Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3578 and GFF3579 overlap by 4 nucleotides GFF3579 and GFF3580 overlap by 43 nucleotides GFF3580 and GFF3581 are separated by 82 nucleotides GFF3581 and GFF3582 are separated by 31 nucleotides
GFF3578 - Glutathione S-transferase, unnamed subgroup (EC 2.5.1.18), at 89,558 to 90,217
GFF3578
GFF3579 - probable iron-sulfur binding protein YPO1417, at 90,214 to 91,203
GFF3579
GFF3580 - COG2124: Cytochrome P450, at 91,161 to 92,330
GFF3580
GFF3581 - Transcriptional regulator, ArsR family, at 92,413 to 93,105
GFF3581
GFF3582 - Tripartite tricarboxylate transporter TctC family, at 93,137 to 94,147
GFF3582
Position (kb) 91
92
93 Strain fitness (log2 ratio) -1
0
1 at 90.351 kb on - strand, within GFF3579 at 90.640 kb on + strand, within GFF3579 at 90.641 kb on - strand, within GFF3579 at 90.830 kb on + strand, within GFF3579 at 90.830 kb on + strand, within GFF3579 at 90.830 kb on + strand, within GFF3579 at 90.831 kb on - strand, within GFF3579 at 90.831 kb on - strand, within GFF3579 at 92.245 kb on + strand at 92.245 kb on + strand at 92.246 kb on - strand at 92.246 kb on - strand at 92.254 kb on + strand at 92.401 kb on + strand at 92.402 kb on - strand at 92.765 kb on - strand, within GFF3581 at 92.765 kb on - strand, within GFF3581 at 92.765 kb on - strand, within GFF3581 at 92.765 kb on - strand, within GFF3581 at 93.159 kb on + strand at 93.159 kb on + strand at 93.160 kb on - strand at 93.160 kb on - strand at 93.160 kb on - strand at 93.160 kb on - strand at 93.160 kb on - strand at 93.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 90,351 - GFF3579 0.14 -0.2 90,640 + GFF3579 0.43 +0.1 90,641 - GFF3579 0.43 -0.2 90,830 + GFF3579 0.62 +0.0 90,830 + GFF3579 0.62 +0.8 90,830 + GFF3579 0.62 +0.1 90,831 - GFF3579 0.62 -0.9 90,831 - GFF3579 0.62 +0.2 92,245 + -0.2 92,245 + +0.8 92,246 - -0.7 92,246 - +0.0 92,254 + +0.1 92,401 + +0.0 92,402 - -0.2 92,765 - GFF3581 0.51 -1.6 92,765 - GFF3581 0.51 -1.2 92,765 - GFF3581 0.51 +0.1 92,765 - GFF3581 0.51 -0.1 93,159 + -0.4 93,159 + -0.2 93,160 - +0.8 93,160 - -0.5 93,160 - -0.1 93,160 - +1.2 93,160 - -0.8 93,228 + +0.3
Or see this region's nucleotide sequence