Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3353 and GFF3354 are separated by 31 nucleotides GFF3354 and GFF3355 are separated by 19 nucleotides GFF3355 and GFF3356 are separated by 204 nucleotides GFF3356 and GFF3357 are separated by 78 nucleotides
GFF3353 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 175,773 to 176,618
GFF3353
GFF3354 - FIG00537998: hypothetical protein, at 176,650 to 177,546
GFF3354
GFF3355 - no description, at 177,566 to 177,958
GFF3355
GFF3356 - Probable transmembrane protein, at 178,163 to 178,588
GFF3356
GFF3357 - hypothetical protein, at 178,667 to 178,921
GFF3357
Position (kb) 177
178 Strain fitness (log2 ratio) -1
0
1
2 at 176.570 kb on - strand at 176.580 kb on + strand at 176.581 kb on - strand at 176.803 kb on + strand, within GFF3354 at 176.803 kb on + strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 177.383 kb on - strand, within GFF3354 at 177.544 kb on + strand at 177.545 kb on - strand at 177.924 kb on + strand at 177.924 kb on + strand at 177.924 kb on + strand at 177.924 kb on + strand at 177.925 kb on - strand at 177.925 kb on - strand at 177.925 kb on - strand at 177.925 kb on - strand at 177.925 kb on - strand at 177.925 kb on - strand at 178.058 kb on + strand at 178.337 kb on + strand, within GFF3356 at 178.337 kb on + strand, within GFF3356 at 178.454 kb on + strand, within GFF3356 at 178.454 kb on + strand, within GFF3356 at 178.454 kb on + strand, within GFF3356 at 178.454 kb on + strand, within GFF3356 at 178.455 kb on - strand, within GFF3356 at 178.455 kb on - strand, within GFF3356 at 178.455 kb on - strand, within GFF3356 at 178.455 kb on - strand, within GFF3356 at 178.455 kb on - strand, within GFF3356 at 178.455 kb on - strand, within GFF3356 at 178.506 kb on - strand, within GFF3356 at 178.607 kb on - strand at 178.607 kb on - strand at 178.767 kb on + strand, within GFF3357 at 178.767 kb on + strand, within GFF3357 at 178.767 kb on + strand, within GFF3357 at 178.767 kb on + strand, within GFF3357 at 178.767 kb on + strand, within GFF3357 at 178.767 kb on + strand, within GFF3357 at 178.767 kb on + strand, within GFF3357 at 178.768 kb on - strand, within GFF3357 at 178.952 kb on + strand at 178.952 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 176,570 - +0.0 176,580 + +1.3 176,581 - +0.3 176,803 + GFF3354 0.17 -0.1 176,803 + GFF3354 0.17 -0.5 176,804 - GFF3354 0.17 -0.5 176,804 - GFF3354 0.17 -1.0 176,804 - GFF3354 0.17 +0.3 176,804 - GFF3354 0.17 +0.2 177,383 - GFF3354 0.82 -0.9 177,544 + +0.8 177,545 - -1.3 177,924 + +0.3 177,924 + -0.7 177,924 + -0.2 177,924 + -0.5 177,925 - +1.8 177,925 - +0.4 177,925 - -0.0 177,925 - +1.8 177,925 - +0.8 177,925 - -0.4 178,058 + -0.1 178,337 + GFF3356 0.41 -0.5 178,337 + GFF3356 0.41 -0.3 178,454 + GFF3356 0.68 -0.3 178,454 + GFF3356 0.68 +0.6 178,454 + GFF3356 0.68 -0.6 178,454 + GFF3356 0.68 +0.1 178,455 - GFF3356 0.69 -0.5 178,455 - GFF3356 0.69 -0.4 178,455 - GFF3356 0.69 -1.0 178,455 - GFF3356 0.69 -0.5 178,455 - GFF3356 0.69 -0.6 178,455 - GFF3356 0.69 -0.4 178,506 - GFF3356 0.81 +0.1 178,607 - +0.3 178,607 - -0.5 178,767 + GFF3357 0.39 -1.1 178,767 + GFF3357 0.39 -0.4 178,767 + GFF3357 0.39 -1.0 178,767 + GFF3357 0.39 +0.8 178,767 + GFF3357 0.39 -0.4 178,767 + GFF3357 0.39 -0.2 178,767 + GFF3357 0.39 +0.3 178,768 - GFF3357 0.40 +0.6 178,952 + -0.6 178,952 + +1.6
Or see this region's nucleotide sequence