Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF357 and GFF358 are separated by 137 nucleotides GFF358 and GFF359 are separated by 91 nucleotides GFF359 and GFF360 overlap by 4 nucleotides
GFF357 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 387,889 to 388,572
GFF357
GFF358 - hypothetical protein, at 388,710 to 389,075
GFF358
GFF359 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 389,167 to 390,174
GFF359
GFF360 - Putative transporter, at 390,171 to 391,328
GFF360
Position (kb) 389
390
391 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.258 kb on + strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.259 kb on - strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.261 kb on + strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.262 kb on - strand, within GFF357 at 388.297 kb on + strand, within GFF357 at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.570 kb on + strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.571 kb on - strand at 388.611 kb on + strand at 388.626 kb on + strand at 388.626 kb on + strand at 388.627 kb on - strand at 388.627 kb on - strand at 388.627 kb on - strand at 388.627 kb on - strand at 388.692 kb on + strand at 388.693 kb on - strand at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.842 kb on + strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 388.843 kb on - strand, within GFF358 at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.172 kb on + strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.173 kb on - strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.191 kb on + strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.192 kb on - strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.239 kb on + strand at 389.240 kb on - strand at 389.240 kb on - strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.254 kb on + strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.255 kb on - strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.256 kb on + strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.257 kb on - strand at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.533 kb on + strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.534 kb on - strand, within GFF359 at 389.650 kb on + strand, within GFF359 at 389.681 kb on - strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.055 kb on + strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.056 kb on - strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.073 kb on + strand, within GFF359 at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand at 390.074 kb on - strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.082 kb on + strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.083 kb on - strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.115 kb on + strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.116 kb on - strand at 390.335 kb on - strand, within GFF360 at 390.335 kb on - strand, within GFF360 at 390.417 kb on + strand, within GFF360 at 390.417 kb on + strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.418 kb on - strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.646 kb on + strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.647 kb on - strand, within GFF360 at 390.846 kb on + strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 390.847 kb on - strand, within GFF360 at 390.847 kb on - strand, within GFF360
