Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3415 and GFF3416 are separated by 41 nucleotides GFF3416 and GFF3417 overlap by 4 nucleotides GFF3417 and GFF3418 overlap by 8 nucleotides GFF3418 and GFF3419 are separated by 74 nucleotides
GFF3415 - Cyclase, at 239,335 to 240,141
GFF3415
GFF3416 - Alkanesulfonates transport system permease protein, at 240,183 to 240,962
GFF3416
GFF3417 - Alkanesulfonates transport system permease protein, at 240,959 to 241,729
GFF3417
GFF3418 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 241,722 to 242,501
GFF3418
GFF3419 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 242,576 to 243,535
GFF3419
Position (kb) 240
241
242 Strain fitness (log2 ratio) -1
0
1
2 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.036 kb on - strand, within GFF3415 at 240.036 kb on - strand, within GFF3415 at 240.056 kb on + strand, within GFF3415 at 240.448 kb on + strand, within GFF3416 at 240.449 kb on - strand, within GFF3416 at 240.836 kb on + strand, within GFF3416 at 240.946 kb on + strand at 241.653 kb on + strand at 241.654 kb on - strand at 241.670 kb on + strand at 241.775 kb on - strand at 241.900 kb on + strand, within GFF3418 at 241.900 kb on + strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.940 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 240,035 + GFF3415 0.87 -0.3 240,035 + GFF3415 0.87 -0.3 240,035 + GFF3415 0.87 -1.7 240,035 + GFF3415 0.87 +0.5 240,035 + GFF3415 0.87 +0.9 240,036 - GFF3415 0.87 +0.3 240,036 - GFF3415 0.87 -0.1 240,056 + GFF3415 0.89 -0.5 240,448 + GFF3416 0.34 -0.1 240,449 - GFF3416 0.34 +0.2 240,836 + GFF3416 0.84 +1.8 240,946 + -0.3 241,653 + -0.3 241,654 - +0.1 241,670 + -1.2 241,775 - -0.2 241,900 + GFF3418 0.23 -1.3 241,900 + GFF3418 0.23 -1.0 241,901 - GFF3418 0.23 +0.8 241,901 - GFF3418 0.23 +2.0 241,940 + GFF3418 0.28 +0.3 242,334 + GFF3418 0.78 -0.1 242,334 + GFF3418 0.78 -0.1
Or see this region's nucleotide sequence