Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3634 and GFF3635 are separated by 224 nucleotides GFF3635 and GFF3636 overlap by 1 nucleotides GFF3636 and GFF3637 overlap by 4 nucleotides GFF3637 and GFF3638 are separated by 2 nucleotides
GFF3634 - hypothetical protein, at 146,037 to 146,561
GFF3634
GFF3635 - LSU ribosomal protein L3p (L3e), at 146,786 to 147,466
GFF3635
GFF3636 - LSU ribosomal protein L4p (L1e), at 147,466 to 148,086
GFF3636
GFF3637 - LSU ribosomal protein L23p (L23Ae), at 148,083 to 148,418
GFF3637
GFF3638 - LSU ribosomal protein L2p (L8e), at 148,421 to 149,245
GFF3638
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-2
-1
0
1 at 146.513 kb on + strand at 146.513 kb on + strand at 146.513 kb on + strand at 146.513 kb on + strand at 146.513 kb on + strand at 146.513 kb on + strand at 146.513 kb on + strand at 146.513 kb on + strand at 146.514 kb on - strand at 146.559 kb on + strand at 146.560 kb on - strand at 146.560 kb on - strand at 146.560 kb on - strand at 146.560 kb on - strand at 146.634 kb on + strand at 146.634 kb on + strand at 146.634 kb on + strand at 146.635 kb on - strand at 146.706 kb on + strand at 146.706 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 146,513 + -0.0 146,513 + +0.9 146,513 + +0.5 146,513 + -0.5 146,513 + -0.1 146,513 + +0.8 146,513 + +0.2 146,513 + +0.0 146,514 - +0.2 146,559 + +0.5 146,560 - +1.2 146,560 - -1.2 146,560 - -1.2 146,560 - -1.4 146,634 + +1.0 146,634 + -1.8 146,634 + -0.1 146,635 - +1.5 146,706 + -1.0 146,706 + -0.8
Or see this region's nucleotide sequence