Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3614 and GFF3615 are separated by 3 nucleotides GFF3615 and GFF3616 are separated by 71 nucleotides GFF3616 and GFF3617 are separated by 52 nucleotides GFF3617 and GFF3618 are separated by 44 nucleotides
GFF3614 - UPF0056 inner membrane protein YchE, at 127,926 to 128,579
GFF3614
GFF3615 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 128,583 to 129,329
GFF3615
GFF3616 - 2-hydroxychromene-2-carboxylate isomerase family protein, at 129,401 to 130,006
GFF3616
GFF3617 - Dienelactone hydrolase family protein, at 130,059 to 130,742
GFF3617
GFF3618 - Transcriptional regulator, LysR family, at 130,787 to 131,695
GFF3618
Position (kb) 129
130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 128.479 kb on + strand, within GFF3614 at 128.479 kb on + strand, within GFF3614 at 128.479 kb on + strand, within GFF3614 at 128.480 kb on - strand, within GFF3614 at 128.480 kb on - strand, within GFF3614 at 128.480 kb on - strand, within GFF3614 at 128.492 kb on - strand, within GFF3614 at 128.492 kb on - strand, within GFF3614 at 128.623 kb on + strand at 128.623 kb on + strand at 128.623 kb on + strand at 128.623 kb on + strand at 128.624 kb on - strand at 128.624 kb on - strand at 128.624 kb on - strand at 128.624 kb on - strand at 128.624 kb on - strand at 128.624 kb on - strand at 128.861 kb on - strand, within GFF3615 at 128.869 kb on + strand, within GFF3615 at 128.869 kb on + strand, within GFF3615 at 128.869 kb on + strand, within GFF3615 at 128.869 kb on + strand, within GFF3615 at 128.869 kb on + strand, within GFF3615 at 128.869 kb on + strand, within GFF3615 at 128.869 kb on + strand, within GFF3615 at 128.870 kb on - strand, within GFF3615 at 128.870 kb on - strand, within GFF3615 at 128.870 kb on - strand, within GFF3615 at 128.870 kb on - strand, within GFF3615 at 128.870 kb on - strand, within GFF3615 at 129.232 kb on + strand, within GFF3615 at 129.232 kb on + strand, within GFF3615 at 129.232 kb on + strand, within GFF3615 at 129.232 kb on + strand, within GFF3615 at 129.233 kb on - strand, within GFF3615 at 129.334 kb on + strand at 129.334 kb on + strand at 129.334 kb on + strand at 129.335 kb on - strand at 129.335 kb on - strand at 129.335 kb on - strand at 129.335 kb on - strand at 129.335 kb on - strand at 129.364 kb on - strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.380 kb on + strand at 129.381 kb on - strand at 129.381 kb on - strand at 129.381 kb on - strand at 129.381 kb on - strand at 129.381 kb on - strand at 129.381 kb on - strand at 129.381 kb on - strand at 129.425 kb on + strand at 129.425 kb on + strand at 129.425 kb on + strand at 129.428 kb on + strand at 129.428 kb on + strand at 129.428 kb on + strand at 129.428 kb on + strand at 129.428 kb on + strand at 129.429 kb on - strand at 129.429 kb on - strand at 129.429 kb on - strand at 129.429 kb on - strand at 129.429 kb on - strand at 129.455 kb on + strand at 129.455 kb on + strand at 129.455 kb on + strand at 