Strain Fitness in Variovorax sp. SCN45 around GFF3699

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3698 and GFF3699 are separated by 22 nucleotidesGFF3699 and GFF3700 are separated by 59 nucleotidesGFF3700 and GFF3701 are separated by 126 nucleotides GFF3698 - ATP-dependent, 3'-5' DNA helicase with strand annealing activity, at 198,030 to 200,786 GFF3698 GFF3699 - Thioredoxin 2, at 200,809 to 201,258 GFF3699 GFF3700 - FIG006285: hypothetical protein, at 201,318 to 202,016 GFF3700 GFF3701 - FIG004694: Hypothetical protein, at 202,143 to 202,670 GFF3701 Position (kb) 200 201 202Strain fitness (log2 ratio) -1 0 1at 199.905 kb on + strand, within GFF3698at 199.905 kb on + strand, within GFF3698at 199.906 kb on - strand, within GFF3698at 199.942 kb on + strand, within GFF3698at 199.942 kb on + strand, within GFF3698at 199.942 kb on + strand, within GFF3698at 199.942 kb on + strand, within GFF3698at 200.136 kb on + strand, within GFF3698at 200.412 kb on + strand, within GFF3698at 200.413 kb on - strand, within GFF3698at 200.413 kb on - strand, within GFF3698at 201.483 kb on + strand, within GFF3700at 202.152 kb on + strandat 202.152 kb on + strandat 202.152 kb on + strandat 202.153 kb on - strandat 202.153 kb on - strandat 202.153 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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199,905 + GFF3698 0.68 -0.7
199,905 + GFF3698 0.68 +0.4
199,906 - GFF3698 0.68 -0.1
199,942 + GFF3698 0.69 -0.8
199,942 + GFF3698 0.69 -0.8
199,942 + GFF3698 0.69 +0.2
199,942 + GFF3698 0.69 -0.1
200,136 + GFF3698 0.76 +1.6
200,412 + GFF3698 0.86 -0.2
200,413 - GFF3698 0.86 +0.8
200,413 - GFF3698 0.86 +0.0
201,483 + GFF3700 0.24 -1.3
202,152 + -0.2
202,152 + +0.3
202,152 + -0.3
202,153 - -0.8
202,153 - -0.2
202,153 - +0.5

Or see this region's nucleotide sequence