Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF331 and GFF332 are separated by 2 nucleotides GFF332 and GFF333 are separated by 9 nucleotides GFF333 and GFF334 overlap by 28 nucleotides GFF334 and GFF335 are separated by 84 nucleotides
GFF331 - Nucleoside ABC transporter, permease protein 2, at 365,735 to 366,658
GFF331
GFF332 - hypothetical protein, at 366,661 to 367,122
GFF332
GFF333 - hypothetical protein, at 367,132 to 367,584
GFF333
GFF334 - Transcriptional regulator, AraC family, at 367,557 to 368,390
GFF334
GFF335 - Cytosine deaminase (EC 3.5.4.1), at 368,475 to 369,755
GFF335
Position (kb) 367
368 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 366.218 kb on + strand, within GFF331 at 366.219 kb on - strand, within GFF331 at 366.542 kb on + strand, within GFF331 at 366.551 kb on + strand, within GFF331 at 366.551 kb on + strand, within GFF331 at 366.551 kb on + strand, within GFF331 at 366.551 kb on + strand, within GFF331 at 366.551 kb on + strand, within GFF331 at 366.551 kb on + strand, within GFF331 at 366.552 kb on - strand, within GFF331 at 366.552 kb on - strand, within GFF331 at 366.552 kb on - strand, within GFF331 at 366.552 kb on - strand, within GFF331 at 366.552 kb on - strand, within GFF331 at 366.566 kb on + strand at 366.567 kb on - strand at 366.567 kb on - strand at 366.671 kb on + strand at 366.672 kb on - strand at 366.758 kb on - strand, within GFF332 at 366.758 kb on - strand, within GFF332 at 366.835 kb on + strand, within GFF332 at 366.835 kb on + strand, within GFF332 at 366.835 kb on + strand, within GFF332 at 366.835 kb on + strand, within GFF332 at 366.835 kb on + strand, within GFF332 at 366.836 kb on - strand, within GFF332 at 366.877 kb on + strand, within GFF332 at 366.877 kb on + strand, within GFF332 at 366.877 kb on + strand, within GFF332 at 366.877 kb on + strand, within GFF332 at 366.878 kb on - strand, within GFF332 at 366.878 kb on - strand, within GFF332 at 367.093 kb on + strand at 367.121 kb on - strand at 367.421 kb on - strand, within GFF333 at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.548 kb on + strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.549 kb on - strand at 367.552 kb on + strand at 367.552 kb on + strand at 367.552 kb on + strand at 367.552 kb on + strand at 367.553 kb on - strand at 367.553 kb on - strand at 367.553 kb on - strand at 367.553 kb on - strand at 367.553 kb on - strand at 367.553 kb on - strand at 367.553 kb on - strand at 367.553 kb on - strand at 367.582 kb on + strand at 367.582 kb on + strand at 367.645 kb on + strand, within GFF334 at 367.646 kb on - strand, within GFF334 at 367.646 kb on - strand, within GFF334 at 367.843 kb on + strand, within GFF334 at 367.843 kb on + strand, within GFF334 at 367.844 kb on - strand, within GFF334 at 367.844 kb on - strand, within GFF334 at 367.844 kb on - strand, within GFF334 at 367.844 kb on - strand, within GFF334 at 368.251 kb on + strand, within GFF334 at 368.252 kb on - strand, within GFF334
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 366,218 + GFF331 0.52 +0.1 366,219 - GFF331 0.52 +0.1 366,542 + GFF331 0.87 +2.8 366,551 + GFF331 0.88 +0.6 366,551 + GFF331 0.88 +0.1 366,551 + GFF331 0.88 -1.1 366,551 + GFF331 0.88 -1.6 366,551 + GFF331 0.88 +0.4 366,551 + GFF331 0.88 +0.6 366,552 - GFF331 0.88 -0.7 366,552 - GFF331 0.88 +1.4 366,552 - GFF331 0.88 +0.4 366,552 - GFF331 0.88 +1.2 366,552 - GFF331 0.88 +0.9 366,566 + +0.1 366,567 - +0.3 366,567 - +0.9 366,671 + -0.1 366,672 - -0.3 366,758 - GFF332 0.21 +0.5 366,758 - GFF332 0.21 -0.2 366,835 + GFF332 0.38 +0.0 366,835 + GFF332 0.38 -1.7 366,835 + GFF332 0.38 -0.4 366,835 + GFF332 0.38 -0.0 366,835 + GFF332 0.38 +0.4 366,836 - GFF332 0.38 +0.3 366,877 + GFF332 0.47 +0.6 366,877 + GFF332 0.47 -0.0 366,877 + GFF332 0.47 -0.1 366,877 + GFF332 0.47 +0.4 366,878 - GFF332 0.47 +1.2 366,878 - GFF332 0.47 -0.4 367,093 + +1.8 367,121 - +0.4 367,421 - GFF333 0.64 -0.0 367,548 + -0.6 367,548 + -0.6 367,548 + -1.0 367,548 + +0.4 367,548 + +0.5 367,548 + +0.2 367,548 + -0.8 367,548 + -0.4 367,548 + -0.7 367,548 + +0.2 367,548 + -0.5 367,549 - -0.5 367,549 - -0.2 367,549 - +1.9 367,549 - -0.8 367,549 - -0.5 367,549 - -0.8 367,549 - -2.8 367,549 - -0.3 367,549 - -0.4 367,549 - -0.3 367,549 - +1.2 367,552 + -0.4 367,552 + -0.3 367,552 + -0.8 367,552 + -0.5 367,553 - -0.2 367,553 - -0.3 367,553 - +0.9 367,553 - +0.0 367,553 - -0.4 367,553 - -0.4 367,553 - -0.4 367,553 - -1.4 367,582 + -0.3 367,582 + -0.1 367,645 + GFF334 0.11 -0.5 367,646 - GFF334 0.11 -0.4 367,646 - GFF334 0.11 -0.8 367,843 + GFF334 0.34 -0.5 367,843 + GFF334 0.34 -0.4 367,844 - GFF334 0.34 +1.0 367,844 - GFF334 0.34 +0.4 367,844 - GFF334 0.34 -1.2 367,844 - GFF334 0.34 +0.4 368,251 + GFF334 0.83 +0.1 368,252 - GFF334 0.83 +0.3
Or see this region's nucleotide sequence