Strain Fitness in Variovorax sp. SCN45 around GFF353

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF352 and GFF353 overlap by 20 nucleotidesGFF353 and GFF354 overlap by 4 nucleotidesGFF354 and GFF355 are separated by 137 nucleotides GFF352 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 384,063 to 385,091 GFF352 GFF353 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 385,072 to 385,254 GFF353 GFF354 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 385,251 to 386,024 GFF354 GFF355 - Adenylate kinase (EC 2.7.4.3), at 386,162 to 386,818 GFF355 Position (kb) 385 386Strain fitness (log2 ratio) -1 0 1 2at 385.060 kb on + strandat 385.060 kb on + strandat 385.060 kb on + strandat 385.060 kb on + strandat 385.060 kb on + strandat 385.060 kb on + strandat 385.060 kb on + strandat 385.060 kb on + strandat 385.061 kb on - strandat 385.061 kb on - strandat 385.061 kb on - strandat 385.061 kb on - strandat 385.061 kb on - strandat 385.061 kb on - strandat 385.061 kb on - strandat 386.079 kb on + strandat 386.079 kb on + strandat 386.080 kb on - strandat 386.080 kb on - strandat 386.080 kb on - strandat 386.080 kb on - strandat 386.080 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
remove
385,060 + -0.3
385,060 + -1.3
385,060 + -0.8
385,060 + +0.6
385,060 + -0.5
385,060 + +0.4
385,060 + -0.6
385,060 + -0.3
385,061 - +0.4
385,061 - -1.4
385,061 - -1.6
385,061 - -0.1
385,061 - -0.8
385,061 - +1.0
385,061 - -1.4
386,079 + -0.2
386,079 + -0.4
386,080 - +2.2
386,080 - -0.7
386,080 - +0.8
386,080 - +0.5
386,080 - +0.4

Or see this region's nucleotide sequence