Strain Fitness in Variovorax sp. SCN45 around GFF3680

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3678 and GFF3679 are separated by 157 nucleotidesGFF3679 and GFF3680 are separated by 4 nucleotidesGFF3680 and GFF3681 are separated by 60 nucleotidesGFF3681 and GFF3682 are separated by 75 nucleotidesGFF3682 and GFF3683 are separated by 8 nucleotides GFF3678 - D-alanine aminotransferase (EC 2.6.1.21), at 178,910 to 179,803 GFF3678 GFF3679 - ATP synthase protein I, at 179,961 to 180,446 GFF3679 GFF3680 - ATP synthase F0 sector subunit a (EC 3.6.3.14), at 180,451 to 181,320 GFF3680 GFF3681 - ATP synthase F0 sector subunit c (EC 3.6.3.14), at 181,381 to 181,644 GFF3681 GFF3682 - ATP synthase F0 sector subunit b (EC 3.6.3.14), at 181,720 to 182,190 GFF3682 GFF3683 - ATP synthase delta chain (EC 3.6.3.14), at 182,199 to 182,729 GFF3683 Position (kb) 180 181 182Strain fitness (log2 ratio) -2 -1 0 1 2at 179.511 kb on + strand, within GFF3678at 179.511 kb on + strand, within GFF3678at 179.511 kb on + strand, within GFF3678at 179.512 kb on - strand, within GFF3678at 179.512 kb on - strand, within GFF3678at 179.512 kb on - strand, within GFF3678at 179.664 kb on + strand, within GFF3678at 179.664 kb on + strand, within GFF3678at 179.664 kb on + strand, within GFF3678at 179.665 kb on - strand, within GFF3678at 179.665 kb on - strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.676 kb on + strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.677 kb on - strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.682 kb on + strand, within GFF3678at 179.683 kb on - strand, within GFF3678at 179.683 kb on - strand, within GFF3678at 179.683 kb on - strand, within GFF3678at 179.683 kb on - strand, within GFF3678at 179.683 kb on - strand, within GFF3678at 179.683 kb on - strand, within GFF3678at 179.748 kb on + strandat 179.748 kb on + strandat 179.749 kb on - strandat 179.749 kb on - strandat 179.749 kb on - strandat 179.763 kb on + strandat 179.763 kb on + strandat 179.763 kb on + strandat 179.764 kb on - strandat 179.794 kb on + strandat 179.795 kb on - strandat 179.795 kb on - strandat 179.812 kb on + strandat 179.812 kb on + strandat 179.812 kb on + strandat 179.812 kb on + strandat 179.812 kb on + strandat 179.812 kb on + strandat 179.812 kb on + strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.813 kb on - strandat 179.904 kb on + strandat 179.904 kb on + strandat 179.908 kb on + strandat 179.908 kb on + strandat 179.908 kb on + strandat 179.908 kb on + strandat 179.908 kb on + strandat 179.908 kb on + strandat 179.909 kb on - strandat 179.909 kb on - strandat 179.910 kb on + strandat 179.910 kb on + strandat 180.174 kb on + strand, within GFF3679at 181.662 kb on + strandat 181.662 kb on + strandat 181.662 kb on + strandat 181.662 kb on + strandat 181.662 kb on + strandat 182.191 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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179,511 + GFF3678 0.67 -1.0
179,511 + GFF3678 0.67 +0.5
179,511 + GFF3678 0.67 +0.1
179,512 - GFF3678 0.67 +1.2
179,512 - GFF3678 0.67 +0.8
179,512 - GFF3678 0.67 +1.5
179,664 + GFF3678 0.84 -0.1
179,664 + GFF3678 0.84 +1.4
179,664 + GFF3678 0.84 +0.6
179,665 - GFF3678 0.84 -0.7
179,665 - GFF3678 0.84 -1.5
179,676 + GFF3678 0.86 +1.5
179,676 + GFF3678 0.86 -0.1
179,676 + GFF3678 0.86 -0.3
179,676 + GFF3678 0.86 -0.8
179,676 + GFF3678 0.86 +1.2
179,676 + GFF3678 0.86 -1.1
179,676 + GFF3678 0.86 +0.2
179,676 + GFF3678 0.86 +0.1
179,676 + GFF3678 0.86 -2.3
179,677 - GFF3678 0.86 +0.4
179,677 - GFF3678 0.86 +0.8
179,677 - GFF3678 0.86 +1.3
179,677 - GFF3678 0.86 -0.4
179,677 - GFF3678 0.86 +0.2
179,677 - GFF3678 0.86 -1.7
179,677 - GFF3678 0.86 -0.5
179,677 - GFF3678 0.86 +0.8
179,677 - GFF3678 0.86 +0.0
179,677 - GFF3678 0.86 -1.3
179,677 - GFF3678 0.86 -1.0
179,677 - GFF3678 0.86 -2.2
179,677 - GFF3678 0.86 +0.2
179,682 + GFF3678 0.86 +0.3
179,682 + GFF3678 0.86 -0.0
179,682 + GFF3678 0.86 -0.2
179,682 + GFF3678 0.86 -1.2
179,682 + GFF3678 0.86 -0.8
179,682 + GFF3678 0.86 -0.6
179,682 + GFF3678 0.86 -0.7
179,682 + GFF3678 0.86 +0.4
179,683 - GFF3678 0.86 +0.5
179,683 - GFF3678 0.86 -0.1
179,683 - GFF3678 0.86 -0.0
179,683 - GFF3678 0.86 +0.2
179,683 - GFF3678 0.86 -0.4
179,683 - GFF3678 0.86 -1.2
179,748 + -0.9
179,748 + +2.6
179,749 - -0.1
179,749 - +0.5
179,749 - +0.1
179,763 + -0.0
179,763 + +0.3
179,763 + +0.2
179,764 - -0.1
179,794 + +0.3
179,795 - -0.2
179,795 - +0.2
179,812 + -0.6
179,812 + -0.5
179,812 + +0.6
179,812 + -0.2
179,812 + +0.2
179,812 + +0.9
179,812 + -1.4
179,813 - +2.1
179,813 - +0.0
179,813 - -2.7
179,813 - +0.2
179,813 - -0.2
179,813 - +0.4
179,813 - +0.2
179,813 - -0.6
179,813 - -0.8
179,813 - +0.8
179,904 + -0.2
179,904 + +1.3
179,908 + +0.9
179,908 + -0.2
179,908 + +0.4
179,908 + +0.2
179,908 + -0.2
179,908 + +0.2
179,909 - -0.5
179,909 - +0.2
179,910 + -0.4
179,910 + -0.8
180,174 + GFF3679 0.44 -0.5
181,662 + +0.8
181,662 + +0.5
181,662 + +0.3
181,662 + -0.6
181,662 + +0.2
182,191 + -0.2

Or see this region's nucleotide sequence