Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3363 and GFF3364 are separated by 98 nucleotides GFF3364 and GFF3365 overlap by 23 nucleotides GFF3365 and GFF3366 are separated by 3 nucleotides
GFF3363 - BUG/TctC family periplasmic protein, at 183,020 to 184,000
GFF3363
GFF3364 - Rhodanese-related sulfurtransferase, at 184,099 to 185,724
GFF3364
GFF3365 - Cell division topological specificity factor MinE, at 185,702 to 185,980
GFF3365
GFF3366 - Septum site-determining protein MinD, at 185,984 to 186,799
GFF3366
Position (kb)
184
185
186 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 183.107 kb on + strand at 183.263 kb on + strand, within GFF3363 at 183.290 kb on + strand, within GFF3363 at 183.290 kb on + strand, within GFF3363 at 183.356 kb on - strand, within GFF3363 at 183.357 kb on - strand, within GFF3363 at 183.357 kb on - strand, within GFF3363 at 183.357 kb on - strand, within GFF3363 at 183.494 kb on + strand, within GFF3363 at 183.494 kb on + strand, within GFF3363 at 183.494 kb on + strand, within GFF3363 at 183.495 kb on - strand, within GFF3363 at 183.515 kb on + strand, within GFF3363 at 183.701 kb on + strand, within GFF3363 at 183.701 kb on + strand, within GFF3363 at 183.701 kb on + strand, within GFF3363 at 183.701 kb on + strand, within GFF3363 at 183.702 kb on - strand, within GFF3363 at 183.783 kb on - strand, within GFF3363 at 183.881 kb on + strand, within GFF3363 at 184.005 kb on + strand at 184.006 kb on - strand at 184.190 kb on - strand at 184.253 kb on + strand at 184.253 kb on + strand at 184.254 kb on - strand at 184.254 kb on - strand at 184.254 kb on - strand at 184.294 kb on + strand, within GFF3364 at 184.294 kb on + strand, within GFF3364 at 184.360 kb on + strand, within GFF3364 at 184.360 kb on + strand, within GFF3364 at 184.361 kb on - strand, within GFF3364 at 184.361 kb on - strand, within GFF3364 at 184.477 kb on + strand, within GFF3364 at 184.942 kb on + strand, within GFF3364 at 184.942 kb on + strand, within GFF3364 at 184.942 kb on + strand, within GFF3364 at 184.942 kb on + strand, within GFF3364 at 184.942 kb on + strand, within GFF3364 at 184.943 kb on - strand, within GFF3364 at 184.943 kb on - strand, within GFF3364 at 184.943 kb on - strand, within GFF3364 at 185.003 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.146 kb on + strand, within GFF3364 at 185.147 kb on - strand, within GFF3364 at 185.147 kb on - strand, within GFF3364 at 185.147 kb on - strand, within GFF3364 at 185.147 kb on - strand, within GFF3364 at 185.147 kb on - strand, within GFF3364 at 185.253 kb on + strand, within GFF3364 at 185.299 kb on + strand, within GFF3364 at 185.329 kb on + strand, within GFF3364 at 185.329 kb on + strand, within GFF3364 at 185.330 kb on - strand, within GFF3364 at 185.330 kb on - strand, within GFF3364 at 185.330 kb on - strand, within GFF3364 at 185.330 kb on - strand, within GFF3364 at 185.330 kb on - strand, within GFF3364 at 185.330 kb on - strand, within GFF3364 at 185.330 kb on - strand, within GFF3364 at 185.472 kb on - strand, within GFF3364 at 185.472 kb on - strand, within GFF3364 at 185.536 kb on + strand, within GFF3364 at 185.536 kb on + strand, within GFF3364 at 185.536 kb on + strand, within GFF3364 at 185.536 kb on + strand, within GFF3364 at 185.536 kb on + strand, within GFF3364 at 185.536 kb on + strand, within GFF3364 at 185.537 kb on - strand, within GFF3364 at 185.537 kb on - strand, within GFF3364 at 185.537 kb on - strand, within GFF3364 at 185.537 kb on - strand, within GFF3364 at 185.630 kb on - strand at 186.084 kb on + strand, within GFF3366 at 186.084 kb on + strand, within GFF3366 at 186.084 kb on + strand, within GFF3366 at 186.085 kb on - strand, within GFF3366 at 186.252 kb on + strand, within GFF3366 at 186.252 kb on + strand, within GFF3366 at 186.253 kb on - strand, within