Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3698 and GFF3699 are separated by 22 nucleotides GFF3699 and GFF3700 are separated by 59 nucleotides GFF3700 and GFF3701 are separated by 126 nucleotides GFF3701 and GFF3702 are separated by 5 nucleotides
GFF3698 - ATP-dependent, 3'-5' DNA helicase with strand annealing activity, at 198,030 to 200,786
GFF3698
GFF3699 - Thioredoxin 2, at 200,809 to 201,258
GFF3699
GFF3700 - FIG006285: hypothetical protein, at 201,318 to 202,016
GFF3700
GFF3701 - FIG004694: Hypothetical protein, at 202,143 to 202,670
GFF3701
GFF3702 - Beta-propeller domains of methanol dehydrogenase type, at 202,676 to 203,533
GFF3702
Position (kb) 201
202
203 Strain fitness (log2 ratio) -1
0
1 at 200.412 kb on + strand, within GFF3698 at 200.413 kb on - strand, within GFF3698 at 200.413 kb on - strand, within GFF3698 at 201.483 kb on + strand, within GFF3700 at 202.152 kb on + strand at 202.152 kb on + strand at 202.152 kb on + strand at 202.153 kb on - strand at 202.153 kb on - strand at 202.153 kb on - strand at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.384 kb on + strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.385 kb on - strand, within GFF3701 at 202.480 kb on + strand, within GFF3701 at 202.480 kb on + strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 202.481 kb on - strand, within GFF3701 at 202.481 kb on - strand, within GFF3701
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 200,412 + GFF3698 0.86 -0.2 200,413 - GFF3698 0.86 +0.8 200,413 - GFF3698 0.86 +0.0 201,483 + GFF3700 0.24 -1.3 202,152 + -0.2 202,152 + +0.3 202,152 + -0.3 202,153 - -0.8 202,153 - -0.2 202,153 - +0.5 202,384 + GFF3701 0.46 -0.2 202,384 + GFF3701 0.46 -0.1 202,384 + GFF3701 0.46 -0.2 202,384 + GFF3701 0.46 -0.8 202,384 + GFF3701 0.46 -0.7 202,385 - GFF3701 0.46 +0.0 202,385 - GFF3701 0.46 +0.3 202,385 - GFF3701 0.46 -0.2 202,385 - GFF3701 0.46 -0.8 202,480 + GFF3701 0.64 -0.4 202,480 + GFF3701 0.64 -0.1 202,481 - GFF3701 0.64 +1.0 202,481 - GFF3701 0.64 -0.4 202,481 - GFF3701 0.64 +0.4
Or see this region's nucleotide sequence