Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF350 and GFF351 are separated by 19 nucleotides GFF351 and GFF352 are separated by 0 nucleotides GFF352 and GFF353 overlap by 20 nucleotides GFF353 and GFF354 overlap by 4 nucleotides
GFF350 - Ferric siderophore transport system, biopolymer transport protein ExbB, at 382,985 to 383,617
GFF350
GFF351 - Biopolymer transport protein ExbD/TolR, at 383,637 to 384,062
GFF351
GFF352 - Tetraacyldisaccharide 4'-kinase (EC 2.7.1.130), at 384,063 to 385,091
GFF352
GFF353 - FIG002473: Protein YcaR in KDO2-Lipid A biosynthesis cluster, at 385,072 to 385,254
GFF353
GFF354 - 3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38), at 385,251 to 386,024
GFF354
Position (kb) 384
385
386 Strain fitness (log2 ratio) -2
-1
0
1
2 at 383.064 kb on + strand, within GFF350 at 383.064 kb on + strand, within GFF350 at 383.064 kb on + strand, within GFF350 at 383.064 kb on + strand, within GFF350 at 383.064 kb on + strand, within GFF350 at 383.064 kb on + strand, within GFF350 at 383.064 kb on + strand, within GFF350 at 383.064 kb on + strand, within GFF350 at 383.065 kb on - strand, within GFF350 at 383.065 kb on - strand, within GFF350 at 383.065 kb on - strand, within GFF350 at 383.065 kb on - strand, within GFF350 at 383.065 kb on - strand, within GFF350 at 383.097 kb on + strand, within GFF350 at 383.098 kb on - strand, within GFF350 at 383.312 kb on + strand, within GFF350 at 383.312 kb on + strand, within GFF350 at 383.474 kb on + strand, within GFF350 at 383.474 kb on + strand, within GFF350 at 383.474 kb on + strand, within GFF350 at 383.474 kb on + strand, within GFF350 at 383.474 kb on + strand, within GFF350 at 383.474 kb on + strand, within GFF350 at 383.474 kb on + strand, within GFF350 at 383.528 kb on + strand, within GFF350 at 383.528 kb on + strand, within GFF350 at 383.552 kb on + strand, within GFF350 at 383.552 kb on + strand, within GFF350 at 383.739 kb on + strand, within GFF351 at 385.060 kb on + strand at 385.060 kb on + strand at 385.060 kb on + strand at 385.060 kb on + strand at 385.060 kb on + strand at 385.060 kb on + strand at 385.060 kb on + strand at 385.060 kb on + strand at 385.061 kb on - strand at 385.061 kb on - strand at 385.061 kb on - strand at 385.061 kb on - strand at 385.061 kb on - strand at 385.061 kb on - strand at 385.061 kb on - strand at 386.079 kb on + strand at 386.079 kb on + strand at 386.080 kb on - strand at 386.080 kb on - strand at 386.080 kb on - strand at 386.080 kb on - strand at 386.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 383,064 + GFF350 0.12 +0.2 383,064 + GFF350 0.12 +0.5 383,064 + GFF350 0.12 -0.4 383,064 + GFF350 0.12 -0.5 383,064 + GFF350 0.12 -0.7 383,064 + GFF350 0.12 -1.3 383,064 + GFF350 0.12 +1.8 383,064 + GFF350 0.12 +0.2 383,065 - GFF350 0.13 +0.8 383,065 - GFF350 0.13 +0.2 383,065 - GFF350 0.13 -0.8 383,065 - GFF350 0.13 +0.6 383,065 - GFF350 0.13 -0.4 383,097 + GFF350 0.18 -0.9 383,098 - GFF350 0.18 -0.4 383,312 + GFF350 0.52 +0.9 383,312 + GFF350 0.52 -0.8 383,474 + GFF350 0.77 -1.0 383,474 + GFF350 0.77 +0.6 383,474 + GFF350 0.77 +0.1 383,474 + GFF350 0.77 +0.4 383,474 + GFF350 0.77 -0.8 383,474 + GFF350 0.77 -0.4 383,474 + GFF350 0.77 -1.0 383,528 + GFF350 0.86 -0.1 383,528 + GFF350 0.86 +2.2 383,552 + GFF350 0.90 -1.8 383,552 + GFF350 0.90 +1.6 383,739 + GFF351 0.24 +0.5 385,060 + -0.3 385,060 + -1.3 385,060 + -0.8 385,060 + +0.6 385,060 + -0.5 385,060 + +0.4 385,060 + -0.6 385,060 + -0.3 385,061 - +0.4 385,061 - -1.4 385,061 - -1.6 385,061 - -0.1 385,061 - -0.8 385,061 - +1.0 385,061 - -1.4 386,079 + -0.2 386,079 + -0.4 386,080 - +2.2 386,080 - -0.7 386,080 - +0.8 386,080 - +0.5 386,080 - +0.4
Or see this region's nucleotide sequence