GFF3366 at 186.253 kb on - strand, within GFF3366 at 186.253 kb on - strand, within GFF3366 at 186.253 kb on - strand, within GFF3366 at 186.253 kb on - strand, within GFF3366 at 186.453 kb on + strand, within GFF3366 at 186.453 kb on + strand, within GFF3366 at 186.453 kb on + strand, within GFF3366 at 186.453 kb on + strand, within GFF3366 at 186.454 kb on - strand, within GFF3366 at 186.454 kb on - strand, within GFF3366 at 186.454 kb on - strand, within GFF3366 at 186.454 kb on - strand, within GFF3366 at 186.454 kb on - strand, within GFF3366 at 186.627 kb on + strand, within GFF3366 at 186.627 kb on + strand, within GFF3366 at 186.627 kb on + strand, within GFF3366 at 186.627 kb on + strand, within GFF3366 at 186.628 kb on - strand, within GFF3366 at 186.628 kb on - strand, within GFF3366 at 186.628 kb on - strand, within GFF3366
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 183,107 + +0.3 183,263 + GFF3363 0.25 -1.3 183,290 + GFF3363 0.28 -0.2 183,290 + GFF3363 0.28 -0.7 183,356 - GFF3363 0.34 +0.3 183,357 - GFF3363 0.34 -0.2 183,357 - GFF3363 0.34 +0.1 183,357 - GFF3363 0.34 +0.5 183,494 + GFF3363 0.48 -1.4 183,494 + GFF3363 0.48 -0.5 183,494 + GFF3363 0.48 -0.4 183,495 - GFF3363 0.48 -0.4 183,515 + GFF3363 0.50 -0.4 183,701 + GFF3363 0.69 +1.4 183,701 + GFF3363 0.69 -0.2 183,701 + GFF3363 0.69 -0.3 183,701 + GFF3363 0.69 +1.6 183,702 - GFF3363 0.70 -0.5 183,783 - GFF3363 0.78 -0.9 183,881 + GFF3363 0.88 -0.6 184,005 + +0.3 184,006 - +0.3 184,190 - -1.0 184,253 + +1.0 184,253 + +0.0 184,254 - -0.6 184,254 - +2.4 184,254 - -0.5 184,294 + GFF3364 0.12 -0.7 184,294 + GFF3364 0.12 +0.1 184,360 + GFF3364 0.16 +1.0 184,360 + GFF3364 0.16 -0.0 184,361 - GFF3364 0.16 +1.8 184,361 - GFF3364 0.16 -0.4 184,477 + GFF3364 0.23 -0.4 184,942 + GFF3364 0.52 +1.0 184,942 + GFF3364 0.52 -0.7 184,942 + GFF3364 0.52 +0.0 184,942 + GFF3364 0.52 +0.2 184,942 + GFF3364 0.52 -0.2 184,943 - GFF3364 0.52 +0.4 184,943 - GFF3364 0.52 +0.4 184,943 - GFF3364 0.52 +0.0 185,003 + GFF3364 0.56 -0.2 185,146 + GFF3364 0.64 -0.4 185,146 + GFF3364 0.64 +0.7 185,146 + GFF3364 0.64 +0.4 185,146 + GFF3364 0.64 -0.2 185,146 + GFF3364 0.64 -0.2 185,146 + GFF3364 0.64 -0.1 185,146 + GFF3364 0.64 +0.1 185,146 + GFF3364 0.64 -0.7 185,147 - GFF3364 0.64 +0.7 185,147 - GFF3364 0.64 -0.3 185,147 - GFF3364 0.64 +0.3 185,147 - GFF3364 0.64 +1.5 185,147 - GFF3364 0.64 +0.0 185,253 + GFF3364 0.71 +1.3 185,299 + GFF3364 0.74 -0.6 185,329 + GFF3364 0.76 +0.4 185,329 + GFF3364 0.76 +1.4 185,330 - GFF3364 0.76 -0.4 185,330 - GFF3364 0.76 +1.0 185,330 - GFF3364 0.76 -0.5 185,330 - GFF3364 0.76 +1.8 185,330 - GFF3364 0.76 +0.3 185,330 - GFF3364 0.76 -0.2 185,330 - GFF3364 0.76 +1.7 185,472 - GFF3364 0.84 +1.5 185,472 - GFF3364 0.84 -0.6 185,536 + GFF3364 0.88 -1.0 185,536 + GFF3364 0.88 -0.3 185,536 + GFF3364 0.88 -0.1 185,536 + GFF3364 0.88 -0.8 185,536 + GFF3364 0.88 -0.2 185,536 + GFF3364 0.88 -0.5 185,537 - GFF3364 0.88 +0.3 185,537 - GFF3364 0.88 +1.4 185,537 - GFF3364 0.88 +0.4 185,537 - GFF3364 0.88 -2.0 185,630 - +0.0 186,084 + GFF3366 0.12 -1.5 186,084 + GFF3366 0.12 -0.1 186,084 + GFF3366 0.12 +0.4 186,085 - GFF3366 0.12 -0.2 186,252 + GFF3366 0.33 -0.6 186,252 + GFF3366 0.33 +1.5 186,253 - GFF3366 0.33 -0.0 186,253 - GFF3366 0.33 -0.9 186,253 - GFF3366 0.33 +0.1 186,253 - GFF3366 0.33 +1.8 186,253 - GFF3366 0.33 -0.2 186,453 + GFF3366 0.57 +0.8 186,453 + GFF3366 0.57 +0.3 186,453 + GFF3366 0.57 -1.5 186,453 + GFF3366 0.57 -0.0 186,454 - GFF3366 0.58 +0.2 186,454 - GFF3366 0.58 -0.7 186,454 - GFF3366 0.58 -0.5 186,454 - GFF3366 0.58 +0.1 186,454 - GFF3366 0.58 -1.0 186,627 + GFF3366 0.79 -0.1 186,627 + GFF3366 0.79 +0.3 186,627 + GFF3366 0.79 +0.2 186,627 + GFF3366 0.79 +0.7 186,628 - GFF3366 0.79 +0.5 186,628 - GFF3366 0.79 -0.2 186,628 - GFF3366 0.79 -0.2
Or see this region's nucleotide sequence