Strain Fitness in Variovorax sp. SCN45 around GFF3281

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3280 and GFF3281 are separated by 88 nucleotidesGFF3281 and GFF3282 are separated by 136 nucleotides GFF3280 - Chromosomal replication initiator protein DnaA, at 99,320 to 100,699 GFF3280 GFF3281 - DNA polymerase III beta subunit (EC 2.7.7.7), at 100,788 to 101,894 GFF3281 GFF3282 - DNA gyrase subunit B (EC 5.99.1.3), at 102,031 to 104,640 GFF3282 Position (kb) 100 101 102Strain fitness (log2 ratio) -2 -1 0 1at 100.749 kb on + strandat 100.749 kb on + strandat 100.749 kb on + strandat 100.749 kb on + strandat 100.749 kb on + strandat 100.749 kb on + strandat 100.749 kb on + strandat 100.749 kb on + strandat 100.749 kb on + strandat 100.750 kb on - strandat 100.750 kb on - strandat 100.750 kb on - strandat 100.750 kb on - strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 100.769 kb on + strandat 101.892 kb on + strandat 101.892 kb on + strandat 101.892 kb on + strandat 101.893 kb on - strandat 101.893 kb on - strandat 101.893 kb on - strandat 101.893 kb on - strandat 101.893 kb on - strandat 101.893 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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100,749 + +0.4
100,749 + -0.2
100,749 + +0.3
100,749 + -1.9
100,749 + -2.2
100,749 + -0.4
100,749 + -0.0
100,749 + -0.4
100,749 + -0.2
100,750 - -1.6
100,750 - -0.2
100,750 - -0.7
100,750 - +1.1
100,769 + -0.4
100,769 + +0.1
100,769 + -0.6
100,769 + -0.5
100,769 + +0.4
100,769 + -0.3
100,769 + -0.7
100,769 + -0.5
100,769 + +0.1
100,769 + -0.9
101,892 + -0.8
101,892 + -1.2
101,892 + +0.2
101,893 - +0.3
101,893 - +0.1
101,893 - +0.0
101,893 - -0.4
101,893 - -0.3
101,893 - -0.2

Or see this region's nucleotide sequence