129.455 kb on + strand at 129.455 kb on + strand at 129.455 kb on + strand at 129.456 kb on - strand at 129.456 kb on - strand at 129.456 kb on - strand at 129.456 kb on - strand at 129.456 kb on - strand at 129.456 kb on - strand at 129.456 kb on - strand at 129.456 kb on - strand at 129.520 kb on + strand, within GFF3616 at 129.593 kb on + strand, within GFF3616 at 129.593 kb on + strand, within GFF3616 at 129.881 kb on + strand, within GFF3616 at 129.882 kb on - strand, within GFF3616 at 129.882 kb on - strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.890 kb on + strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.891 kb on - strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.935 kb on + strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.936 kb on - strand, within GFF3616 at 129.954 kb on + strand at 129.954 kb on + strand at 129.954 kb on + strand at 129.955 kb on - strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.044 kb on + strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.045 kb on - strand at 130.050 kb on + strand at 130.050 kb on + strand at 130.051 kb on - strand at 130.051 kb on - strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.206 kb on + strand, within GFF3617 at 130.206 kb on + strand, within GFF3617 at 130.207 kb on - strand, within GFF3617 at 130.221 kb on + strand, within GFF3617 at 130.221 kb on + strand, within GFF3617 at 130.222 kb on - strand, within GFF3617 at 130.222 kb on - strand, within GFF3617 at 130.222 kb on - strand, within GFF3617 at 130.278 kb on + strand, within GFF3617 at 130.278 kb on + strand, within GFF3617 at 130.278 kb on + strand, within GFF3617 at 130.278 kb on + strand, within GFF3617 at 130.378 kb on - strand, within GFF3617 at 130.413 kb on + strand, within GFF3617 at 130.413 kb on + strand, within GFF3617 at 130.414 kb on - strand, within GFF3617 at 130.483 kb on - strand, within GFF3617 at 130.483 kb on - strand, within GFF3617 at 130.483 kb on - strand, within GFF3617 at 130.485 kb on + strand, within GFF3617 at 130.485 kb on + strand, within GFF3617 at 130.485 kb on + strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.486 kb on - strand, within GFF3617 at 130.632 kb on + strand, within GFF3617 at 130.674 kb on + strand at 130.675 kb on - strand at 130.675 kb on - strand at 130.675 kb on - strand at 130.675 kb on - strand at 130.675 kb on - strand at 130.884 kb on + strand, within GFF3618 at 130.884 kb on + strand, within GFF3618 at 130.885 kb on - strand, within GFF3618 at 130.885 kb on - strand, within GFF3618 at 130.917 kb on + strand, within GFF3618 at 130.918 kb on - strand, within GFF3618 at 130.918 kb on - strand, within GFF3618 at 130.918 kb on - strand, within GFF3618 at 130.918 kb on - strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.983 kb on + strand, within GFF3618 at 130.984 kb on - strand, within GFF3618 at 130.984 kb on - strand, within GFF3618 at 130.984 kb on - strand, within GFF3618 at 130.984 kb on - strand, within GFF3618 at 130.984 kb on - strand, within GFF3618 at 130.984 kb on - strand, within GFF3618
