Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3334 and GFF3335 are separated by 16 nucleotides GFF3335 and GFF3336 are separated by 0 nucleotides GFF3336 and GFF3337 are separated by 38 nucleotides GFF3337 and GFF3338 are separated by 139 nucleotides
GFF3334 - hypothetical protein, at 158,935 to 159,822
GFF3334
GFF3335 - no description, at 159,839 to 159,997
GFF3335
GFF3336 - tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmG, at 159,998 to 161,947
GFF3336
GFF3337 - 16S rRNA (guanine(527)-N(7))-methyltransferase (EC 2.1.1.170), at 161,986 to 162,585
GFF3337
GFF3338 - Threonine efflux protein, at 162,725 to 163,339
GFF3338
Position (kb)
159
160
161
162 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 159.151 kb on + strand, within GFF3334 at 159.151 kb on + strand, within GFF3334 at 159.151 kb on + strand, within GFF3334 at 159.151 kb on + strand, within GFF3334 at 159.151 kb on + strand, within GFF3334 at 159.151 kb on + strand, within GFF3334 at 159.151 kb on + strand, within GFF3334 at 159.152 kb on - strand, within GFF3334 at 159.152 kb on - strand, within GFF3334 at 159.152 kb on - strand, within GFF3334 at 159.152 kb on - strand, within GFF3334 at 159.152 kb on - strand, within GFF3334 at 159.152 kb on - strand, within GFF3334 at 159.152 kb on - strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.241 kb on + strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.242 kb on - strand, within GFF3334 at 159.260 kb on - strand, within GFF3334 at 159.370 kb on + strand, within GFF3334 at 159.371 kb on - strand, within GFF3334 at 159.371 kb on - strand, within GFF3334 at 159.371 kb on - strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.508 kb on + strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.509 kb on - strand, within GFF3334 at 159.901 kb on + strand, within GFF3335 at 159.901 kb on + strand, within GFF3335 at 159.902 kb on - strand, within GFF3335 at 159.941 kb on + strand, within GFF3335 at 159.942 kb on - strand, within GFF3335 at 161.054 kb on + strand, within GFF3336 at 161.241 kb on - strand, within GFF3336 at 161.291 kb on + strand, within GFF3336 at 161.700 kb on + strand, within GFF3336 at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.955 kb on + strand at 161.956 kb on - strand at 161.956 kb on - strand at 161.956 kb on - strand at 161.956 kb on - strand at 161.961 kb on + strand at 161.961 kb on + strand at 161.961 kb on + strand at 161.962 kb on - strand at 161.962 kb on - strand at 161.962 kb on - strand at 161.962 kb on - strand at 161.962 kb on - strand at 162.031 kb on + strand at 162.031 kb on + strand at 162.031 kb on + strand at 162.032 kb on - strand at 162.032 kb on - strand at 162.064 kb on + strand, within GFF3337 at 162.064 kb on + strand, within GFF3337 at 162.064 kb on + strand, within GFF3337 at 162.064 kb on + strand, within GFF3337 at 162.064 kb on + strand, within GFF3337 at 162.065 kb on - strand, within GFF3337 at 162.065 kb on - strand, within GFF3337 at 162.065 kb on - strand, within GFF3337 