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 388,258 + GFF357 0.54 -0.8 388,258 + GFF357 0.54 +0.4 388,258 + GFF357 0.54 +0.2 388,258 + GFF357 0.54 -0.5 388,258 + GFF357 0.54 -0.8 388,258 + GFF357 0.54 -0.5 388,258 + GFF357 0.54 +0.2 388,258 + GFF357 0.54 +0.8 388,258 + GFF357 0.54 -0.9 388,258 + GFF357 0.54 +0.2 388,258 + GFF357 0.54 -0.7 388,258 + GFF357 0.54 -2.1 388,259 - GFF357 0.54 -0.6 388,259 - GFF357 0.54 -0.7 388,259 - GFF357 0.54 -0.1 388,259 - GFF357 0.54 +1.2 388,259 - GFF357 0.54 +0.3 388,259 - GFF357 0.54 -0.8 388,259 - GFF357 0.54 -0.4 388,259 - GFF357 0.54 -0.7 388,259 - GFF357 0.54 -0.6 388,259 - GFF357 0.54 +0.0 388,259 - GFF357 0.54 -1.3 388,259 - GFF357 0.54 +1.2 388,259 - GFF357 0.54 +0.1 388,259 - GFF357 0.54 -0.3 388,259 - GFF357 0.54 +0.5 388,259 - GFF357 0.54 -0.1 388,259 - GFF357 0.54 -0.1 388,259 - GFF357 0.54 -0.3 388,259 - GFF357 0.54 +0.6 388,259 - GFF357 0.54 +0.2 388,259 - GFF357 0.54 +0.7 388,259 - GFF357 0.54 -0.3 388,259 - GFF357 0.54 +0.5 388,261 + GFF357 0.54 +0.5 388,261 + GFF357 0.54 -0.4 388,261 + GFF357 0.54 -0.5 388,261 + GFF357 0.54 +0.9 388,261 + GFF357 0.54 +0.7 388,261 + GFF357 0.54 +0.9 388,261 + GFF357 0.54 -0.7 388,261 + GFF357 0.54 +0.2 388,262 - GFF357 0.55 +0.1 388,262 - GFF357 0.55 -0.6 388,262 - GFF357 0.55 +0.5 388,262 - GFF357 0.55 -0.1 388,262 - GFF357 0.55 -0.7 388,262 - GFF357 0.55 +0.8 388,262 - GFF357 0.55 -0.9 388,262 - GFF357 0.55 -0.5 388,297 + GFF357 0.60 -0.4 388,570 + +0.1 388,570 + +0.3 388,570 + +2.8 388,570 + +0.7 388,570 + +0.2 388,570 + -0.1 388,570 + -1.4 388,570 + -0.4 388,570 + +0.6 388,570 + -0.0 388,570 + +0.3 388,570 + -0.0 388,570 + -0.1 388,570 + +0.7 388,570 + -0.9 388,570 + +0.4 388,571 - -0.3 388,571 - -0.7 388,571 - -0.2 388,571 - -0.8 388,571 - -0.0 388,571 - +0.7 388,571 - +1.8 388,571 - +1.7 388,571 - -0.2 388,571 - -0.0 388,571 - +0.4 388,571 - +0.4 388,571 - -0.1 388,611 + -0.6 388,626 + -1.4 388,626 + -0.1 388,627 - +0.4 388,627 - +0.8 388,627 - +0.2 388,627 - -1.6 388,692 + -0.4 388,693 - +0.7 388,842 + GFF358 0.36 +0.1 388,842 + GFF358 0.36 +0.8 388,842 + GFF358 0.36 -0.9 388,842 + GFF358 0.36 -0.5 388,842 + GFF358 0.36 -0.8 388,842 + GFF358 0.36 -0.1 388,842 + GFF358 0.36 -0.4 388,842 + GFF358 0.36 -1.0 388,842 + GFF358 0.36 +0.0 388,843 - GFF358 0.36 -0.8 388,843 - GFF358 0.36 -1.8 388,843 - GFF358 0.36 -1.1 388,843 - GFF358 0.36 -0.6 388,843 - GFF358 0.36 -0.0 389,172 + -0.0 389,172 + -0.2 389,172 + -0.2 389,172 + -0.6 389,172 + +0.2 389,172 + -0.7 389,173 - +2.2 389,173 - -0.4 389,173 - -0.7 389,173 - -0.9 389,173 - -0.1 389,173 - +0.4 389,173 - -0.7 389,173 - -0.9 389,173 - +0.1 389,191 + +0.2 389,191 + +0.6 389,191 + -0.6 389,192 - -0.9 389,192 - -0.6 389,192 - +0.7 389,239 + -2.3 389,239 + -0.2 389,239 + -1.0 389,240 - +0.0 389,240 - +0.4 389,254 + -0.1 389,254 + -1.5 389,254 + -0.3 389,254 + +0.2 389,254 + +0.2 389,254 + +1.2 389,254 + -0.0 389,254 + -0.7 389,255 - +0.6 389,255 - +0.5 389,255 - +0.2 389,255 - +0.6 389,255 - -0.1 389,255 - -1.5 389,255 - +1.3 389,255 - -0.2 389,256 + +0.2 389,256 + -0.1 389,256 + +0.1 389,256 + +0.4 389,256 + -0.3 389,256 + -0.9 389,256 + +0.2 389,256 + +1.5 389,257 - -1.0 389,257 - -1.0 389,257 - +0.9 389,257 - +0.5 389,257 - +0.6 389,257 - +0.1 389,257 - +0.5 389,257 - +1.4 389,257 - -1.5 389,257 - -0.2 389,533 + GFF359 0.36 -0.8 389,533 + GFF359 0.36 -0.8 389,533 + GFF359 0.36 -1.1 389,533 + GFF359 0.36 -1.0 389,533 + GFF359 0.36 -2.0 389,533 + GFF359 0.36 -0.1 389,533 + GFF359 0.36 +0.4 389,533 + GFF359 0.36 -0.5 389,533 + GFF359 0.36 -0.6 389,533 + GFF359 0.36 +0.2 389,533 + GFF359 0.36 +0.2 389,533 + GFF359 0.36 +1.5 389,534 - GFF359 0.36 -0.7 389,534 - GFF359 0.36 -1.0 389,534 - GFF359 0.36 -1.5 389,534 - GFF359 0.36 -0.7 389,534 - GFF359 0.36 -2.0 389,534 - GFF359 0.36 -0.1 389,534 - GFF359 0.36 -0.3 389,534 - GFF359 0.36 -0.4 389,534 - GFF359 0.36 +0.3 389,534 - GFF359 0.36 -0.6 389,534 - GFF359 0.36 +0.2 389,650 + GFF359 0.48 -1.8 389,681 - GFF359 0.51 +0.4 390,055 + GFF359 0.88 -0.5 390,055 + GFF359 0.88 -0.6 390,055 + GFF359 0.88 -1.3 390,056 - GFF359 0.88 -0.2 390,056 - GFF359 0.88 -1.1 390,056 - GFF359 0.88 -0.0 390,056 - GFF359 0.88 +0.6 390,073 + GFF359 0.90 -0.4 390,073 + GFF359 0.90 +0.4 390,074 - +1.2 390,074 - -0.7 390,074 - -0.5 390,074 - -0.3 390,082 + -0.2 390,082 + +2.4 390,082 + -1.1 390,082 + -0.2 390,083 - -0.7 390,083 - +0.1 390,083 - +0.1 390,083 - +0.1 390,083 - -0.1 390,083 - +0.3 390,083 - -1.2 390,115 + -0.2 390,115 + -0.6 390,115 + -0.6 390,115 + -0.3 390,115 + +0.3 390,115 + -0.6 390,115 + -0.1 390,115 + +1.6 390,115 + +1.2 390,115 + +0.2 390,115 + -1.3 390,115 + -1.4 390,116 - -1.5 390,116 - +0.2 390,116 - -0.6 390,116 - -0.0 390,116 - -1.8 390,116 - -0.9 390,116 - -0.1 390,116 - -0.5 390,116 - +0.4 390,116 - -0.8 390,116 - -2.8 390,116 - -0.9 390,116 - -0.3 390,335 - GFF360 0.14 -1.1 390,335 - GFF360 0.14 -0.4 390,417 + GFF360 0.21 -0.3 390,417 + GFF360 0.21 +0.9 390,418 - GFF360 0.21 -0.2 390,418 - GFF360 0.21 +0.3 390,418 - GFF360 0.21 -0.4 390,646 + GFF360 0.41 -0.2 390,646 + GFF360 0.41 +0.4 390,646 + GFF360 0.41 +0.6 390,646 + GFF360 0.41 -0.7 390,646 + GFF360 0.41 -1.1 390,646 + GFF360 0.41 -1.5 390,647 - GFF360 0.41 -0.3 390,647 - GFF360 0.41 -0.1 390,647 - GFF360 0.41 -0.5 390,647 - GFF360 0.41 +0.1 390,647 - GFF360 0.41 +0.2 390,647 - GFF360 0.41 -0.4 390,647 - GFF360 0.41 -0.4 390,647 - GFF360 0.41 +0.6 390,647 - GFF360 0.41 -1.1 390,647 - GFF360 0.41 +0.2 390,846 + GFF360 0.58 -0.0 390,847 - GFF360 0.58 +0.2 390,847 - GFF360 0.58 -0.8 390,847 - GFF360 0.58 -0.4
Or see this region's nucleotide sequence