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 128,479 + GFF3614 0.85 -1.8 128,479 + GFF3614 0.85 -0.5 128,479 + GFF3614 0.85 -0.8 128,480 - GFF3614 0.85 -0.3 128,480 - GFF3614 0.85 -0.8 128,480 - GFF3614 0.85 -0.2 128,492 - GFF3614 0.87 -0.7 128,492 - GFF3614 0.87 +0.4 128,623 + +0.2 128,623 + +0.8 128,623 + +0.5 128,623 + -0.9 128,624 - +0.6 128,624 - -0.5 128,624 - -0.3 128,624 - +0.4 128,624 - -1.5 128,624 - +0.4 128,861 - GFF3615 0.37 -0.0 128,869 + GFF3615 0.38 -0.5 128,869 + GFF3615 0.38 +0.4 128,869 + GFF3615 0.38 +0.2 128,869 + GFF3615 0.38 +0.0 128,869 + GFF3615 0.38 -0.2 128,869 + GFF3615 0.38 -0.0 128,869 + GFF3615 0.38 -0.3 128,870 - GFF3615 0.38 +0.1 128,870 - GFF3615 0.38 +0.2 128,870 - GFF3615 0.38 -0.3 128,870 - GFF3615 0.38 -0.3 128,870 - GFF3615 0.38 -0.6 129,232 + GFF3615 0.87 +0.1 129,232 + GFF3615 0.87 +0.1 129,232 + GFF3615 0.87 -0.1 129,232 + GFF3615 0.87 +0.5 129,233 - GFF3615 0.87 +0.6 129,334 + +0.5 129,334 + -0.4 129,334 + +0.8 129,335 - +0.1 129,335 - +0.8 129,335 - +0.1 129,335 - +1.4 129,335 - -0.2 129,364 - -0.2 129,380 + +0.8 129,380 + +0.2 129,380 + -1.8 129,380 + -2.0 129,380 + -0.1 129,380 + -0.0 129,380 + +0.2 129,380 + -0.3 129,380 + +0.6 129,380 + -0.1 129,381 - -0.3 129,381 - -0.4 129,381 - -0.2 129,381 - +0.4 129,381 - +0.5 129,381 - -0.2 129,381 - +0.1 129,425 + -0.2 129,425 + +0.3 129,425 + -0.7 129,428 + -0.1 129,428 + -0.9 129,428 + -0.2 129,428 + -2.1 129,428 + -1.2 129,429 - -0.2 129,429 - -0.5 129,429 - +0.1 129,429 - +1.8 129,429 - -0.3 129,455 + -1.1 129,455 + -0.6 129,455 + -0.2 129,455 + +0.2 129,455 + +1.1 129,455 + +0.0 129,456 - +0.2 129,456 - -1.4 129,456 - -1.2 129,456 - +0.8 129,456 - +0.9 129,456 - +0.3 129,456 - +0.1 129,456 - -0.3 129,520 + GFF3616 0.20 +0.2 129,593 + GFF3616 0.32 -0.3 129,593 + GFF3616 0.32 +0.5 129,881 + GFF3616 0.79 -0.1 129,882 - GFF3616 0.79 -0.8 129,882 - GFF3616 0.79 +0.1 129,890 + GFF3616 0.81 +0.5 129,890 + GFF3616 0.81 -0.2 129,890 + GFF3616 0.81 -1.1 129,890 + GFF3616 0.81 +0.1 129,890 + GFF3616 0.81 -0.7 129,890 + GFF3616 0.81 -0.5 129,890 + GFF3616 0.81 -0.1 129,890 + GFF3616 0.81 +0.0 129,890 + GFF3616 0.81 +0.6 129,890 + GFF3616 0.81 +0.2 129,890 + GFF3616 0.81 -0.4 129,890 + GFF3616 0.81 -0.9 129,890 + GFF3616 0.81 -0.2 129,890 + GFF3616 0.81 +1.0 129,890 + GFF3616 0.81 +0.4 129,891 - GFF3616 0.81 +2.0 129,891 - GFF3616 0.81 -0.8 129,891 - GFF3616 0.81 +0.9 129,891 - GFF3616 0.81 -0.6 129,891 - GFF3616 0.81 +0.5 129,891 - GFF3616 0.81 -0.2 129,891 - GFF3616 0.81 +0.6 129,891 - GFF3616 0.81 +0.0 129,891 - GFF3616 0.81 -0.2 129,935 + GFF3616 0.88 -0.1 129,935 + GFF3616 0.88 +0.0 129,935 + GFF3616 0.88 -0.4 129,935 + GFF3616 0.88 +1.0 129,935 + GFF3616 0.88 +0.8 129,935 + GFF3616 0.88 +0.5 129,935 + GFF3616 0.88 -1.2 129,935 + GFF3616 0.88 +0.4 129,935 + GFF3616 0.88 +1.4 129,935 + GFF3616 0.88 -0.7 129,935 + GFF3616 0.88 +0.6 129,935 + GFF3616 0.88 -0.4 129,935 + GFF3616 0.88 +0.8 