Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF367 and GFF368 overlap by 4 nucleotides GFF368 and GFF369 are separated by 109 nucleotides GFF369 and GFF370 are separated by 167 nucleotides GFF370 and GFF371 are separated by 15 nucleotides
GFF367 - Phosphoglucosamine mutase (EC 5.4.2.10), at 397,555 to 398,892
GFF367
GFF368 - Dihydropteroate synthase (EC 2.5.1.15), at 398,889 to 399,707
GFF368
GFF369 - Cell division-associated, ATP-dependent zinc metalloprotease FtsH, at 399,817 to 401,733
GFF369
GFF370 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase (EC 2.1.1.166), at 401,901 to 402,662
GFF370
GFF371 - RNA-binding protein YhbY, at 402,678 to 403,082
GFF371
Position (kb) 399
400
401
402 Strain fitness (log2 ratio) -2
-1
0
1
2 at 401.775 kb on - strand at 401.775 kb on - strand at 401.775 kb on - strand at 401.854 kb on - strand at 401.854 kb on - strand at 401.879 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.900 kb on - strand at 401.910 kb on - strand at 401.910 kb on - strand at 401.910 kb on - strand at 401.923 kb on - strand at 401.923 kb on - strand at 401.923 kb on - strand at 401.923 kb on - strand at 401.923 kb on - strand at 402.364 kb on + strand, within GFF370 at 402.364 kb on + strand, within GFF370 at 402.365 kb on - strand, within GFF370 at 402.457 kb on + strand, within GFF370 at 402.458 kb on - strand, within GFF370 at 402.458 kb on - strand, within GFF370 at 402.458 kb on - strand, within GFF370 at 402.458 kb on - strand, within GFF370 at 402.502 kb on + strand, within GFF370 at 402.502 kb on + strand, within GFF370 at 402.502 kb on + strand, within GFF370 at 402.502 kb on + strand, within GFF370 at 402.502 kb on + strand, within GFF370 at 402.502 kb on + strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.503 kb on - strand, within GFF370 at 402.515 kb on + strand, within GFF370 at 402.515 kb on + strand, within GFF370 at 402.515 kb on + strand, within GFF370 at 402.515 kb on + strand, within GFF370 at 402.516 kb on - strand, within GFF370 at 402.516 kb on - strand, within GFF370 at 402.583 kb on + strand, within GFF370 at 402.583 kb on + strand, within GFF370 at 402.584 kb on - strand, within GFF370 at 402.593 kb on + strand at 402.593 kb on + strand at 402.594 kb on - strand at 402.620 kb on + strand at 402.620 kb on + strand at 402.621 kb on - strand at 402.698 kb on + strand at 402.698 kb on + strand at 402.699 kb on - strand at 402.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 401,775 - +0.2 401,775 - +0.2 401,775 - +0.2 401,854 - -0.5 401,854 - +0.1 401,879 - +0.6 401,900 - +1.2 401,900 - -0.0 401,900 - -0.1 401,900 - +1.6 401,900 - +1.1 401,900 - -0.4 401,900 - -0.5 401,900 - +0.9 401,900 - -0.7 401,900 - +0.4 401,900 - +1.7 401,900 - +0.3 401,910 - -0.5 401,910 - +0.7 401,910 - -1.4 401,923 - -1.0 401,923 - -0.7 401,923 - -0.1 401,923 - -0.8 401,923 - -0.4 402,364 + GFF370 0.61 -0.5 402,364 + GFF370 0.61 +0.8 402,365 - GFF370 0.61 +0.4 402,457 + GFF370 0.73 -0.3 402,458 - GFF370 0.73 -0.5 402,458 - GFF370 0.73 -0.9 402,458 - GFF370 0.73 -0.3 402,458 - GFF370 0.73 -0.1 402,502 + GFF370 0.79 -0.3 402,502 + GFF370 0.79 +1.2 402,502 + GFF370 0.79 -0.2 402,502 + GFF370 0.79 +0.6 402,502 + GFF370 0.79 -0.3 402,502 + GFF370 0.79 +1.2 402,503 - GFF370 0.79 -0.7 402,503 - GFF370 0.79 -0.2 402,503 - GFF370 0.79 -1.3 402,503 - GFF370 0.79 +0.2 402,503 - GFF370 0.79 -0.6 402,503 - GFF370 0.79 -0.2 402,503 - GFF370 0.79 -0.5 402,503 - GFF370 0.79 -0.2 402,503 - GFF370 0.79 +0.2 402,503 - GFF370 0.79 -0.5 402,503 - GFF370 0.79 +0.4 402,503 - GFF370 0.79 -0.9 402,515 + GFF370 0.81 +1.0 402,515 + GFF370 0.81 -0.3 402,515 + GFF370 0.81 -0.2 402,515 + GFF370 0.81 -1.8 402,516 - GFF370 0.81 -0.7 402,516 - GFF370 0.81 +2.2 402,583 + GFF370 0.90 -0.9 402,583 + GFF370 0.90 -1.4 402,584 - GFF370 0.90 +0.3 402,593 + +0.1 402,593 + -0.6 402,594 - -0.2 402,620 + -1.1 402,620 + -0.6 402,621 - -0.9 402,698 + -0.8 402,698 + -0.8 402,699 - -1.8 402,709 + +0.5
Or see this region's nucleotide sequence