Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3685 and GFF3686 are separated by 95 nucleotides GFF3686 and GFF3687 are separated by 122 nucleotides GFF3687 and GFF3688 are separated by 12 nucleotides GFF3688 and GFF3689 are separated by 204 nucleotides
GFF3685 - ATP synthase gamma chain (EC 3.6.3.14), at 184,415 to 185,290
GFF3685
GFF3686 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 185,386 to 185,784
GFF3686
GFF3687 - ATP synthase beta chain (EC 3.6.3.14), at 185,907 to 187,307
GFF3687
GFF3688 - ATP synthase epsilon chain (EC 3.6.3.14), at 187,320 to 187,739
GFF3688
GFF3689 - FIG01198523: hypothetical protein, at 187,944 to 189,146
GFF3689
Position (kb)
185
186
187
188 Strain fitness (log2 ratio)
-1
0
1 at 185.369 kb on + strand at 185.377 kb on + strand at 185.377 kb on + strand at 185.377 kb on + strand at 185.377 kb on + strand at 185.377 kb on + strand at 185.377 kb on + strand at 185.599 kb on + strand, within GFF3686 at 185.599 kb on + strand, within GFF3686 at 185.599 kb on + strand, within GFF3686 at 185.685 kb on + strand, within GFF3686 at 185.685 kb on + strand, within GFF3686 at 187.305 kb on + strand at 187.659 kb on + strand, within GFF3688 at 187.722 kb on + strand at 187.722 kb on + strand at 187.722 kb on + strand at 187.722 kb on + strand at 187.722 kb on + strand at 187.723 kb on - strand at 187.723 kb on - strand at 187.723 kb on - strand at 187.737 kb on + strand at 187.737 kb on + strand at 187.737 kb on + strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.738 kb on - strand at 187.873 kb on + strand at 187.873 kb on + strand at 187.873 kb on + strand at 187.873 kb on + strand at 187.873 kb on + strand at 187.873 kb on + strand at 187.873 kb on + strand at 187.874 kb on - strand at 187.874 kb on - strand at 187.874 kb on - strand at 187.876 kb on + strand at 187.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 185,369 + +0.3 185,377 + -0.3 185,377 + -0.0 185,377 + -0.3 185,377 + +0.5 185,377 + +0.4 185,377 + -0.1 185,599 + GFF3686 0.53 -1.7 185,599 + GFF3686 0.53 -0.2 185,599 + GFF3686 0.53 -0.2 185,685 + GFF3686 0.75 +0.3 185,685 + GFF3686 0.75 -0.1 187,305 + -1.5 187,659 + GFF3688 0.81 -0.2 187,722 + +0.8 187,722 + -0.8 187,722 + +0.4 187,722 + -0.5 187,722 + -0.2 187,723 - +0.1 187,723 - -0.2 187,723 - +1.2 187,737 + +0.1 187,737 + -0.3 187,737 + +0.6 187,738 - +0.0 187,738 - -0.4 187,738 - -1.2 187,738 - +1.2 187,738 - -0.0 187,738 - -0.5 187,738 - +0.5 187,738 - -1.5 187,738 - +0.6 187,873 + +1.2 187,873 + +0.5 187,873 + +0.2 187,873 + +1.0 187,873 + -0.5 187,873 + +1.0 187,873 + +0.1 187,874 - +0.3 187,874 - +0.7 187,874 - +1.3 187,876 + +0.5 187,877 - -0.0
Or see this region's nucleotide sequence