129,935 + GFF3616 0.88 -0.6 129,935 + GFF3616 0.88 -0.9 129,936 - GFF3616 0.88 -0.2 129,936 - GFF3616 0.88 -0.5 129,936 - GFF3616 0.88 -0.7 129,936 - GFF3616 0.88 +0.7 129,936 - GFF3616 0.88 +2.2 129,936 - GFF3616 0.88 -0.5 129,936 - GFF3616 0.88 +0.2 129,936 - GFF3616 0.88 +1.7 129,936 - GFF3616 0.88 -0.1 129,936 - GFF3616 0.88 -0.6 129,936 - GFF3616 0.88 +0.6 129,936 - GFF3616 0.88 +1.4 129,936 - GFF3616 0.88 -0.0 129,936 - GFF3616 0.88 +0.5 129,936 - GFF3616 0.88 +1.1 129,936 - GFF3616 0.88 -0.2 129,954 + +1.0 129,954 + -0.7 129,954 + -1.0 129,955 - -0.1 130,044 + -0.9 130,044 + -0.6 130,044 + -0.3 130,044 + -0.0 130,044 + +0.6 130,044 + -1.7 130,044 + +0.2 130,044 + -0.1 130,044 + +0.2 130,044 + +2.4 130,044 + +0.6 130,044 + -0.0 130,044 + -0.1 130,044 + -0.5 130,044 + +0.2 130,044 + -3.2 130,044 + +0.3 130,045 - +0.5 130,045 - +0.8 130,045 - -1.1 130,045 - -0.6 130,045 - -1.7 130,045 - -0.5 130,045 - +2.8 130,045 - +0.1 130,045 - -0.4 130,045 - -0.3 130,045 - -0.6 130,045 - +2.0 130,045 - -0.4 130,045 - -1.0 130,045 - +0.2 130,045 - +0.6 130,045 - -1.8 130,045 - +0.0 130,050 + +0.1 130,050 + +0.6 130,051 - -0.1 130,051 - +2.4 130,110 + +0.7 130,110 + -0.2 130,110 + -0.3 130,110 + -0.3 130,111 - +0.3 130,111 - -1.2 130,111 - -0.4 130,206 + GFF3617 0.21 -0.4 130,206 + GFF3617 0.21 +0.2 130,207 - GFF3617 0.22 +0.0 130,221 + GFF3617 0.24 +0.9 130,221 + GFF3617 0.24 +0.5 130,222 - GFF3617 0.24 +0.8 130,222 - GFF3617 0.24 -0.8 130,222 - GFF3617 0.24 +0.4 130,278 + GFF3617 0.32 +2.1 130,278 + GFF3617 0.32 -0.4 130,278 + GFF3617 0.32 -0.4 130,278 + GFF3617 0.32 -0.2 130,378 - GFF3617 0.47 -0.3 130,413 + GFF3617 0.52 -0.5 130,413 + GFF3617 0.52 -0.6 130,414 - GFF3617 0.52 -1.4 130,483 - GFF3617 0.62 +0.3 130,483 - GFF3617 0.62 -0.2 130,483 - GFF3617 0.62 -0.4 130,485 + GFF3617 0.62 -0.1 130,485 + GFF3617 0.62 +0.2 130,485 + GFF3617 0.62 +1.8 130,486 - GFF3617 0.62 +0.4 130,486 - GFF3617 0.62 +0.6 130,486 - GFF3617 0.62 -0.3 130,486 - GFF3617 0.62 -0.4 130,486 - GFF3617 0.62 +0.9 130,486 - GFF3617 0.62 -0.4 130,486 - GFF3617 0.62 -0.0 130,486 - GFF3617 0.62 -0.1 130,486 - GFF3617 0.62 -1.8 130,486 - GFF3617 0.62 +0.2 130,632 + GFF3617 0.84 -0.0 130,674 + -0.8 130,675 - +0.5 130,675 - -0.8 130,675 - +0.0 130,675 - -0.3 130,675 - -0.8 130,884 + GFF3618 0.11 +0.2 130,884 + GFF3618 0.11 -0.2 130,885 - GFF3618 0.11 +0.1 130,885 - GFF3618 0.11 +0.6 130,917 + GFF3618 0.14 -0.5 130,918 - GFF3618 0.14 -0.3 130,918 - GFF3618 0.14 -0.0 130,918 - GFF3618 0.14 -0.0 130,918 - GFF3618 0.14 +0.6 130,983 + GFF3618 0.22 -0.1 130,983 + GFF3618 0.22 -0.7 130,983 + GFF3618 0.22 +0.1 130,983 + GFF3618 0.22 -0.9 130,983 + GFF3618 0.22 -0.4 130,983 + GFF3618 0.22 +0.9 130,983 + GFF3618 0.22 -0.3 130,983 + GFF3618 0.22 -0.3 130,983 + GFF3618 0.22 +0.0 130,983 + GFF3618 0.22 -0.6 130,984 - GFF3618 0.22 -1.1 130,984 - GFF3618 0.22 -0.4 130,984 - GFF3618 0.22 -0.8 130,984 - GFF3618 0.22 -0.8 130,984 - GFF3618 0.22 -1.6 130,984 - GFF3618 0.22 -0.5
Or see this region's nucleotide sequence