at 162.065 kb on - strand, within GFF3337 at 162.065 kb on - strand, within GFF3337 at 162.065 kb on - strand, within GFF3337 at 162.065 kb on - strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.358 kb on + strand, within GFF3337 at 162.359 kb on + strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.359 kb on - strand, within GFF3337 at 162.459 kb on + strand, within GFF3337 at 162.459 kb on + strand, within GFF3337 at 162.459 kb on + strand, within GFF3337 at 162.459 kb on + strand, within GFF3337 at 162.460 kb on - strand, within GFF3337 at 162.460 kb on - strand, within GFF3337 at 162.460 kb on - strand, within GFF3337 at 162.611 kb on - strand at 162.668 kb on + strand at 162.669 kb on - strand at 162.669 kb on - strand at 162.669 kb on - strand at 162.743 kb on + strand at 162.743 kb on + strand at 162.744 kb on - strand at 162.744 kb on - strand at 162.930 kb on - strand, within GFF3338
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 159,151 + GFF3334 0.24 +0.1 159,151 + GFF3334 0.24 -0.1 159,151 + GFF3334 0.24 -0.3 159,151 + GFF3334 0.24 +0.3 159,151 + GFF3334 0.24 +0.4 159,151 + GFF3334 0.24 -0.3 159,151 + GFF3334 0.24 +0.3 159,152 - GFF3334 0.24 -0.2 159,152 - GFF3334 0.24 -0.7 159,152 - GFF3334 0.24 +1.6 159,152 - GFF3334 0.24 -0.2 159,152 - GFF3334 0.24 +0.3 159,152 - GFF3334 0.24 +0.5 159,152 - GFF3334 0.24 -0.4 159,241 + GFF3334 0.34 -0.6 159,241 + GFF3334 0.34 -0.5 159,241 + GFF3334 0.34 +0.1 159,241 + GFF3334 0.34 +0.1 159,241 + GFF3334 0.34 +0.3 159,241 + GFF3334 0.34 +0.1 159,241 + GFF3334 0.34 +1.6 159,241 + GFF3334 0.34 +0.0 159,241 + GFF3334 0.34 -1.6 159,242 - GFF3334 0.35 -0.3 159,242 - GFF3334 0.35 -0.4 159,242 - GFF3334 0.35 -1.5 159,242 - GFF3334 0.35 -1.0 159,242 - GFF3334 0.35 -0.2 159,242 - GFF3334 0.35 +0.9 159,242 - GFF3334 0.35 -0.3 159,242 - GFF3334 0.35 -0.9 159,242 - GFF3334 0.35 +0.3 159,242 - GFF3334 0.35 -0.5 159,242 - GFF3334 0.35 -1.0 159,242 - GFF3334 0.35 +0.3 159,242 - GFF3334 0.35 +0.0 159,242 - GFF3334 0.35 -0.8 159,242 - GFF3334 0.35 -0.2 159,242 - GFF3334 0.35 -0.6 159,242 - GFF3334 0.35 +0.5 159,260 - GFF3334 0.37 +0.7 159,370 + GFF3334 0.49 +0.5 159,371 - GFF3334 0.49 +0.1 159,371 - GFF3334 0.49 -0.8 159,371 - GFF3334 0.49 -0.1 159,508 + GFF3334 0.65 -0.2 159,508 + GFF3334 0.65 +0.2 159,508 + GFF3334 0.65 +0.3 159,508 + GFF3334 0.65 +0.2 159,508 + GFF3334 0.65 +0.8 159,508 + GFF3334 0.65 -0.2 159,508 + GFF3334 0.65 -1.2 159,508 + GFF3334 0.65 -0.5 159,508 + GFF3334 0.65 +0.0 159,508 + GFF3334 0.65 +1.4 159,508 + GFF3334 0.65 +0.8 159,508 + GFF3334 0.65 -1.0 159,508 + GFF3334 0.65 -0.0 159,508 + GFF3334 0.65 -0.1 159,509 - GFF3334 0.65 -0.6 159,509 - GFF3334 0.65 -0.1 159,509 - GFF3334 0.65 +0.8 159,509 - GFF3334 0.65 -0.6 159,509 - GFF3334 0.65 -0.2 159,509 - GFF3334 0.65 +3.1 159,509 - GFF3334 0.65 -0.0 159,509 - GFF3334 0.65 -0.5 159,509 - GFF3334 0.65 +0.3 159,509 - GFF3334 0.65 +0.5 159,509 - GFF3334 0.65 -0.6 159,509 - GFF3334 0.65 -0.2 159,509 - GFF3334 0.65 -0.2 159,901 + GFF3335 0.39 -0.7 159,901 + GFF3335 0.39 -0.1 159,902 - GFF3335 0.40 +1.3 159,941 + GFF3335 0.64 -0.0 159,942 - GFF3335 0.65 +0.6 161,054 + GFF3336 0.54 -0.2 161,241 - GFF3336 0.64 +0.3 161,291 + GFF3336 0.66 -0.5 161,700 + GFF3336 0.87 +0.3 161,955 + -0.2 161,955 + +0.2 161,955 + -0.5 161,955 + +0.8 161,955 + -0.5 161,955 + +1.6 161,955 + -0.7 161,955 + -0.8 161,955 + +0.7 161,955 + +0.0 161,955 + -0.3 161,956 - +0.4 161,956 - +2.4 161,956 - +0.6 161,956 - +0.4 161,961 + +1.4 161,961 + +0.2 161,961 + -0.0 161,962 - -0.6 161,962 - +0.2 161,962 - +0.8 161,962 - -0.5 161,962 - +0.4 162,031 + +0.5 162,031 + +0.8 162,031 + -1.2 162,032 - +0.8 162,032 - -0.7 162,064 + GFF3337 0.13 -0.5 162,064 + GFF3337 0.13 +0.3 162,064 + GFF3337 0.13 +0.7 162,064 + GFF3337 0.13 -0.2 162,064 + GFF3337 0.13 -1.3 162,065 - GFF3337 0.13 -0.7 162,065 - GFF3337 0.13 -0.5 162,065 - GFF3337 0.13 -0.0 162,065 - GFF3337 0.13 +0.1 162,065 - GFF3337 0.13 +0.4 162,065 - GFF3337 0.13 -0.9 162,065 - GFF3337 0.13 +0.8 162,358 + GFF3337 0.62 -0.5 162,358 + GFF3337 0.62 +0.4 162,358 + GFF3337 0.62 -0.4 162,358 + GFF3337 0.62 -0.4 162,358 + GFF3337 0.62 -2.9 162,358 + GFF3337 0.62 +0.3 162,358 + GFF3337 0.62 -0.4 162,358 + GFF3337 0.62 -0.3 162,358 + GFF3337 0.62 +1.1 162,358 + GFF3337 0.62 +0.8 162,358 + GFF3337 0.62 -0.5 162,358 + GFF3337 0.62 -0.4 162,358 + GFF3337 0.62 +0.8 162,358 + GFF3337 0.62 -1.5 162,358 + GFF3337 0.62 +0.1 162,358 + GFF3337 0.62 +1.7 162,358 + GFF3337 0.62 -0.8 162,358 + GFF3337 0.62 +1.4 162,358 + GFF3337 0.62 +0.3 162,358 + GFF3337 0.62 -0.4 162,358 + GFF3337 0.62 +0.3 162,358 + GFF3337 0.62 +0.3 162,358 + GFF3337 0.62 -0.2 162,358 + GFF3337 0.62 -0.2 162,358 + GFF3337 0.62 -0.6 162,358 + GFF3337 0.62 -0.3 162,358 + GFF3337 0.62 +2.4 162,358 + GFF3337 0.62 +0.6 162,358 + GFF3337 0.62 +0.3 162,358 + GFF3337 0.62 -0.0 162,358 + GFF3337 0.62 -0.4 162,359 + GFF3337 0.62 +0.8 162,359 - GFF3337 0.62 -1.2 162,359 - GFF3337 0.62 +0.0 162,359 - GFF3337 0.62 -0.6 162,359 - GFF3337 0.62 +0.6 162,359 - GFF3337 0.62 -0.6 162,359 - GFF3337 0.62 -0.9 162,359 - GFF3337 0.62 -0.1 162,359 - GFF3337 0.62 -0.2 162,359 - GFF3337 0.62 -1.5 162,359 - GFF3337 0.62 +1.4 162,359 - GFF3337 0.62 -0.2 162,359 - GFF3337 0.62 -0.9 162,359 - GFF3337 0.62 -0.3 162,359 - GFF3337 0.62 -1.6 162,359 - GFF3337 0.62 -0.2 162,359 - GFF3337 0.62 -0.2 162,359 - GFF3337 0.62 -0.6 162,359 - GFF3337 0.62 +0.1 162,359 - GFF3337 0.62 -1.0 162,359 - GFF3337 0.62 -1.7 162,359 - GFF3337 0.62 -0.2 162,359 - GFF3337 0.62 +0.4 162,359 - GFF3337 0.62 +0.0 162,359 - GFF3337 0.62 -1.2 162,359 - GFF3337 0.62 -0.6 162,359 - GFF3337 0.62 +0.2 162,359 - GFF3337 0.62 +0.5 162,459 + GFF3337 0.79 +0.4 162,459 + GFF3337 0.79 -0.5 162,459 + GFF3337 0.79 +0.3 162,459 + GFF3337 0.79 +0.8 162,460 - GFF3337 0.79 -0.5 162,460 - GFF3337 0.79 +0.2 162,460 - GFF3337 0.79 -0.7 162,611 - +0.3 162,668 + -0.6 162,669 - +1.5 162,669 - -0.0 162,669 - +0.4 162,743 + +0.6 162,743 + +1.8 162,744 - -0.5 162,744 - -0.6 162,930 - GFF3338 0.33 +0.6
Or see this region's nucleotide sequence