Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3322 and GFF3323 are separated by 181 nucleotides GFF3323 and GFF3324 are separated by 7 nucleotides
GFF3322 - hypothetical protein, at 148,892 to 149,752
GFF3322
GFF3323 - Tricarboxylate transport transcriptional regulator TctD, at 149,934 to 150,578
GFF3323
GFF3324 - hypothetical protein, at 150,586 to 151,980
GFF3324
Position (kb) 149
150
151 Strain fitness (log2 ratio) -2
-1
0
1
2 at 149.001 kb on + strand, within GFF3322 at 149.001 kb on + strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.002 kb on - strand, within GFF3322 at 149.277 kb on + strand, within GFF3322 at 149.295 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.322 kb on + strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.323 kb on - strand, within GFF3322 at 149.583 kb on + strand, within GFF3322 at 149.584 kb on - strand, within GFF3322 at 149.613 kb on + strand, within GFF3322 at 149.789 kb on + strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.800 kb on + strand at 149.800 kb on + strand at 149.800 kb on + strand at 149.801 kb on - strand at 149.801 kb on - strand at 149.801 kb on - strand at 149.868 kb on + strand at 150.555 kb on + strand at 150.555 kb on + strand at 150.725 kb on - strand at 150.770 kb on - strand, within GFF3324 at 150.871 kb on + strand, within GFF3324 at 150.872 kb on - strand, within GFF3324 at 150.976 kb on + strand, within GFF3324 at 150.976 kb on + strand, within GFF3324 at 150.976 kb on + strand, within GFF3324 at 150.977 kb on - strand, within GFF3324 at 151.106 kb on - strand, within GFF3324 at 151.430 kb on + strand, within GFF3324 at 151.430 kb on + strand, within GFF3324 at 151.430 kb on + strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 149,001 + GFF3322 0.13 +0.0 149,001 + GFF3322 0.13 -1.1 149,002 - GFF3322 0.13 +2.3 149,002 - GFF3322 0.13 +1.8 149,002 - GFF3322 0.13 -0.5 149,002 - GFF3322 0.13 -0.2 149,002 - GFF3322 0.13 -0.6 149,277 + GFF3322 0.45 +0.3 149,295 + GFF3322 0.47 -0.5 149,322 + GFF3322 0.50 -0.3 149,322 + GFF3322 0.50 -0.2 149,322 + GFF3322 0.50 +0.5 149,322 + GFF3322 0.50 -2.0 149,322 + GFF3322 0.50 +0.1 149,322 + GFF3322 0.50 -0.2 149,323 - GFF3322 0.50 +0.8 149,323 - GFF3322 0.50 -0.5 149,323 - GFF3322 0.50 -0.5 149,323 - GFF3322 0.50 -0.2 149,323 - GFF3322 0.50 +0.2 149,583 + GFF3322 0.80 +0.6 149,584 - GFF3322 0.80 -0.7 149,613 + GFF3322 0.84 -1.0 149,789 + -0.2 149,790 - -0.5 149,790 - +0.3 149,790 - +0.3 149,790 - -0.5 149,790 - +0.4 149,790 - +1.8 149,790 - +0.4 149,800 + +0.5 149,800 + -0.2 149,800 + +1.8 149,801 - +0.8 149,801 - -0.3 149,801 - -1.5 149,868 + -0.5 150,555 + -0.6 150,555 + +1.3 150,725 - -0.8 150,770 - GFF3324 0.13 +1.8 150,871 + GFF3324 0.20 +0.8 150,872 - GFF3324 0.21 -0.2 150,976 + GFF3324 0.28 +1.8 150,976 + GFF3324 0.28 -1.4 150,976 + GFF3324 0.28 +2.4 150,977 - GFF3324 0.28 -0.9 151,106 - GFF3324 0.37 +0.0 151,430 + GFF3324 0.61 -0.2 151,430 + GFF3324 0.61 -0.1 151,430 + GFF3324 0.61 +0.1 151,431 - GFF3324 0.61 +0.0 151,431 - GFF3324 0.61 -0.1 151,431 - GFF3324 0.61 +0.1 151,431 - GFF3324 0.61 -0.0 151,431 - GFF3324 0.61 -0.4
Or see this region's nucleotide sequence