.9 404,963 - GFF375 0.12 +0.5 404,963 - GFF375 0.12 +0.4 404,963 - GFF375 0.12 -0.7 404,963 - GFF375 0.12 +0.6 404,963 - GFF375 0.12 -1.0 404,963 - GFF375 0.12 +0.1 404,963 - GFF375 0.12 -0.2 404,963 - GFF375 0.12 -0.1 404,963 - GFF375 0.12 -0.3 405,061 + GFF375 0.23 +0.0 405,061 + GFF375 0.23 -0.3 405,061 + GFF375 0.23 +0.1 405,062 - GFF375 0.23 -0.4 405,062 - GFF375 0.23 -2.3 405,112 + GFF375 0.29 -0.8 405,113 - GFF375 0.29 -0.7 405,175 + GFF375 0.36 -0.0 405,175 + GFF375 0.36 -0.4 405,175 + GFF375 0.36 -0.4 405,175 + GFF375 0.36 -0.1 405,176 - GFF375 0.36 +0.2 405,295 + GFF375 0.50 -1.9 405,295 + GFF375 0.50 -0.9 405,295 + GFF375 0.50 +0.2 405,295 + GFF375 0.50 -0.6 405,295 + GFF375 0.50 +1.9 405,295 + GFF375 0.50 -0.5 405,295 + GFF375 0.50 +0.3 405,296 - GFF375 0.50 +0.1 405,296 - GFF375 0.50 -0.1 405,296 - GFF375 0.50 +1.2 405,296 - GFF375 0.50 -0.1 405,296 - GFF375 0.50 -0.5 405,296 - GFF375 0.50 -0.6 405,385 + GFF375 0.60 -0.8 405,386 - GFF375 0.61 -1.8 405,418 + GFF375 0.64 -1.8 405,418 + GFF375 0.64 -1.2 405,418 + GFF375 0.64 +0.1 405,418 + GFF375 0.64 -1.8 405,418 + GFF375 0.64 +1.2 405,419 - GFF375 0.64 +0.1 405,419 - GFF375 0.64 -0.1 405,419 - GFF375 0.64 -0.2 405,419 - GFF375 0.64 -0.5 405,419 - GFF375 0.64 +2.8 405,419 - GFF375 0.64 +0.1 405,419 - GFF375 0.64 +0.1 405,419 - GFF375 0.64 -1.7 405,631 + GFF375 0.89 +0.0 405,631 + GFF375 0.89 +0.2 405,631 + GFF375 0.89 +1.8 405,631 + GFF375 0.89 +0.4 405,631 + GFF375 0.89 -0.5 405,631 + GFF375 0.89 +0.0 405,632 - GFF375 0.89 -0.3 405,632 - GFF375 0.89 -2.0 405,632 - GFF375 0.89 -0.3 405,632 - GFF375 0.89 +1.0 405,632 - GFF375 0.89 +0.3 405,632 - GFF375 0.89 -0.1 405,632 - GFF375 0.89 -1.0 405,632 - GFF375 0.89 -1.8 405,632 - GFF375 0.89 +0.7
Or see this region's nucleotide sequence