Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF353 and GFF354 overlap by 4 nucleotides GFF354 and GFF355 are separated by 137 nucleotides GFF355 and GFF356 are separated by 34 nucleotides
GFF353 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 385,072 to 385,254
GFF353
GFF354 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 385,251 to 386,024
GFF354
GFF355 - Adenylate kinase (EC 2.7.4.3), at 386,162 to 386,818
GFF355
GFF356 - L-asparaginase (EC 3.5.1.1), at 386,853 to 387,815
GFF356
Position (kb)
386
387 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 386.079 kb on + strand at 386.079 kb on + strand at 386.080 kb on - strand at 386.080 kb on - strand at 386.080 kb on - strand at 386.080 kb on - strand at 386.080 kb on - strand at 387.250 kb on + strand, within GFF356 at 387.250 kb on + strand, within GFF356 at 387.250 kb on + strand, within GFF356 at 387.250 kb on + strand, within GFF356 at 387.250 kb on + strand, within GFF356 at 387.250 kb on + strand, within GFF356 at 387.250 kb on + strand, within GFF356 at 387.250 kb on + strand, within GFF356 at 387.251 kb on - strand, within GFF356 at 387.251 kb on - strand, within GFF356 at 387.251 kb on - strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.295 kb on + strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.296 kb on - strand, within GFF356 at 387.715 kb on + strand, within GFF356 at 387.716 kb on - strand, within GFF356 at 387.716 kb on - strand, within GFF356
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 386,079 + -0.2 386,079 + -0.4 386,080 - +2.2 386,080 - -0.7 386,080 - +0.8 386,080 - +0.5 386,080 - +0.4 387,250 + GFF356 0.41 +0.3 387,250 + GFF356 0.41 +0.6 387,250 + GFF356 0.41 +0.8 387,250 + GFF356 0.41 -0.3 387,250 + GFF356 0.41 -0.5 387,250 + GFF356 0.41 -1.4 387,250 + GFF356 0.41 -0.3 387,250 + GFF356 0.41 -0.5 387,251 - GFF356 0.41 -0.8 387,251 - GFF356 0.41 -0.1 387,251 - GFF356 0.41 -0.1 387,295 + GFF356 0.46 +0.8 387,295 + GFF356 0.46 -0.4 387,295 + GFF356 0.46 -0.7 387,295 + GFF356 0.46 -0.1 387,295 + GFF356 0.46 +0.7 387,295 + GFF356 0.46 -0.8 387,295 + GFF356 0.46 +0.7 387,295 + GFF356 0.46 +0.0 387,295 + GFF356 0.46 +0.9 387,296 - GFF356 0.46 +0.5 387,296 - GFF356 0.46 -0.5 387,296 - GFF356 0.46 -0.7 387,296 - GFF356 0.46 +0.2 387,296 - GFF356 0.46 +0.3 387,296 - GFF356 0.46 +0.8 387,296 - GFF356 0.46 -0.5 387,296 - GFF356 0.46 -2.3 387,715 + GFF356 0.90 -0.1 387,716 - GFF356 0.90 -0.4 387,716 - GFF356 0.90 -0.4
Or see this region's nucleotide sequence