Strain Fitness in Variovorax sp. SCN45 around GFF3453

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3452 and GFF3453 are separated by 64 nucleotidesGFF3453 and GFF3454 are separated by 124 nucleotides GFF3452 - hypothetical protein, at 273,925 to 275,598 GFF3452 GFF3453 - hypothetical protein, at 275,663 to 275,857 GFF3453 GFF3454 - Cys regulon transcriptional activator CysB, at 275,982 to 276,872 GFF3454 Position (kb) 275 276Strain fitness (log2 ratio) -2 -1 0 1at 274.811 kb on + strand, within GFF3452at 274.812 kb on - strand, within GFF3452at 274.854 kb on - strand, within GFF3452at 275.015 kb on - strand, within GFF3452at 275.904 kb on - strandat 275.949 kb on + strandat 276.378 kb on + strand, within GFF3454at 276.378 kb on + strand, within GFF3454at 276.379 kb on - strand, within GFF3454at 276.588 kb on + strand, within GFF3454at 276.588 kb on + strand, within GFF3454at 276.588 kb on + strand, within GFF3454at 276.588 kb on + strand, within GFF3454at 276.588 kb on + strand, within GFF3454at 276.589 kb on - strand, within GFF3454at 276.589 kb on - strand, within GFF3454at 276.713 kb on - strand, within GFF3454

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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274,811 + GFF3452 0.53 -0.6
274,812 - GFF3452 0.53 -0.8
274,854 - GFF3452 0.55 -0.1
275,015 - GFF3452 0.65 -0.1
275,904 - -0.1
275,949 + +0.1
276,378 + GFF3454 0.44 +0.2
276,378 + GFF3454 0.44 -0.1
276,379 - GFF3454 0.45 +0.3
276,588 + GFF3454 0.68 -0.6
276,588 + GFF3454 0.68 +0.3
276,588 + GFF3454 0.68 -2.1
276,588 + GFF3454 0.68 -2.2
276,588 + GFF3454 0.68 +0.4
276,589 - GFF3454 0.68 -1.2
276,589 - GFF3454 0.68 +0.8
276,713 - GFF3454 0.82 -0.3

Or see this region's nucleotide sequence